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objective to study gastrodia elata blume to effect tau protein,sod,mda expression in gyrus hippocampi and cerebral cortex of experimental mice.methods to inject okadaic acid to mice ventriculus lateralis and to measure tau protein level,sod activity and lipid superoxide mda content of sod control group,oa injection group,oa and gastiodia elata injection solution group.results tau protein of experimental group(p<0.05/p>0.05),sod was lower than model group(p<0.001) and was higher than control group(p<0.05).conclusion gastrodia elata blume can increase sod activity and reduce tau protein expression and superoxide lipid forming in brain tissue of experimental dementia mice caused by oa.it can prevent and treat ad.

目的 观察天麻对痴呆模型大鼠海马、皮质神经元微管相关蛋白、超氧化物歧化酶和脂质过氧化物丙二醛表达的影响,探讨其治疗阿尔茨海默病(alzheimer disease,ad)的作用机制。方法用冈田酸(okadaic acid,oa)注射大鼠侧脑室造模,测定模型组、实验组、对照组海马和皮质tau蛋白、sod、mda的含量。结果实验组tau蛋白低于模型组(p<0.001),高于对照组(p<0.05);sod高于模型组(p<0.001)和对照组(p<0.05);mda低于模型组(p<0.001),高于对照组(p<0.05)。结论天麻可增强oa致实验性痴呆大鼠脑组织sod活性,降低mda蓄积和tau蛋白生成,具有防治阿尔茨海默病的作用。

The p-coumaric acid content in rice straw and pig feces was higher than frulic acid and the p-hydroxybenzoic acid content in wood chip was higher than p-coumaric acid.

稻草、猪粪在腐解过程中香豆酸含量最高,阿魏酸次之,木屑中对羟基苯甲酸最高,香豆酸次之。

RESULTS:In the case of ambient temperature cefoxitin with etamsylate is in compatibility steady-state both in compatible content and outward stability (P>0.05) within 6h;cefoxitin with aminomethylbenzoic acid or adrenochromazone is in compatibility steady-state (P>0.05) within 4h;cefoxitin with aminocaproic acid varies in compatible content (P<0.05) after 4h.

结果:在室温条件下,头孢西丁与酚磺乙胺在0~6 h内含量(P>0.05)和外观稳定,但pH值有变化(P<0.05);与氨甲苯酸、安络血在0~4 h内稳定(P>0.05);与氨基己酸配伍有变化(4 h后P<0.05)。

The experiment two: enzyme preparation significantly improved average daily gainand feed conversion ratio (P<0.05). Enzyme preparation significantly increased energymetabolizability and digestibility of crude fiber, crude protein and neutral detergent fiber,but had no remarkable effect on digestibility of dry matter, crude fat and acid detergentfiber. Enzyme preparation significantly decreased the relative viscosity of duodenal andjejunal digesta. The pH of intestine had no noticed difference in all groups. Enzymepreparation significantly decreased relative weight of gizzard, proventficulus, duodenum,jejunum and ileum. Enzyme preparation significantly increased villus size of duodenumand jejunum, and villus to crypt ratio of duodenum and ileum significantly increased too.Enzyme preparation considerably decreased ileal crypt height (P<0.05), and didn"t affectthickness of intestinal wall. Supplementing enzyme preparation, the serum glucose, totalprotein and alanine aminotransferase, but enzyme preparation hadn"t noticed influenceupon uric acid, total cholesterol, triglyceride and high-density lipoproteins. Enzymepreparation significantly increased insulin, triiodothyronine and insulin-like growthfactor-Ⅰ. Adding enzyme preparation, the percentage of thyroid stimulating hormone andgrowth hormone in the serum increased 16.44%, 19.18% and 18.84%, 21.74%respectively, and the percentage of glucagon and thyroxine decreased 12.07%, 14.36% and 13.79%, 15.40%, but failed to reach statistical significance (P>0.05). Enzymepreparation significantly increased (P<0.05) the trypsin and amylase activity of duodenaland jejunal digesta, but enzyme preparation didnt affect significantly (P>0.05) theintestinal lipase activity and pancreatic digestive enzyme. Enzyme preparation had nosignificant effect on caecal microbial population.

试验二:酶制剂显著提高平均日增重和饲料转化率(P<0.05);酶制剂显著提高能量代谢率及粗纤维、粗蛋白、中性洗涤纤维消化率(P<0.05),而对干物质、粗脂肪、酸性洗涤纤维消化率影响不显著;酶制剂显著降低十二指肠和空肠食糜相对粘度(P<0.05);添加酶制剂对肠道pH影响不显著;酶制剂显著降低肌胃、腺胃、十二指肠、空肠、回肠相对重(P<0.05),显著提高十二指肠和空肠绒毛高度,显著增加十二指肠和回肠绒毛高度/隐窝深度,降低回肠隐窝深度(P<0.05),对肠壁厚度影响不显著;酶制剂显著提高血清葡萄糖、总蛋白和谷丙转氨酶浓度(P<0.05),对尿酸、总胆固醇、甘油三酯及高密度脂蛋白浓度影响不显著,显著提高胰岛素、T_3、IGF-Ⅰ水平,添加酶制剂后,促甲状腺激素、生长激素分别提高16.44%、19.18%和18.84%、21.74%,胰高血糖素和T_4分别降低12.07%、14.36%和13.79%、15.40%,但差异不显著;酶制剂对胰腺消化酶活性影响不显著,显著增加十二指肠和空肠胰蛋白酶、淀粉酶活性,对小肠脂肪酶活性影响不显著;酶制剂对盲肠微生物菌落数影响不显著。

The reaction of 4-bromaophenol and geranic acid in the presecnce of p-TsOH afforded 4-bromophenolα-cyclogeranoate in which cyclization of geranic acid toα-cyclogeranic acid was followed by esterification ofα-cyclogeranic acid with p-bromophenol.

对溴苯酚与香叶酸在p-TsOH催化作用下发生了香叶酸向α-环香叶酸环化、α-环香叶酸环与对溴苯酚的酯化,得到了唯一产物α-环香叶酸对溴苯酯,产率68%。

In the adipose tissue of obese pigs,the percentagesof myristic acid(C14:0),linoleic(C18:2)and linolenic acid(C18:3)were lower(P<0.01),and that of palmitate(C16:0)and stearic acid(C18:0)were higher(P<0.01),the percentages of the total SFA washigher,and that of total UFA and total PUFA were lower,than thatof lean lean pigs respectively.

肥胖型猪脂肪中豆蔻酸、亚油酸和亚麻酸含量较低(P<0.01),而棕榈酸(C16:0)和硬脂酸(C18:0)含量则较高。含量最多的油酸差异不明显。从各类脂肪酸总量来看,脂肪型猪的SFA含量较高,而UFA和PUFA含量较低(P<0.01)。尤其是PUFA的差异最为明显。并进一步探讨了这种差异对脂肪最终品质的影响。

The content of essential amino acid and flavor amino acid of experiment groups were higher than control group, especially group 1.0% small peptide were increased by 6.4% and 2.5%; IMP content of experiment groups was increased in different extent, especially group 1.0% small peptide was 0.17mg/100mg and increased by 70.0%(P.05); The content of palmitoleic acid、 octadeca、 arachidonic、 arachidic and stearic acid of experiment groups were higher than control group indifferent extent.

随着小肽用量的增加,成鲤肌肉中的总氨基酸含量呈上升趋势,必须氨基酸和风味氨基酸的含量也随之提高,尤其是1.0%小肽组的必须氨基酸和风味氨基酸的含量分别比对照组提高6.4%和2.5%,其中谷氨酸的含量最高,为10.70mg/100mg,比对照组提高14.0%(P<0.05);随着饵料中小肽用量的增加,成鲤肌肉的肌苷酸含量显著提高(P<0.05),1.0%小肽组的IMP含量高达0.17mg/mg,比对照组提高70.0%;成鲤肌肉中的油酸和二十?

In order to check the corresponding compound at 31P NMR –45.4 ppm, stable pentacoordinate spirobicyclic 2-phenoxy-1,3-phenylene-dioxo-1,3,2-iminoacetoxyphosphoranes were synthesized through a new and efficient method whereby phosphorus pentachloride was displaced stepwise by catechol, N,O-bisamino acid and phenol, or catechol, phenol and N,O-bisamino acid, their 31P NMR chemical shifts are at about 31P NMR –45.0 ppm, the results showed that the compound 4 at 31P NMR –45.4 ppm was pentacoordinate phosphorane containing amino acid residue.

采用磷谱跟踪来研究上述成肽反应机理。例如在氮气保护下,0.1mmol O,O-亚苯基磷酰氯(1)和0.15mmol N,O-二苯丙氨酸(2)苯溶液加入0.6ml核磁管中,混合均匀,反应过程用磷谱检测。反应1分钟后,起始原料1(δp=19ppm)很快转变成N-磷酰苯丙氨酸的三甲基硅基酯(3)(δp=19.8ppm),在8分钟内,3完全转变成苯丙氨酸磷烷4(δp =-45.4ppm),同时出现环磷三酯(6)(δp=3.5ppm)。随着反应的进行,对应于化合物4的峰逐渐变小,而6逐渐变大。

C. Organic intermediate products: P-Methylbenzonltrile, P-Bromobenzoin acid, 3,5-Dimethylbenzoic acid, P-Ethylbenzoic acid, 1,3,5-Benzene Tricarboxylic acid, 1,4-Naphthalic acid.

C。有机中间体产品:对甲苯腈。对溴苯甲酸、3.5-二甲基苯甲酸、对乙基苯甲酸、均三苯甲酸、KCB酸。

The results showed that the two kinds of Ni-P electroless deposits are both amorphous. The phosphorus content in the deposit using lactic acid as complexing agent is 10.04%, and in the deposit using citric acid as complexing agent is 11.93%. As the Na2S content increases, the polarizability increases, and the corrosion current and corrosion potential decrease while the Na2S content is between 1.0%~2.5%. The corrosion resistance of Ni-P electroless deposit using lactic acid as complexing agent is better than that of another kind.

结果表明,利用两种络合剂形成的Ni-P镀层均为非晶态,其中以乳酸为络合剂的镀层中P含量为l0.04mass%,以柠檬酸为络合剂的镀层中P含量为11.93mass%,在1.0%~2.5% Na2S多介质中,随着Na2S多含量的增加,阳极极化曲线的极化率增大,自腐蚀电位和自腐蚀电流降低。

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