查询词典 page

Results: SDS-PAGE analysis revealed that Amaranthus spinosus pollen proteins comprised at lease eleven discrete protein bands with the molecular weight ranging from 15 kD to 80 kD, nine main bands with the molecular weight being 18 kD, 24 kD, 29 kD, 35 kD, 39 kD, 40 kD, 42 kD, 62 kD, 78 kD respectively.

结果：SDS-PAGE显示可辨蛋白条带有11条，分子量在15~80 kD之间，其中主带有9条，分子量依次为18 kD、24 kD、29 kD、35 kD、39 kD、40 kD、42 kD、62 kD、78 kD。

Get high purity DCs by Cultured plastic-adherent monocytes isolated from healthy human peripheral blood with GM-CSF and IL-4 for 7 days. To observe the morphology of DCs by inverted phase contrast microscope ,electron microscope and laser confocal microscope. Analyse phenotype of DCs with flow cytometry. Investigate the endocytosis ability of DCs as a group by Horseradish peroxidase endocytosis assay. To appraise allogeneic mixed lymphocytes reaction of DCs by MTT reduction assay. Analyse the levels of IL-12 and TNF in liquids of cultured medium by ELISA and MTT reduction assay respectively. Soluble antigens of HCCs was obtained by 3 freeze-and-thaw cycles. Biological characteristics of HC soluble antigens pulsed DCs were monitored by flow cytometry. According to MTT reduction assay estimated the cell proliferation of self lymphocytes activated by HC antigens pulsed DCs. Get high purity BCG HSP 70 protein by SDS-PAGE electrophoresis and determined its biological activity with ELISA. Analyse phenotype of antigen pulsed DCs primed by BCG HSP70 with flow cytometry. By MTT reduction assay estimated the cell proliferation of self lymphocytes and the MLR of DC based vaccine. Analyse expression of HLA-DR molecule on surface of HCC lines. The IFN-γ mRNA in lymphocytes after actived by DC vaccine and the Fas-L expression on DC and DC vaccine primed lymphocytes were detected by in situ hybridization and flow cytometry respectively. Specific cytotoxity lysis of T lymphocytes and nonspecific inhibition of liquids in culture medium against HCC lines were also tested. Detect expression of hAFP on four HCC lines with Cell-ELISA. Induce apoptosis of HCCs with actinomycin-D. Interaction of DCs and apoptotic cells was observed under transmission electron microscope. Growth inhibition test of DC against HCC lines was also performed. Establish the nude mouse model bearing human HC xenografts and indentify the characteristic of tumour by histochemistry and immunohistochemistry techniques. Prevent and treat transplanted human HC on nude mouse with Freezing and anabiotic HC specific lymphocytes.

用GM-CSF和IL-4从健康人外周血诱导DC；分别用倒置相差显微镜、电子显微镜及激光共聚焦显微镜观察DC形态；流式细胞术检测DC表型；HRP吞噬实验测定DC的群体内吞能力；MTT法检测同种异体混合淋巴细胞反应；ELISA法和MTT法分别测定DC培养上清液中IL-12和TNF水平；冻融法制备肝癌细胞可溶性抗原；流式细胞术检测负载肝癌可溶性抗原后DC的生物学特性；MTT法检测DC负载肝癌抗原后对自身淋巴细胞增殖的影响；SDS-PAGE制备电泳纯化BCG HSP70并鉴定纯度，ELISA测定活性；流式细胞术检测负载抗原DC经BCGHSP 70活化后的表型；MTT法检测肝癌DC疫苗对自身淋巴细胞增殖的影响和混合淋巴细胞反应；流式细胞术检测肝癌细胞表面HLA-DR表达；MTT法检测肝癌DC疫苗对自身淋巴细胞的活化；原位杂交法检测肝癌DC疫苗活化后的淋巴细胞IFN-γmRNA表达；流式细胞术检测DC和肝癌DC疫苗活化后淋巴细胞表面Fas-L；MTT法分别检测肝癌DC疫苗活化的淋巴细胞和其培养上清对肝癌细胞的特异性杀伤和非特异性抑制作用；Cell-ELISA检测人肝癌细胞hAFP表达；MTT法检测负载AFP表位肽和凋亡肝癌细胞DC对自身淋巴细胞增殖的影响；ELISA法和MTT法分别测定活化后淋巴细胞培养上清中TNF和IL-12水平；肝癌细胞凋亡的诱导和检测；DC吞噬凋亡肝癌细胞后的电子显微镜观察；DC对肝癌细胞的生长抑制试验；人肝癌裸鼠皮下移植瘤动物模型的建立及其组织学和免疫组织化学鉴定；DC及肝癌特异性淋巴细胞预防和治疗人肝癌裸鼠皮下移植瘤；冻存和复苏后的肝癌特异性淋巴细胞预防和治疗人肝癌裸鼠皮下移植瘤。

The soluble protein content and its SDS-PAGE patterns in leaves of Neosinocalamus affinis, Dendrocalamus farinosus and Bambusa rigida from the different regions of Sichuan province were studied.

以四川省不同地区慈竹、梁山慈竹及硬头黄竹的叶片为材料，测定叶片可溶性蛋白质含量，采用SDS-PAGE技术研究其可溶性蛋白谱带的差异。

It's almost 10 years since Page and Brin launched their search engine.

这是近10年以来， Page和Brin推出他们的搜索引擎。

That all changed in 1998 when Page and Brin founded Google.

这一切在1998年，Page和Brin创办Google时发生了变化。

Company founders Larry Page and Sergey Brin found out that he was an art major in college.

公司创始人Larry Page和Sergey Brin才发现他原来在学校学的是艺术专业。

By Using SDS-PAGE gel electrophoresis inorder to study the expression of crystalline in lens regeneration of Bufo Raddei Strauch Tadpole.

用SDS-PAGE电泳方法研究再生过程中晶状体蛋白的表达。

The caprine IL-2 gene and the IFN-γgene without the signal peptidesequence were subcloned into (5") Bam H I and (3") HindⅢrestriction sites of pET30aplasmid, the recombinant plasmid were transformed into E. coli BL21(DE3) and induced withIPTG, they were demonstrated by SDS-PAGE that the genes were expressed as inclusion bodies.

应用DNA重组技术，将IL-2基因和去除信号肽的IFN-γ基因分别插入到原核表达载体pET-30a的BamHⅠ和HindⅢ位点上，构建原核表达质粒pET30a-CapIL-2和pET30a-CapIFN-γ，转化大肠杆菌BL21(DE3)，经IPTG诱导后进行SDS-PAGE电泳，结果表明目的基因在大肠杆菌中以包涵体形式融合表达。

The main results are as follows: 1 Development of colloidal gold immunochromatographic assay for rapid detection of AFB_1 (1) One ascitic IgG monoclonal antibody was purificated by caprylic acid-ammonium sulfate precipitation.Activities and purity of monoclonal antibody were monitored by indirect ELISA and SDS-PAGE, respectively.

本论文研究的内容主要包括以下两个方面： 1 AFB_1胶体金免疫层析快速检测方法的研究(1)利用辛酸-硫酸铵盐析法纯化小鼠腹水中抗AFB_1单克隆抗体，分别采取间接非竞争ELISA和SDS-PAGE对纯化后抗体的活性和纯度进行鉴定。

Methods: Biological technique, including centrifugalization, ion-exchange chromatography, hydrophobic chromatography and dialysis was used. Osteopontin from bovine milk were identified by SDS-PAGE and Western Blot.

运用生物化学技术，通过离心脱脂后，再经过离子交换层析、疏水层析、透析等技术达到分离纯化目的；随后对分离纯化得到的产物进行SDS-PAGE电泳、免疫印迹技术鉴定。

I will endeavour to find you some assistance.

我尽力帮你找人帮忙。

At first I only know bruck is the idol of American younglings, afterwards I returned back to Taiwan ,even in Beijing last year ,I saw her poster everywhere, I was so surprised at her charm.

起初我只晓得布鲁克雷德丝是美国少男少女崇拜的偶像，后来回台湾，甚至去年在北京，居然也四处看见她的海报，才惊讶她的魅力之大。