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Pentosaceus. These four bacteriocins showed the highest antibacterial activity under acidulous and neutral conditions. The bacteriocin-producing strains inhibited most of related lactic acid bacteria. The bacteriocins produced by strains L. acidophilus Ind-1 and P.

戊糖片球菌产生的细菌素对木瓜蛋白酶敏感,而其它三种细菌素对这种蛋白酶稳定。pH稳定性研究表明,在弱酸性或中性条件下,这些细菌素表现出较高的活性。

Results Six compounds were isolated and identified as:5α,8α-epidioxy-(22E,24R)-ergosta-6,22-dien-3β-ol,scopoletin,isofraxidin,p-hydroxy benzaldehyde,3,4,5-trimethoxy benzoic acid,protocatechuic acid ethyl ester...

结果得到6个化合物,分别鉴定为:5α,8α表二氧(22E,24R)麦角甾6,22二烯3β醇、东莨菪素、异秦皮啶、对羟基苯甲醛、3,4,5三甲氧基苯甲酸、原儿茶酸乙酯。结论化合物Ⅰ~Ⅵ均为首次从该属植物中分离得到。

Synthesis of perfluoroalkylethylacrylate from polyfluoroalcohol and acrylic acid with p toluene sulphonic acid as catalyst was studied.

以对甲苯磺酸为催化剂,多氟醇和丙烯酸为原料合成全氟烷基乙基丙烯酸酯。

0 H; reaction temperature: 140~ 150°C ; molar ratio of p toluene sulphonic acid to salicylic acid, 1∶11, with no water carrying reagent; yield: about 98%.

应用对甲苯磺酸作为水杨酸和 2 -乙基己醇的酯化催化剂,成功合成了水杨酸- 2 -乙基己酯。

Tetradecyl acrylate was synthesized by direct-etherification from tetradecyl alcohol and acrylic acid with p-methyl benzene sulfuric acid as catalyst.

摘 要:以丙烯酸和十四醇为原料,经酯化反应直接合成丙烯酸十四酯。

The side chain of thioacetic acid has been sythesized from the material of acetic acid via four steps, then synthesized the key intermediate of 2-hydroxypenem via displacement, P〓induced cyclization and hydrolyzed reaction from the material of azetidinone.

以羟乙酸为原料,经四步反应合成了侧链化合物硫基取代的羟乙酸,发生取代反应导入到经七步合成的单环氮杂环丁酮中,然后发生酰化,Wittig诱导环化,水解等五步反应合成关键中间体2-羟甲基青霉烯。

The result showed that the contents of p hydroxy benzoic acid,vanillic acid(extracted by 1mol·L -1 NaOH) in continuous cropping soil were significant higher than that in normal cropping soil,and the content of vanillin had no significant difference in these soils.

结果表明,重茬土壤中对羟基苯甲酸和香草酸的含量(1mol·L-1NaOH提取)大于正茬土壤,且差异达到极显著水平,香草醛含量差异不显著;重茬大豆根系水提液中对羟基苯甲酸、香草酸、阿魏酸、香草醛、香豆素含量均高于正茬。

The detected phenolic, gallic acid was gradually increased with continuous cropping years increasing, vanillic was steady in soil of different continupus cropping years, ferulic acids had more content in soil of five-year continuous cotton cropping, but remarkably reduced with ten-year continuous cropping.7 It was found that the p-hydrobenzoic, vanillic, gallic acid and ferulic acids in different comentration inhibited/stimulated the spore germination and mycelium growth of cotton verticillium wilt.

没食子酸含量随着连作年限增加在土壤中逐渐增加,香草醛在不同连作年限土壤中含量较稳定,而阿魏酸连作5年土壤中含量最高,连作10年时显著下降。7不同浓度的对-羟基苯甲酸、阿魏酸、香草醛和没食子酸对棉花黄萎病病菌孢子萌发和菌丝生长产生促进或抑制的化感作用。

By use of site mutation strategy and PCR technology, we obtained the gene P12X3C that includes full length PI, 2A, 3C and a part of 2B and 3B and the gene P12X3C3D that includes full length PI, 2A, 3C, 3D and a part of 2B and 3B. After being digested by restriction enzyme respectively, the gene P12X3C and the gene P12X3C3D were cloned into the pcDNA3.1 and pTARGET expression vector that were digested by the same enzyme. Recombinant plasmids were checked by restriction enzyme analysis and nucleic acid sequencing. Further more, recombinant plasmids were transfected into BHK-21 cells by using lipoid. The proteins of foot-and-mouth disease virus, which were expressed in BHK-21 cells, were confirmed by sandwich-ELlSA and fluoroscopy, and the capsid of FMDV was tested by electron microscope. In order to evaluate enhanced immune response of guinea pigs against FMDV, DNA vaccines which were designed to produce viral capsids lacking infectious viral nucleic acid and contained the gene P12X3C and the gene P 12X3C3D were injected respectively with FMDV 3D protein which was expressed in Pichia Pastoris Secreted expression System and purified or with pcDNA3.1/lFN which includes the gene IFN-a of cattle. Subsequently, Recombinant plasmids were injected to catties with or without pcDNA3.1/IFN. Anti-FMDV antibodies were detected by ELISA, and the T lymphocyte proliferation response was tested by MTT assay, neutralization antibodies liters were analyzed by micro-neutralization assay.

为研制带有O型口蹄疫病毒(Foot-and-Mouth Disease Virus,FMDV)China99株结构蛋白基因及多个非结构蛋白基因的DNA疫曲,本研究通过定点突变方法和PCR扩增方法,获得包含有FMDV China99株结构蛋白P1、非结构蛋白2A、3C以及部分2B、3B编码基因的片段P12X3C和包含有FMDV China99株结构蛋白P1、非结构蛋白2A、3C、3D以及部分2B、3B编码基因的片段P12X3C3D,将获得的基因片段直接/酶切后与同样处理的真核表达质粒连接,分别得到重组质粒pcDNA3.1/P12X3C和pcDNA3.1/P12X3C3D、pTARGET/P12X3C3D;对重组质粒进行序列测定、分析,并将重组质粒分别转染BHK-21细胞,通过双抗体夹心ELISA方法和间接免疫荧光标记方法检测细胞中FMDV抗原的表达,用电子显微镜观察病毒空衣壳的组装;为评价重组质粒作为DNA疫苗对实验动物及本动物的免疫效果,将重组质粒经肌肉注射方法接种豚鼠,并与酵母表达的纯化FMDV China99株3D蛋白及带有牛α干扰素的真核表达质粒pcDNA3.1/IFN分别/同时免疫,第二次免疫后第三周豚鼠攻以100ID_(50)或1000ID_(50)的O型FMDV China99株:随后将质粒pcDNA3.1/P12X3C、pcDNA3.1/P12X3C3D与带有牛α干扰素的真核表达质粒pcDNA3.1/IFN同时免疫牛,三周后经牛舌皮攻以10~4ID_(50)的O型FMDV China99株。

O date, the latter has been identified in several organs and tissues such as pancreatic p-cell, the liver, adrenal cortex and skeletal muscle where they regulate p-cell survival, hepatic glyconeogenesis, steroid synthesis, and mitochondrial biogenesis and fatty acid oxidation in the skeletal muscle.

ORC-CREB复合体广泛存在于多种器官与组织,如胰岛β-细胞、肝脏、肾上腺皮质和骨骼肌中,与胰岛β-细胞存活、肝脏糖异生、类固醇激素合成、骨骼肌线粒体增生与脂肪酸β氧化密切相关。

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This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。

There is no differences of cell proliferation vitality between labeled and unlabeled NSCs.

双标记神经干细胞的增殖、分化活力与未标记神经干细胞相比无改变。