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During the shift from the mechanical to digital, the tools of graphic design production—page makeup and imaging software, typefaces, and so on—became freely available and relatively cheap.

在期间从机械手指，工具的平面造型设计转移可用的production—page构成和造像软件，字面和那样 on—became自由和相对说来便宜。

Brin, who had met Page at a student orientation a year earlier, joined the project early on.

Brin是在95的一次学生联谊上结识Page的，并在早期就参于了项目的研究。

While on the track, press the "PAGE UP" key, and use the NumPad keys to rotate around the car.

当你在赛道上时，按"PAGE UP"键。然后使用小键盘上的数字键可以围绕着车子旋转。

RESULTS A eukaryotic expression system for high expression humanmutantCD59 were successfully set up : The recombinant PALTER-MAX plasmid containing human mutantCD59 cDNA and PCDNA plasmid were co-transfected into CHO cell by cation lipoid mediating method ;and the cells were grown in F12 medium containing 400ug/ml G418 for 14 days, positive clones were grown in RPMI1640 medium to get stable expressing cell lines . Highly expressing clones were selected by flow cytometry ,and were named PALTER-CD59-CHO1PALTER-CD59-CHO2 . Flow cytometry indicated that expression rates of PALTER-CD59-CHO1 and PALTER-CD59-CHO2 were 53.7%and 54.5%. Further more, Stable highly expressing CHO cell lines were more detected by immunocytochemistry and immunofluorescence technology . PALTER-CD59 -CHO1 and PALTER-CD59-CHO2 were grown in RPMI1640 to get a large of cells . CD59 protein were obtained by spalling PALTER- CD59- CHO1 and PALTER - CD59 - CHO2 cells . Stable highly expressing cells were further validated by SDS-PAGE, immunoblot analysis and solid enzyme immunoassay . PALTER - CD59 - CHO1 and PALTER - CD59 - CHO2 were glycated in RPMI1640 of 50mM ribose for 72 hours .BCECF releasing test indicted that the releasing rate of PALTER-CD59-CHO1 or PALTER-CD59-CHO2 was less high than PALTER-CHO ,and the releasing rate of glycated PALTER-CD59 -CHOI or PALTER-CD59-CHO2 was higher than unglycated ones . PALTER -CD59-CHO1 and PALTER -CD59 -CHO2 were glycated in RPMI1640 of 50mM ribose for 72 hours .BCECF releasing test indicted that the releasing rate of PALTER - CD59 - CHOI or PALTER - CD59 - CHO2 was less high than PALTER-CHO ,and the releasing rate of glycated PALTER-CD59-CHO1 or PALTER - CD5 9-CHO2 was higher than unglycated ones .

结果 成功构建突变人CD59的真核细胞表达系统：运用阳离子脂质体介导法将含有突变人CD59的PALTER—MAX重组质粒与PCDNA共转染入CHO细胞：用含有400ug／mlG418的F12培养基培养14天，筛选出稳定阳性表达克隆，RPMI1640培养基扩增获得稳定表达细胞株，并用流式细胞术进一步筛选出高效表达细胞株分别命名为PALTER—CD59—CH01、PALTER—CD59—CH02，表达率分别为53.7％、54.5％；应用免疫组化方法、免疫荧光技术进一步鉴定阳性细胞株；RPMI1640培养基大量扩增PALTER—CD59—CH01、PALTER—CD59—CH02细胞株，裂解细胞得到CD59蛋白质；通过SDS—PAGE凝胶电泳技术、免疫印迹技术、固相酶联免疫吸附试验验证了这两中文摘要个阳性细胞株CO59蛋白的高效表达；50mM核糖培养72小时，获得突变人CD59糖化细胞株，BCECF染料释放试验结果显示，PALTER一CD59一CHOI、pALTER一CD59一CHOZ细胞较PALTER一CHO细胞染料释放率低，未糖化PALTER一CD59一CHOI、PALTER一CD59一CHOZ细胞比较糖化后细胞染料释放率低。

The changes of nutrition in milk treated with different technologies are also determined. Results showed that, 1 higher pressure, longer holding time and more alternative times favour a larger destruction of microorganisms; 2 the combined effect of hurdle technology and pressure on the destruction of microorganisms in fresh raw milk is more effective than only using pressure treatment, among which the effect of ε-polylysine on inactivation of microorganism in fresh raw milk is more effective than that of Nisin with the same concentration of 100mg/L; 3 the whole proteins in milk treated by the different types of sterilization have been detected by SDS-PAGE electrophoresis, and comparing with the whole proteins' electrophoretogram of fresh raw milk, we found that the first spectrum band near the negative pole was disappeared and the colours of the second spectrum band was tint in UHT, pasteurism and 500MPa treated milks, which was suggested that some proteins' subunits were dissociated, but the 400MPa treated milk showed the similar eletrophoretogram with the fresh raw milk; 4 the loss of threonine, VA and VC in high pressure treated milk was more less than those in pasteurism treated milk, in particular threonine and VA were almost retained, and the contents of VB1 and VB2 in the former are also more than that in the latter; 5 high pressure treated milk had longer shelf life than pasteurism treated milk, extending the sale semidiameter of milk.

压力越高，保压时间越长，交变次数越多，杀菌效果越好；2）采用栅栏技术对牛乳中的微生物具有更有效的杀菌作用，其中相同浓度的（终浓度100mg/L）ε-聚赖氨酸较Nisin具有更强的杀菌效果；3）利用SDS-PAGE电泳研究了不同杀菌方式对牛乳总蛋白的影响，发现经UHT、巴氏和500MPa交变处理的牛乳与生鲜牛乳相比其总蛋白电泳图谱靠近负极方向第一条谱带消失，第二条谱带颜色变浅，由此推测经上述处理后牛乳中有一些蛋白质的亚基发生了解离，而经400MPa交变处理的牛乳与生鲜牛乳的总蛋白电泳图谱相似；4）超高压牛乳营养成分与生鲜牛乳相比，苏氨酸、VA、VC在超高压处理中受到的破坏明显比巴氏杀菌小得多，特别是苏氨酸和VA几乎完全被保留下来，而VC含量也要比巴氏奶高出25%以上，对于VB1、VB2，检测数据也表明，超高压灭菌对其破坏要比巴氏灭菌小，其含量分别比巴氏杀菌牛乳高出9.5%和2.6%；5）与巴氏杀菌乳相比，超高压杀菌有助于延长牛乳的保质期，扩大牛乳的销售半径。

SDS-PAGE analysis revealed that with IPTG induction, the recombinant strain harboring the plasmid pPel9A could express the target protein (130 000), with the molecular weight same as the expected pectate lyase protein.

重组菌经IPTG诱导和SDS-PAGE分析表达的蛋白质的相对分子质量为130 000，与预期相对分子质量相符。

According to Page's tensile strength equation,we deduce that the decrease of shear strength of recycled fibers is the major cause of quality decay of recycled fibers when considering the change of pentosan content,the change of fiber length distribution and the change of scattering coefficient of paper.

根据著名的Page方程，从回用纤维中聚戊糖含量的变化和回用纤维长度分布的变化以及回用纤维纸页光散射系数变化等数据中推断出了影响回用纤维品质衰变的主要因素，并进一步探讨了这些因素影响回用纤维品质的机

The Trypsin inhibitor from Cucurbita pepo var. striaca Seeds was isolated by salting-out and chromatography of Sephadex G-50, the inhibitor activity was detected with BAPNA, the Mw was determined with SDS-PAGE and stablity was studied with different temperature, the side chains of Lys and Cys were modified.

采用分级盐析和Sephadex G-50的方法分离纯化无种皮南瓜籽胰蛋白酶抑制剂，用BAPNA法进行抑制活性的测定，用SDS-PAGE法测定蛋白质的分子量，用不同的温度处理方法对它的热稳定性进行研究，并对抑制剂的Lys和cys进行了化学修饰。

The results of SDS-PAGE and differential displays both showed that the composition of cell proteins and the expression of nuclear genes were changed after the loss of plastid.

SDS-PAGE与选择性cDNA 3'端片段的差异显示结果都表明，失去质体之后，细胞的蛋白质组成和细胞核的基因表达发生了改变。

SDS-PAGE results showed that as to Mut+ recombinant highest yield was obtained after 4 days inducing and with the culturetime prolonged it reduced. Pokeweed antiviral protein gene expressed well when methanol concentration reached 10g/L. Pokeweed antiviral protein obtained high yield in thin acidic culture medium (pH6.0-6.4) and its quantity in total mass of secrete protein exceeded 30%.

SDS-PAGE分析结果表明，Mut~+组菌株在甲醇诱导第四天后PAP在培养液中积累量达到最高水平，延长培养时间会导致产量下降；在10g/L的甲醇浓度诱导下，PAP的表达量达到最高；培养基pH值在偏酸性条件下(6.0-6.4)PAP的表达量都维持在较高的水平。

However, as the name（read－only memory）implies, CD disks cannot be written onorchanged in any way.

然而，正如其名字所指出的那样，CD盘不能写，也不能用任何方式改变其内容。

Galvanizes steel pallet is mainly export which suits standard packing of European Union, the North America. galvanizes steel pallet is suitable to heavy rack. Pallet surface can design plate type, corrugated and the gap form, satisfies the different requirements.

镀锌钢托盘多用于出口，替代木托盘，免薰蒸，符合欧盟、北美各国对出口货物包装材料的法令要求；喷涂钢托盘适用于重载上货架之用，托盘表面根据需要制作成平板状、波纹状及间隔形式，满足不同的使用要求。