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Those expression plasmid were respectively transformed into E.coli DH5a, thenthe expression strains were induced for 5h under 42℃, SDS-PAGE confirmed that interestprotein was expressed by pBV220-R- IFN-γ_m strain and was 16kd in size. Its contentaccounted for 38.6％of the thalline protein.

分别转化大肠杆菌DH5α，42℃诱导表达4h，经SDS-PAGE电泳鉴定，原核表达质粒pBV220-R-IFN-γ_m表达的目的蛋白大小为16Kd，占细菌总蛋白的38.6％。

The pI of these components gradually decreased f rom pH 4.0 according to electrophoresis mobility from higher to lower on PAGE. The molecular weights were in the range of 22～34 ku. 6.5 and 7 w ere glycoproteins proved by staining with the shiff reagent and thymol/sulfuric acid. The fibrinolytic activity of 7 was highest as determined usi ng chromzym UK and chromzym PL as specific substrates.

这些组分的等电点按照它们在聚丙烯酰胺凝胶电泳图谱上的顺序从4.0开始依次降低；SDS-PAGE证明，除3、4外，其余组分均只含一种多肽链，分子质量在22～34ku之间；用shiff试剂和酚-硫酸染色，显示1、2、6.5和7是糖蛋白，其中7的糖含量最高；以BAEE、Chromozym UK和Chromozym PL为底物测定，7的纤溶酶活性最高。

In this dissertation, firstly, the conditions of isolation and purification of specific toxin fractions produced by Exserohilum turcicum has been studied, and a high-efficient method has been established;secondly, the toxins were analysed by UV-Vis spectrophotometry;then the effect of specific toxin on death rate of corn leaves protoplast was studied by FDA as a tinct reagent;finally, using ANS as a probe and SDS-PAGE to study the membrane protein of the corn leaves with Htl gene. In a word, the aim of the research is to search some existent evidences of the toxin binding site on protoplasmic membrane, and provide proofs for nosogenesis of specific toxin in terms of cytology and the molecular biology.

本研究对玉米大斑病菌1号小种毒素的特异性组分进行分离，建立了高效的特异性毒素分离纯化方法，并对其进行了紫外波谱分析；用荧光素双醋酸酯染色法，测定了毒素特异性组分对原生质体死亡率的影响；进而用1-苯胺基-8-萘磺酸荧光探针标记法和SDS-PAGE电泳法对特异性毒素组分与Htl基因玉米细胞互作过程中的质膜蛋白进行了研究，旨在证明Htl基因玉米细胞质膜上存在特异性毒素组分结合蛋白，为从细胞和分子生物学角度阐明毒素的致病机理提供证据。

Total anthers proteins were extracted from uninucleate and binucleate stage of-1376A and-1376B for IEF/SDS-PAGE.An higher repartability and better separatingprotein method from wheat anther for 2-DE was established.

以IEF／SDS-PAGE双向凝胶电泳技术，对小麦质核互作型雄性不育系-1376A及其保持系-1376B在花药发育的单核期、二核期蛋白质进行了差异蛋白质组学研究。

Urea-SDS-PAGE showed that no unpurposed propein was found after ConA-sepharose chromatography.2. Identification of alliinaseⅠ, alliinaseⅡ Two different alliinase, named alliinaseⅠand alliinaseⅡ, were found in ConA-sepharose chromatography.

尿-SDS-PAGE电泳图显示，经亲和层析纯化后的酶无杂蛋白带。2、酶Ⅰ、酶Ⅱ的鉴定在ConA-Sepharose亲和层析操作中，发现大蒜鳞茎中存在两种不同的蒜氨酸酶：命名为酶Ⅰ、酶Ⅱ，其中酶Ⅱ是主要部分，酶Ⅰ活性较弱。

Fourth, we constructed pBES-CFA/I vectorial vaccine and test the expression of CFA/I by SDS-PAGE.

第四、构建了pBEGFP-CFA/I 载体疫苗表达质粒，并进行了SDS-PAGE检测。

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Using ammonium sulfate precipitation, Blue Sepharose CL-6B psuedo-ligand affinitive chromatography and Sephadex G-75 gel chromatography, we obtained the typeⅠRIP with high purity, the feasibility of this method has been approved by SDS-PAGE results.

结果显示：1。运用硫酸铵分级沉淀、Blue Sepharose CL-6B假配基亲和层析、Sephadex-G75凝胶层析等方法提取、纯化得到了高纯度的苦瓜RIP，并且本方法提取效率较高，所获得的RIP在SDS-PAGE上呈现为单一条带。

In order to investigate the quality of wheat in Huanghuai southern regions, 36 wheat cultivars were fractionated by SDS-PAGE to determine their HMW-GS composition, the variation of the subunits from different allelomorphic genes and the frequency were also analyzed.

为了解黄淮麦区小麦的品质状况，应用SDS—PAGE方法对黄淮麦区推广小麦品种的高分子量麦谷蛋白亚基组成、变异厦出现频率进行了分析，并计算了参试品种的品质得分。

The 75kD polypeptide in SDS-PAGE about all protein in eight isonuclear alloplasmic and allonuclear isonuclear male-sterile lines had been found keeping close with the male sterile.

对8 个同核异质、同质异核不育系和相应保持系进行花药全蛋白SDS-PAGE，发现了75kD多肽与雄性不育关系密切。

I will endeavour to find you some assistance.

我尽力帮你找人帮忙。

At first I only know bruck is the idol of American younglings, afterwards I returned back to Taiwan ,even in Beijing last year ,I saw her poster everywhere, I was so surprised at her charm.

起初我只晓得布鲁克雷德丝是美国少男少女崇拜的偶像，后来回台湾，甚至去年在北京，居然也四处看见她的海报，才惊讶她的魅力之大。