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Use constituent piece to stick mural way depart to foster Niu Er to become fiber cell above all, after classics generation is rarefied, fat simple way will be contained double the skin of the 4th acting ox that outside conveying carrier to turn into body, the particularity of person bacteriolysis enzymatic mammary gland that chooses number fosters becomes fiber cell, g/mLG418 chooses 500 μ 2 weeks, g418 halve continues to develop 3 generation of 2 ～, catch the cell after choosing to undertake PCR detects to turning next reach nucleus analysis.

首先采用组织块贴壁法分离培养牛耳成纤维细胞，经传代纯化后，脂质法将含有双重筛选标记的人溶菌酶乳腺特异性表达载体转入体外培养的第4代牛皮肤成纤维细胞，500μg/mLG418筛选2周，G418减半继续培养2～3代，然后对转染筛选后的细胞进行PCR检测及核型分析。

Although move free industry to get global banking crisis,affect not quite, but, move free company to do to the foreign country " marry the garment ", it is to live under another's roof forever, how should the enterprise realize self-improvement truly free-standing, the practice of company of summit of a person of extraordinary powers is job of the bag outside using exercises a procession, hold to the journey that starts formerly.

虽然动漫行业受全球金融危机影响不大，但是，给外国动漫公司做"嫁衣"，永远是寄人篱下，企业该如何真正实现自强自立，豪峰公司的做法是用外包工作锻炼队伍，坚持走原创之路。

And pass online outside the means of the bag, job of invite public bidding can be used after finding right person through afore-mentioned means (Http://zhaobiao.taskcn.com) or trade quickly means instalment, after the job begins, employer puts task fund be in charge of task treasure (Http://pay.taskcn.com), achieve business demand till working achievement, ability loan gives worker people.

而通过在线外包的方式，通过上述的方式找到合适的人后可以使用招标任务(http：//zhaobiao.taskcn.com)或快速交易方式分期付款，当工作开始后，雇主就把任务资金存管到任务宝(http：//pay.taskcn.com)，直到工作成果达到企业需求，才放款给工作者们。

A person to the outside areas travel, he brought a big pile of baggage to want a rental car.

一个人到外地旅行，他带着一大堆行李要了一辆出租车。

The Great Highland Bagpipe5 is the most common bagpipe in Scotland. It is a very loud instrument and is therefore often played outside.

风笛多种多样，其中高地大风笛是苏格兰最为常见的风笛，它的声音高亢嘹亮，也因此常在户外演奏。

Their precursors were such British moderates as PT Forsyth, RW Dale, and James Denney; their colleagues outside the Church of England, theologians such as HR Mackintosh; and their successors (though the term was seldom applied), such luminaries as TW Manson, JS Whale, Donald and John Baillie, and perhaps even CS Lewis.

他们的前体，如英国温和派作为铂佛塞斯的组建戴，詹姆斯，丹尼；同事以外的英国教会，神学家，如心率麦金托希；及其继承人（虽然任期内，很少有人应用），这种灯具作为星期日曼森， js鲸鱼，唐纳德和约翰baillie ，甚至路易斯。

One of the most impressive features is that 100% of outside cabins will have balconies.

最令人印象深刻的特点之一是车厢内外的100％将有阳台。

At the same time, Moscow residents were warned to avoid staying too long outside, and shut doors and windows tightly, for balefire indulged wilfully and the capital was folded in thick smoke

在此同时，莫斯科居民因附近野火肆虐，首都被浓浓灰烟笼罩，昨天各家各户都接获警告，要他们尽量避免在外长时间停留、并紧闭门窗。

Kaleri didn't believe the sound was from balky equipment; rather it seemed to originate from outside.

他认为那声音应该不是太空站机械运转不顺所引起，比较像是由太空站外部传来的。

Kaleri did not believe the sound was from balky equipment; rather it seemed to originate from outside.

他认为那声音应该不是太空站机械运转不顺所引起，比较像是由太空站外部传来的。

As of Tuesday, Google's results were still censored in China.

截至周二，谷歌的搜索结果仍受中国审查。

In order to make the positive action increase and negative one decrease, the sub-forces of the social factors must be adjusted to form a centripetal force.

在这一过程中，人的主体性发挥是社会有机体健康发展的灵魂。