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Clustering results showed that the clusters were not corresponding to heterotic groups and pedigrees,which suggested that the A-PAGE of seed storage proteins might not be a good method for large-scale analysis of genetic diversity and heterotic grouping in maize germplasm.

利用盐溶蛋白多态性完成的聚类分析结果和自交系系谱及杂种优势群之间没有明显的对应关系，表明储藏蛋白A-PAGE方法对于大规模玉米种质资源的遗传多样性分析和类群划分可能存在局限性。

Results of the histochemical and SDS-PAGE analysis showed that the target genes were stably inherited.

组织化学和 SDS-PAGE 检测结果表明，外源基因能够稳定遗传，功能得到正确表达。

The N gene ORF was then subcloned into pET-30a vector and the recombinant plasmid was transformed into E.coli BL21 (DE3) and induced with IPTG. The protein expression was determined by SDS-PAGE. The expressed protein had a molecular weight of 54.4 Ku that existed as inclusion body. Thin-layer scanning showed that the expression product accounted for 30.5% of the total bacterial proteins. The recombinant protein possessed native biological activity and could react with anti-PEDV hyperimmune serum in Western blot.

以阳性质粒为模板，用分别含有BamH Ⅰ和Xho Ⅰ酶切位点的上、下游引物扩增得到ORF，其PCR产物经BamH Ⅰ和Xho Ⅰ双酶切后定向克隆到pET-30a载体，构建的重组质粒命名为pET-30a-PN；将pET-30a-PN转化到大肠杆菌BL21 (DF3)中，在IPTG诱导下进行表达；SDS-PAGE结果表明表达出与预期大小相符的约54.4 Ku的重组蛋白，重组蛋白以包涵体形式存在；薄层扫描结果表明表达产物占菌体总蛋白的30.5%；Western blot分析表明表达的重组蛋白能与抗PEDV高免血清反应，说明该重组蛋白具有免疫学活性。

Methods The target cDNA was amplified by PCR and PCR products were indigested by restriction enzyme Nco Ⅰ and Hind Ⅲ. Then the indigested products were recombined into pET-BBH and pET-BBH was expressed in E. coli BL21 (DE3) by IPTG inducing. Next, the expressed proteins were identified by SDS-PAGE, western blot and enzyme activity test. Finally, fluorescence quantitative PCR was used to test its expression quantity in different worm stages.

PCR扩增GAMMA-BBH cDNA基因，产物经NcoⅠ和HindⅢ限制性内切酶酶切后连接至原核表达载体重组为pET-BBH，在BL21(DE3)中用IPTG诱导表达，SDS-PAGE、Western blot和酶活性测定鉴定表达产物，用荧光定量PCR方法检测广州管圆线虫不同虫龄GAMMA-BBH的表达量。

The result of SDS-PAGE indicated that this insecticidal protein contained two subunits.

SDS-PAGE图谱显示此杀虫毒素是由两个亚基组成的复合蛋白。

The dextran conjugated laccase had a molecular weight over 200×103, as determined by HPLC and SDS-PAGE.

HPLC和SDS-PAGE分析表明葡聚糖修饰漆酶的分子量在200×103以上。

E. coli cells harboring the recombinant plasmid was cultured with Laria broth medium and induced with isopropyl β-D-thiogalactopyranoside and detected from supernatant and precipitate of culture lysates by using SDS-PAGE.

将重组菌培养并经IPTG诱导后用超声波裂解菌体，取上清和沉淀同时作SDS-PAGE电泳后显示，上清中没有热休克蛋白A的表达，在沉淀中有热休克蛋白A的表达。

The major direction of the research within the first year will be outlined as following:( 1 )The background study, collection, analysis, and identification of the possible metabolites such as protease, hemolysin, hemagglutinin, cytotoxin, leukocidin, and lipopolysaccharide;( 2 )The collection of the pathogenic factor such as outer membrane protein of vibrio spp.;( 3 )The analysis of pathogenic factor through the application of SDS-PAGE technique; and ( 4 )The activity assay of outer membrane protein on substrate to characterize the possible function or study the reaction mechanism.

致病因子之收集：将利用生物化学之方法，以弧菌属细菌外膜蛋白质为目标，收集与溶血素相关之致病因子。3。致病因子之分析：将利用SDS-PAGE电泳，对所收集得到之致病因子进行分析，并估算其可能之分子量。4。致病因子之活性测试：将利用所纯化之外膜蛋白质进行对受质活性之测试，以确定其功能或可能之作用机制。

Results1. Preparation of rude extracts of Lumbricus Bimastus nucleases and its purification Two groups of nucleases with different characters lied in the DNA-casting SDS-PAGE.

结果1。双胸蚓组织中核酸酶粗提物的制备及核酸酶的纯化应用本室建立的SDS-PAGE-DNA功能胶测活方法对核酸酶提取过程进行监测。

Methods1. Preparation of rude extracts of Lumbricus Bimastus nucleases and its purification Using DNA-casting SDS-PAGE and agarose gel to monitor the activity of the nuclease in the process of extraction.

双胸蚓组织中核酸酶粗提物的制备及核酸酶的纯化以SDS-PAGE-DNA功能胶和琼脂糖凝胶电泳测活相结合作为双胸蚓组织核酸酶提取过程中核酸酶活性的监测体系。

I will endeavour to find you some assistance.

我尽力帮你找人帮忙。

At first I only know bruck is the idol of American younglings, afterwards I returned back to Taiwan ,even in Beijing last year ,I saw her poster everywhere, I was so surprised at her charm.

起初我只晓得布鲁克雷德丝是美国少男少女崇拜的偶像，后来回台湾，甚至去年在北京，居然也四处看见她的海报，才惊讶她的魅力之大。