查询词典 ovalbumin

Coli BL21 and purified. The binding activities of immune sera to three EDs were examined with ELISA and Western blotting analysis.②Asthma model was induced with ovalbumin in mice of vaccine group, control vector group and asthma group. Airway pressure-time index and number of cells in BALF were checked. The quantity of goblet cells in bronchiole and the level of MUC5AC mRNA in lung were investigated by PAS staining and RT-PCR assay.

采用ELISA和Western blotting法检测免疫血清抗体对ED的亲合力。2、对DNA疫苗组小鼠制备OVA致敏哮喘模型，检测气道压力峰值—时间指数、支气管／肺泡灌洗液中炎性细胞数量，PAS特染观察小支气管杯状细胞数量及RT-PCR法检测肺组织中粘蛋白MUC5AC mRNA水平，免疫组化和RT-PCR法检测GM-CSF的蛋白与mRNA水平。

Results The extract remarkably inhibited the contraction caused by histamine and acetylcholine, as well as the ovalbumin sensitized allergic constriction of isolated ileum smooth muscle of guinea pigs. It could inhibit the increase of capillary permeability induced by acetic acid in mice, inhibit the mice paw edema produced by carrageenin and reduce the production of PGE 2 in inflammatory tissues.

结果：辛夷二氯甲烷提取物对组胺、乙酰胆碱（Ach引起的豚鼠离体回肠收缩具有显著的抑制作用，并能抑制卵白蛋白所致的豚鼠离体回肠过敏性收缩，对HAc引起的小鼠腹腔毛细血管通透性增高具有抑制作用，能显著抑制角叉菜胶引起的小鼠足肿胀，并减少前列腺素E2（PGE2）在炎症组织中产生。

And each group had 10 rats. After sensitization by injecting ovalbumin and inducing asthma successfully, different preventive and curial treatments were given to them for 6 days.

卵白蛋白致敏并诱喘成功后，各治疗组分别予内服桂龙咳喘宁、地塞米松和穴位贴敷"咳喘宁"贴膏进行治疗。

Methods: The asthmatic guinea pig model was established with the ovalbumin challenge methods. Forty-eight guinea pigs were randomly divided into six groups: normal control group,asthma model group,DXM –treated group,three dose groups of Jianerqiangshen electuary.

采用卵蛋白致敏法制备豚鼠哮喘模型，将豚鼠随机分为6组，即空白对照组、模型组、阳性对照组、中药3个剂量组，分别观察各组引喘潜伏期

Material and MethodsRA Rabbit Model group: 15 early RA rabbits of the same weight and variety Control group:10 normal rabbits of the same weight and varietyMethods of making RA rabbit model:elect 15 normal rabbits of the same weight and variety, dissolve Ovalbumin in 0.9% sodium chloride solution, to make the solution of 20mg/ml concengtration, blend the same quantity of complete Freund′s Adjuvant equably ,inject the mixed solution into endermic tissue of the rabbit′s scapular section, making the rabbits allergic, inject 1 ml of the mixed solution to one rabbit every time, inject 1 ml of the mixed solution in 5 different places of the rabbit′s scapular section, inject the rabbit one time every week,inject 3 weeks continuously,it turned out to be that the rabbits will be allergic, inject Ovalbumin blent with the 0.9% sodium chloride solution into the knee joint cavity of the rabbits in the fourth week, 5 mg Ovalbumin every knee joint cavity,two knees of every rabbit of the 15 rabbits are both injected,the arthrosis diameter and the exterior temperature of the knees will be added obviously in 24 hours,and they will drop gradually,at the time of the 14th or 21th day, the arthrosis diameter and the exterior temperature of the knees will get to the balance time,the incidence rate of RA is 100%.after the RA model succeeds,it is the early time of RA from the first week to the fourth week,after the fourth week,it is the late time of RA, the cartilage of the femoral condyle and the subchondral bone cortices will be changed unrecoverily.

资料与方法RA模型组：早期RA家兔模型15只，品种及体重接近。对照组：正常家兔10只，品种及体重接近。RA家兔模型制作方法：选取15只成年家兔，体重、品种接近，将卵蛋白溶解于生理盐水，配成浓度为20mg/ml的溶液，与等量完全弗氏佐剂混匀，注入家兔肩胛区皮下致敏，每只家兔每次注射1ml，于肩胛区5个不同区域注射，每周一次，连续注射3周而致敏，第4周向膝关节腔注射卵蛋白生理盐水溶液，每只关节腔注射5mg卵蛋白，15只家兔膝关节全部注射，24h内此关节直径和表面温度大幅度上升，以后缓慢下降，至14～21d达到平台期，发病率达100%。造模成功后第1～4周为早期改变，第4周以后出现不可逆的关节软骨及骨破坏。采用高频超声对RA模型组与对照组的膝关节髌上囊液体厚度、滑膜及股骨髁软骨厚度及软骨下骨皮质的回声情况进行对比观察。结果RA组模型组膝关节髌上囊积液及滑膜的厚度明显厚于对照组，其股骨髁软骨的厚度与对照组相比无明显差异，其软骨下骨皮质与对照组相比无明显改变。

METHODS:Guinea-pigs were sensitized by ovalbumin and the effects of LMWH on the constriction of ileum smooth muscle induced by OA were observed in vitro;The effects of LMWH on the degranulation of pericranium mast cell induced by OA were observed throu gh the passive anaphylaxis ratmodel which was made by vaccinating antiserum at pericranium;Licking response was induced by 4-aminopyridine(4-AP) scat scruff in mice,then the effect of LMWH on licking response was studied the capillary p emeabilty increasing induced by glactal acetic acid and the effect of LMWH on ca pillary pemeability were studied.

用卵白蛋白(ovalbumin，OA)免疫豚鼠造成过敏模型，观察LMWH对OA所致的豚鼠离体回肠平滑肌收缩的影响；在大鼠颅骨骨膜接种抗血清使肥大细胞被动致敏，观察LMWH对OA引起肥大细胞脱颗粒的影响；以4-氨基吡啶诱发小鼠舔体反应，观察LMWH对舔体反应的影响；以冰醋酸使小鼠毛细血管通透性增加，观察LMWH对通透性的影响。

The existence of dextran sulphate to the native ovalbumin leads to decrease about 60％ in fluorescence intensity following high pressure treatment. Complexation of polysaccharide with ovalbumin seems to protect the protein against pressure induced conformational change and aggregation; The presence of τ-carrageenan in glycinin solution is also found to protect the protein against pressure.

经超高压处理后，加入硫酸右旋糖苷的鸡蛋清蛋白溶液中的ANS荧光强度显著降低，降低约60％；这表明，硫酸右旋糖苷的加入可以和鸡蛋清蛋白相互作用，从而在超高压处理中起到稳定蛋白质天然构象的作用；对于大豆豆球蛋白溶液而言，ι-角叉菜胶的加入有维持高压下大豆豆球蛋白构象稳定性的作用。

Circular dichroism spectroscopy measurements also reveal that the ordered structure content of α-helical and β-structure of ovalbumin decrease, and the unfolded random structure is enhanced with increasing pressure; While the existence of dextran sulphate to the native ovalbumin, there are no apparent change on the secondary structure of ovalbumin; It seems same to glycinin.

圆二色性光谱分析表明，经超高压处理后，鸡蛋清蛋白分子中规则的二级结构减少，而部分规则的二级结构和无规卷曲含量增加。加入硫酸右旋糖苷的鸡蛋清蛋白溶液经超高压处理后其二级结构中各部分的含量变化不大：对于大豆豆球蛋白，加入硫酸右旋糖苷后，大豆豆球蛋白分子经超高压处理后其有序结构的减少及无规卷曲的增加都相对减缓。

In order to develop a fast ELISA detection kit, clenbuterol was diazotized firstly and then coupled to bovine serum albumin and ovalbumin to synthesis immunogen and coating antigen respectively.

首先，重氮化的 CL 分别与牛血清白蛋白(bovine serum albumin， BSA)和卵清白蛋白(ovalbumin， OVA)交联，制备成免疫抗原 CL-BSA 和包被抗原 CL-OVA。

The rats inasthma model and dexamethasone group were actively sensitized bysubcutaneous injection of ovalbumin and suscitated by an aerosolof 1% OVA 2 weeks after sensitization.

分别给予哮喘模型组和地塞米松给药组大鼠皮下注射致敏物质卵清蛋白(ovalbumin，OVA)，致敏二周后，用含1％的OVA悬浮微粒激发。

Do you know, i need you to come back

你知道吗，我需要你回来

Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书，王祥生，李德昌。安徽省首次发现嗜群血蜱。