查询词典 ouabain

Tyrosine binding occurred also under the condition of 4℃ for 10min or when the transportation of tyrosine into the cells was inhibited by ouabain.

用哇巴因抑制酪氨酸向细胞内的转运后，〓-酪氨酸的特异结合依然存在，放射菌酮也不能影响〓-酪氨酸结合。

In short, we first found EO played an important role in hypertension in 1k1c hypertensive rats. On this basis, we put forward a plan to prepare a new depressor to direct against EO. Then we proved that ouabain polyclonal antibody can decrease significantly blood pressure in 1k1c rats.

总之，本课题首先在1k1c高血压大鼠中证实EO在高血压的发生中起着较重要的作用，在此基础上我们提出了研制一种针对于EO的降压新药的设想，并在1k1c高血压大鼠中证明哇巴因多克隆抗体具有明显的降压作用。

The effect of some oxygen free radical scavengers, Superoxide Dismutase and Sodium Selenate, on Ouabain induced toxicity of isolated heart were studied by the Langendorff apparatus.

用Langendorff离体心脏灌流制备，研究氧自由基清除剂：超氧化物歧化酶和亚硒酸钠对哇巴因所致心脏毒性的影响。

It is concluded the result suggest that in the 569-764 nucleotides region of Ouabain combined sites of sodium pump α1 gene in Kazakans of Xinjiang might not exist gene variant or the frequency of variant might be extraordinary low.

钠泵 。1基因变异所致的钠离子转运障碍可能起着联系盐敏感与高血压的桥梁作用址'3二。目前针对钠泵基因与人类高血压关系的研究甚少。为探讨人类高血压的形成过程中

Each medicine used in the study totally has obivious effect on lens sodium-pump. Either on the molecular and cellular level or on the histological and organic level , they all have their distinctive demonstration. 1.Ouabain、Digoxine、DMSO decrease the expression of αsubunit of sodium pump on mRNA level , the cell morphology and ultramicroscopic structure demonstrated destructive effect,it indicates the cause of cataract of the medicines.2.D-thyroxine、Amphotericin B、Vitamin E increase the expression of αsubunit of sodium pump on mRNA level ,from the demonstration of the cell morphology and ultramicroscopic structure ,it indicates the medicines have protective or anti-distructive effect on lens.3.The medicines have isoform-specific action on lens sodium-pump,it indicates exploitting isoform-specific medicine has a promising clinical application.4. Lens sodium-pumps are inhibited probably lead to cataract, so in clinical medication, especially to the cardiovascular doctor, while he is using the sodium-pump inhibitors, he should pay more attention to adjust the dosage and duration of the drug therapy. 5. To creat the animal model of cataract through inhibiting lens sodium-pump, whether it can be a kind of classical method or not, it is worthy of further research.

本研究所选用的药物对晶状体钠泵均有明显的影响作用，在分子水平、细胞水平、组织器官水平均有特异的表现：1、哇巴因、地高辛、二甲基亚砜三种药物可降低晶状体钠泵α亚单位在 mRNA水平的表达，在细胞形态和超微结构上表现为损伤效应，提示了其潜在的导致白内障的可能。2、D甲状腺素、两性霉素B、维生素 E三种药物可升高晶状体钠泵α亚单位在 mRNA水平的表达，从细胞形态和超微结构上的表现，提示了其对晶状体有保护作用或抗损伤效应。3、药物作用于晶状体钠泵具有重整异构特异性，提示了开发出特异的具有重整异构作用的药物是极有临床应用前景的。4晶状体钠泵抑制后可能导致白内障的发生，故临床用药，尤其是心内科医生在使用这些钠泵抑制剂时应注意调整用药剂量及药物疗程。5 通过抑制晶状体钠泵来制作白内障动物模型能否成为一种经典的制作方法，还有待于进一步研究。

RESULT: 1.Ouabain act on lens sodium-pump,under the LM,lens anterior capsular membrane discontinuous,epithelium cells clustered,occluding zonule seperated,lens fiber layers fractured.Under the EM,cells totally hollowed,mitochondria swelling,myelin figure appeared.RT-PCR examine the expression condition of αsubunit of sodium pump on mRNA level,α1、α2 and α3-isoform are all decreased.2.Digoxin act on lens sodium-pump,under the LM,lens cell oedema,linkage distructed,extensive exfoliation.Under the EM,plasma appeared little half-transparant hollow region,mitochondria swelling and ridge disappeared. RT-PCR examine,α1、α2 and α3-isoform are all decreased.3.Amphotericin B act on lens sodium-pump,under the LM,lens epithelium cells linked tightly,arranged in-line,lens fiber layers arranged tightly and regularily.Under the EM,abbundant cellular organes,exuberant cells function indicated. RT-PCR examine the expression condition of αsubunit of sodium pump on mRNA level,α1 and α3-isoform are increased significantly,demonstrated isoform-specific action.4D-thyroxine act on lens sodium-pump,under the LM,lens plasmalemma integrated,cells arranged tightly and regularily.Under the EM,nucleus fission appeared,desmosome half-desmosome and tensile microfilaments linked the cells. RT-PCR examine,α2 and α3-isoform are increased, also demonstrated isoform-specific action.5.Vitamin E act on lens sodium-pump,under the LM,lens anterior capsular membrane continuous and smooth,epithelium cells tightly linked,lens fiber layers appearede hollow region occasionally.Under the EM,lateral membrane high density belt appeared,abundant nucleolus. RT-PCR examine,onlyα1-isoform are increased, demonstrated significantly isoform-specific action.6.DMSO act on lens sodium-pump,under the LM,lens anterior capsular membrane slightly thicker,cells linkage partly distructed.Under the EM,plasmalemma denaturation,mitochondria swelling.RT-PCR examine,α1、α2 and α3-isoform are all altered slightly and haven't significant meanning.

结果：1、哇巴因作用于晶状体钠泵后，光镜下晶状体前囊膜断裂、上皮细胞聚积、闭合连接分离、纤维板层裂隙，电镜下全层细胞空泡化、线粒体肿胀出现髓样结构，RT-PCR法检测晶状体钠泵α亚单位α1、α2及α3三种重整异构体在mRNA水平的表达均减弱。2、地高辛作用于晶状体钠泵后，光镜下晶状体细胞水肿、细胞连接破坏、广泛剥离，电镜下胞质见少许半透明空化区、线粒体肿胀嵴消失，RT-PCR法检测晶状体钠泵α亚单位α1、α2及α3三种重整异构体在mRNA水平的表达均减弱。3、两性霉素B作用于晶状体钠泵后，光镜下晶状体上皮细胞紧密连接、线状排列、纤维板层紧密规整，电镜下细胞器丰富、细胞功能旺盛，RT-PCR法检测α1及α3表达显著增强、具有一定的重整异构作用特异性。4、D甲状腺素作用于晶状体钠泵后，光镜下晶状体质膜完整、细胞排列紧密规整，电镜下胞核见分裂像、细胞间有桥粒、半桥粒及张力微丝，RT-PCR法检测α2及α3表达均增强、亦有一定的重整异构作用特异性。5、维生素E作用于晶状体钠泵后，光镜下晶状体前囊膜连续光滑、上皮细胞紧密连接、纤维板层偶见空化，电镜下囊侧膜内有高电子密度带、细胞核仁丰富，RT-PCR法检测仅有α1的表达显著增强、具有极强的重整异构作用特异性。6、二甲基亚砜作用于晶状体钠泵后，光镜下晶状体前囊膜轻度增厚、细胞连接部分破坏，电镜下质膜变性、线粒体肿胀，RT-PCR法检测晶状体钠泵α亚单位α1、α2及α3三种重整异构体在mRNA水平的表达无显著改变。

Because ouabain polyclonal antibody made blood pressure reduced for shorter time and possessed slightly allergy, we aimed to prepare a new depressor, ScFv, with high specificness by advanced phage display technique. The heavy-chain and kappa light-chain variable region gene repertoir of immunoglobin were amplified individually with heavy chain and light chain primers of Pharmacia Corporation and assembled into ScFv by a linker with overlap extension PCR.

针对哇巴因多克隆抗体存在的降压时间持续较短及存在轻微的过敏反应等缺点，本研究利用先进的噬菌体抗体库技术，拟研制开发一种具有高度选择性的降压新药--哇巴因单链抗体。

RESULTS Ouabain-like immunoreactivities were observed abundantly in the inner zone of adrenal cortex, known as the zone reticularis of SD rats and Macaca mulattas; weakly in the posterior pituitary of SD rats, but nothing in the hypothalamus.

结果 在大鼠和恒河猴的肾上腺皮质内侧网状带细胞中可以观察到丰富的哇巴因免疫反应阳性物质，垂体后叶有较弱的哇巴因免疫反应阳性物质，而下丘脑并未发现有哇巴因免疫反应物质存在。

Ouabain-LI chiefly exist in the zone reticularis of adrenal cortex of SD rat and Macaca mulattas. The morphological evidence shows that EO is a novel adrenocortical steroid hormone.

哇巴因免疫反应阳性物质主要存在于SD大鼠和恒河猴的肾上腺皮质网状带，为EO作为一种新的肾上腺皮质激素提供了形态学依据。

However, as the name（read－only memory）implies, CD disks cannot be written onorchanged in any way.

然而，正如其名字所指出的那样，CD盘不能写，也不能用任何方式改变其内容。

Galvanizes steel pallet is mainly export which suits standard packing of European Union, the North America. galvanizes steel pallet is suitable to heavy rack. Pallet surface can design plate type, corrugated and the gap form, satisfies the different requirements.

镀锌钢托盘多用于出口，替代木托盘，免薰蒸，符合欧盟、北美各国对出口货物包装材料的法令要求；喷涂钢托盘适用于重载上货架之用，托盘表面根据需要制作成平板状、波纹状及间隔形式，满足不同的使用要求。