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An IPGphor unit with immobile pH gradient strips was used in and the Ettan DALT Ⅱ system under constant power and temperature in SDS-PAGE. The 2-D gel was stained by both methods of coomassie blue and silver.

第一向采用固定pH值梯度胶条在IPGphor等电聚焦仪上进行等电聚焦，第二向在恒功率且恒温条件下于Ettan-DALTⅡ高通量电泳仪上进行SDS-PAGE电泳，以银染和考马斯亮蓝两种方法染色。

An IPGphorTM unit with immobile pH gradient strips was used in and the EttanTM DALT Ⅱ system under constant power and temperature in SDS-PAGE. The 2-D gel was stained by both methods of coomassie blue and silver.

第一向采用固定pH值梯度胶条在IPGphorTM等电聚焦仪上进行等电聚焦，第二向在恒功率且恒温条件下于Ettan-DALTTMⅡ高通量电泳仪上进行SDS-PAGE电泳，以银染和考马斯亮蓝两种方法染色。

After collecting the bacterial cells and breaking the cell by means of ultrasonic, the inclusion bodieswere extracted. The extracted products were denatured with 8 mol/L urea and purified through Ni-NTAcolumn. The purified products were renatured by dialyzation, and then were analyzed by SDS-PAGE.

在优化条件下诱导重组菌表达，收集菌体经超声波破碎和裂解后提取不溶性包涵体，以8mol／L尿素处理包涵体提取物并进行Ni-NTA亲和层析纯化，纯化表达产物经透析复性后以SDS-PAGE分析其复性效果。

SDS-PAGE showed that fusion proteins had a disulfide-bonded dimeric structure and existed as homodimer.

SDS-PAGE结果显示该蛋白具有通过二硫键结合的二聚体结构并以同源二聚体的形式存在。

5 Wheat-Thinopyrum intermedium alien disomic addition lines (Line 1,Line 4,Line 10,Line 14 and Line 15) were identified by using the methods of morphology,cytology,A-PAGE and RAPD analysis.

利用形态学、细胞学、A-PAGE和RAPD方法，对5个小麦-中间偃麦草双体异附加系Line1、Line4、Line10、Line14和Line15进行了鉴定。

SDS-PAGE showed ,these two proteins' molecular weight is respectively 26KDa and 18KDa,both of which do not have intermolecular disulphide group.

经SDS-PAGE鉴定，这两种蛋白质的分子量分别为26.0 KDa和18.0 KDa，在经还原剂二硫苏糖醇处理后的电泳结果表明这两种蛋白均为不含有分子间二硫键的蛋白单体。

The SDS-PAGE electrophoresis indicated that all engineered strains containing different expression plasmid could apparently express a 45 kD band specific for daunorubicin C-14 hydroxylase. The expression level of doxA gene was much higher when the doxA gene was under the control of promoter PermE. However, it was found that fd terminator had few effect on the expression of doxA gene.

SDS-PAGE蛋白电泳实验证明，上述五个菌株都能够表达大小约45 kD的柔红霉素C-14羟化酶蛋白，并发现以在PermE启动子控制下的doxA基因表达量相对较高，而终止子对＠中英文摘要 dd基因表达似乎没有影响。

After separation and purification of the expression products by FPLC,the electrophoric patterns of the various eluted peaks are not different with the culture products on SDS-PAGE.

产物经SDS-PAGE、免疫印迹试验与FPLC分离等分析，均表明该工程菌的共表达产物为AchR α-亚基结构域(相对分子质量为13×103)及其与GroES的络合物(23×103)。

There are experimental results as follows:(1) Eriophorum comosum Nees shows strong cold resistance in plasmalemma permeability research. The lethal temperature of Eriophorum comosum Nees is the lowest LT_(50 in comparison with another two plants and is lower than -11.5℃ in August 2005 and December 2005; Soluble protein content of each plant is rising before or after cold hardiness. By SDS-PAGE, Protein band is analysed. Band quantity of Eriophorum comosum Nees is the most and its band color is darkest.(2) Soluble sugar content of three plants after cold hardiness are higher than before cold hardiness, moreover soluble sugar content after cold hardiness shows a decline and there is a narrow range in Eriophorum comosum Nees; Free Proline accumulation is changed by cold stress, and cold resistance is relative to free Proline accumulation; chlorophyll content of both three plants, including chlorophyll a, chlorophyll b and chlorophyll a/b, show a decline tendency along with the descent of temperature. Cynodon dactylon is highly sensitive to cold stress.(3) The soil POD and urease activities' absolute value of Erioophorum comosum Nees and Pogonatherum panideumHack were more than that of Cynodon dactylon before or after low temperature stress, especially POD activities.

实验结果表明：(1)在膜透性的研究中，丛毛羊胡子草抗寒性最强，采用电导率法测得半致死温度LT_(50最低，丛毛羊胡子草抗寒锻炼前后半致死温度LT_(50均小于-11.5℃；在可溶性蛋白含量研究中，抗寒锻炼前后三种植物的可溶性蛋白含量均增加，同时采用SDS-PAGE法，通过对蛋白条带分析，以丛毛羊胡子草条带数最多且着色深；(2)在可溶性糖的研究中，三种植物经抗寒锻炼后叶片可溶性糖含量都比抗寒锻炼前高，且经过抗寒锻炼后三种植物可溶性糖均呈下降趋势，丛毛羊胡子草可溶性糖含量下降较小；从游离脯氨酸含量可知，低温的胁迫影响了植物叶片游离脯氨酸的含量变化，植物的抗寒性与游离脯氨酸有关；低温胁迫下三种植物叶绿素含量随着温度的下降，叶绿素含量、叶绿素a、叶绿素b和叶绿素a／b均呈下降趋势，狗牙根对低温胁迫最为敏感；(3)低温锻炼前后，丛毛羊胡子草和金发草的土壤过氧化物酶与脲酶活性的绝对值均大于狗牙根的，特别是过氧化物酶，说明丛毛羊胡子草和金发草的酶活性大于狗牙根的。

Methods Spermatocytes, early stage spermatids and the later stage spermatids were separated from the adult rat testis by the velocity gravitative sedimentation technique. Using the whole mount electron microscopy, SDS-PAGE and Southwestern blot, the morphology, components of NM-L-IF and the relationship between an A-T repetitive DNA fragment and the proteins of NM-L-IF in those cells were analyzed.

利用整装电镜技术、SDS-PAGE和Southwestern blot等技术观察与分析经单位重力沉降法分离的大鼠精母细胞、早期精子细胞和晚期精子细胞的NM-L-IF的形态结构、蛋白组成及其与A-T重复序列的相互关系。

I will endeavour to find you some assistance.

我尽力帮你找人帮忙。

At first I only know bruck is the idol of American younglings, afterwards I returned back to Taiwan ,even in Beijing last year ,I saw her poster everywhere, I was so surprised at her charm.

起初我只晓得布鲁克雷德丝是美国少男少女崇拜的偶像，后来回台湾，甚至去年在北京，居然也四处看见她的海报，才惊讶她的魅力之大。