查询词典 overproduced

1 .he serum cTnT and the serum cTnI levels are overproduced in congestive heart failure patients, that of the third degree heart failure is the highest,the following is the second degree heart failure groud.

1。充血性心力衰竭病人血清cTnT和cTnI浓度均明显升高，升高程度依次为Ⅲ度心力衰竭病人最大，Ⅱ度心力衰竭病人次之，Ⅰ度心力衰竭病人最小。

Escherichia coli leucyl-tRNA synthetase was overproduced and purified from an overexpression strain.

采用含pTrc-99B/leuS2转化子的高表达菌株，简便快速地提取、纯化大肠杆菌亮氨酰-tRNA合成酶。

Surgical manipulation can induce inflammatory response within small intestine, stimulate intestinal smooth muscle cells to express iNOS mRNA. As a result, the overproduced NO inhibited the small intestinal motility.

手术操作诱发了小肠肌层炎症反应，并刺激小肠平滑肌细胞表达iNOS，过度产生NO，抑制小肠环行平滑肌的收缩功能。

Also stirring is the anthem"When you believe"—best sung in character by Michelle Pfeiffer and Sally Dworsky(a far superior rendition than the overproduced hit single by Whitney Houston and Mariah Carey).

还有让人迷醉的颂歌《当你相信》——米雪菲花和莎莉凯丽的角色唱比惠特尼休斯顿和玛利亚凯丽满天飞的金曲要优秀的多。

Coli JM109 (DE3) transformant containing the 〓-tagging vector with the gene leuS was approximately 110 times that of JM109 (DE3)(the host strain without the vector). The overproduced 〓-fusion leucyl-tRNA synthetase can be purified to homogeneity under native condition within two hours by one step affinity chromatography with an overall yield of 55%.

高表达的N—端带有6个连续组氨酸的LeuRS经过一步Ni-NTASuperflow亲和层析可以达到90％以上的纯度，产率为55％，纯化时间仅为2小时，而且LeuRS所带的〓-标签对酶的结构和功能都没有影响。

Overproduced YlyA existed as inclusion body, while the induction was carried out with 0.5mM IPTG, at 37℃ for 3h; Modified the induction condition to 0.05mM IPTG, at 25℃ for 5h, a portion of His-tagged YlyA turned into soluble; After purification treatment, the concentration of the fused protein reached to 204.2119μM.

结果表明pNG252中ylyA的插入方向正确，序列无突变；用0.5mM IPTG ，37℃诱导3h时，YlyA虽高效表达，但为包涵体形式；调整诱导条件至0.05mMIPTG，25℃，5h时，高效表达的YlyA部分转为可溶性蛋白。

YlyA sequence was amplified by PCR and cloned into pETMCSIII, to give pNG252. 6×His-YlyA was induced to express in BL21, overproduced YlyA was checked for its solubility and purified by Ni2+-NTA affinity chromatography. The result suggested that both the sequence and the direction of inserted fragment ylyA were correct.

PCR扩增ylyA序列，将其克隆到pETMCSIII中构建表达载体pNG252，用IPTG诱导6×His-YlyA融合蛋白在大肠杆菌BL21（DE3）中表达，对表达产物进行分析，最后对可溶性YlyA重组蛋白进行Ni2+-NTA亲和层析加以纯化。

The chromosomally located cephalosporinase and carbapenem-hydrolyzing beta-lactamase NmcA were inducible by cefoxitin and meropenem in E. cloacae NOR-1, and both were stably overproduced in the ceftazidime-resistant mutant.

在E阴沟杆菌NOR-1中，位于头孢菌素酶和氨基甲酰水解的β-内酰胺酶NmcA中的染色体可以被头孢西丁和美罗培南诱发，而且两者在头孢三嗪噻肟抗体突变体中都是稳定的大量产生的。

Here, we demonstrated in this work that CsrA performs a negative regulator of the cel expression. cel, encoding lysis protein (LysE7), is located downstream of the colicin E7 structural gene in the ColE7 operon. Lysis protein was essential for colicin release and causes a decline in culture turbidity as well as lethality of the host cell when overproduced. Western blotting analysis of the level of LysE7 in the wild-type and csrA mutant strains was examined.

在本论文中，我们进一步发现到CsrA对於cel 基因的表现具有抑制的现象。cel 基因位在质体ColE7-K317上的E7大肠杆菌操纵子，可以制造出溶菌蛋白质(lysis protein， LysE7)，协助大肠杆菌素及免疫蛋白质的复合体运送至菌体外；但当菌体产生过多的溶菌蛋白质，则会导致宿主细胞的死亡，所以溶菌蛋白质的表现必须受到严密的调控，避免菌体产生过量，而CsrA则是目前第一个被发现到能调控溶菌蛋白质表现的因子。

I'm only slightly ashamed to admit that I found myself hoping that the strike would shut the Academy Awards down; that for once, in a year of such cinematic bounty and variety, appreciation for the best movies could be liberated from the pomp and tedium of Hollywood spectacle. It's not that I'm against the spectacle as such. I'm hardly immune to the tears, and political speeches, or low-cut dresses. I even hum along with the overproduced, underwhelming renditions of the nominated songs. And I genuinely love the film industry's earnest tributes to its past: the lifetime achievement awards, the sentimental statuettes bestowed on venerated old-timers, the clips and stills of the recently departed.

我对那些眼泪、政治演说、或者低胸礼服几乎毫无抵抗力；我甚至会在歌手们演唱那些生产过剩、陈词滥调的提名歌曲之时跟着哼唱；而且我也全心全意地喜爱电影行业对自己过去的时光最为诚挚的献礼：终身成就奖、发放给崇敬老辈人们的伤感小雕像、还有那些刚刚逝去之人的画面和照片。

According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm～150rpm时取得最大值，此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世，形体几无变化。