查询词典 ouabain

Aim To observe the effects of ouabain and aconitine on APD and ion channels in isolated guinea pig and rat ventricular myocytes; to elucidate the action mechanisms of these two drugs and set up new arrhythmic models on cellular level.

目的 观察哇巴因和乌头碱对豚鼠和大鼠心肌单细胞离子通道的作用，确定两药诱发心律失常时离子靶点和最佳靶点，建立细胞水平的心律失常模型。

A large quantity of evidence has showed that higher EO might be one of the pathogenic factors of hypertension. EO also referred to endogenous sodium pump inhibitor, endogenous digitalis-like substance or endogenous digoxin-like factor before, is a recently identified sterone hormone that might be secreted from adrenal cortex.

内源性哇巴因(endogenous ouabain， EO)是新近发现主要由肾上腺皮质所分泌的一种类固醇激素，大量证据表明，体内EO含量升高可能是高血压的发病因素之一。

Methods Animals were randomly and equally divided into five groups: saline control group, positive drug control group, low-dose, medium-dose and high-dose crocetin group. Animals were anesthetized with urethane (1.2 g/kg), then followed by the perfusion of aconitine (10 μg/mL) through caudal vein in rats, or of ouabain (9 μg/mL) through external jugular vain in guinea pigs in a constant velocity of 0.1 mL/min, and the emergence time of ventricular fibrillation, ventricular extrasystole, ventricular tachycanlia and cardiac arrest were then detected and recorded. The recorded time was then translated to the cumulated amount of aconitine and ouabain. Experimental arrhythmia was induced by rapid injection of 4% CaCl2 (140 mg/kg), and the emergence time, persistence time, incidence and death of arrhythmia after the administration were observed.

将实验动物随机分为生理盐水组，阳性药物对照组，藏红花酸高、中、低剂量组，SD大鼠采用1.2 g/kg乌拉坦麻醉后，从尾静脉匀速(0.1 ml/min)灌注10μg/ml乌头碱溶液，豚鼠麻醉后从颈静脉匀速(0.1 ml/min)灌注9μg/ml哇巴因溶液，观察出现室性期前收缩、室性心动过速、室颤和心脏停搏的时间，然后换算成乌头碱和哇巴因的累积量；快速推注4%氯化钙(140 mg/kg)诱导大鼠心律失常，观察给药后心律失常出现时间、持续时间、心律失常发生数及死亡数。

Objective:To investigate the role of endogenous ouabain in the pathogenesis of hypertension.

目的：探讨内源性哇巴因在高血压发病中的作用。

Until now, it was the first time that the ouabain ScFv was cloned and sequenced by us in the world.

至此，我们在国际上首次克隆并测序了哇巴因单链抗体基因。

This study was aimed to investigate the effects of ouabain at low concentrations on growth regulation in various leukemia cell lines and to determine the therapeutic potential of ouabain in leukemia.

本研究目的在于通过研究低浓度哇巴因对不同白血病细胞株生长的影响，探讨哇巴因用于白血病临床治疗的可行性。

The enzyme-linked immunosorbent assay that we have established for endogenous ouabain is simple, precise, specific and lower cost, which can be used in the clinical and experimental study on endogenous ouabain.

该方法具有方便、快速、准确、特异和成本低的优点，可用于对内源性哇巴因的临床与实验研究。

According to the fact that EO has played an important role in the development of hypertension, we put forward a hypothesis that ouabain antibody might reduce blood pressure. It was confirmed the blood pressure was dropped down for 3 hours after 1k1c hypertensive rats was injected with ouabain polyclonal antibody for 3 hours.

EO在高血压的发生中起着较为重要的作用，由此我们提出了哇巴因抗体可能具有降压作用的假说，并在对1k1c高血压大鼠静脉注射哇巴因多克隆抗体后3小时的血压连续观察中证实了该假说。

Methods ATP enzyme kit method, respectively, set 10,50,250,500,800 μM / L of TMT and Ouabain experimental tubes, pipes and control settings TMT, Ouabain corresponding dose of control tubes, each of the three parallel A total of 66, were measured by enzyme activity NKATP the tube.

参照ATP酶试剂盒测定方法，分别设定10、50、250、500、800μM/L的TMT和Ouabain实验管，设定空白对照管和TMT、Ouabain相应剂量的对照管，每组平行三份共66管，分别测定各管NKATP酶活力。

Get the neonate Sprague-Dawley rats medulla spinalis, through the conventional primary cell culture procedure, we use the method of difference adherence time and get the motor neurons of anterior spinal cord. When the neuron is in maturity, we make the mechanical injury model in vitro. All the models were divided into four groups: group A is control group; group B is 100μM ATP group; group C is 100μM ATP+20μg/ml suramin group and group D is 100μM ATP+10μM ouabain+10μg/ml Thapsigargin group. Culture the four groups neurons, after one day, we count the motor neuron, observe the survival and activity of neurons through MTT shade selection experiment, use flow cytometry to analyze the percentage of apoptosis of motor neurons of anterior spinal cord and detect the expression of protein p-GSK-3β(ser9) through Western-Blot technology.

方法取新生大鼠脊髓，通过常规的原代细胞培养程序，采用差速贴壁法分离出大鼠脊髓前角运动神经元，培养成熟后制作神经元机械损伤体外模型，分为A组：对照组、B组：100μMATP组、C组：100gMATP+20μg/ml Suramin组和D组：100μM ATP+IOμM Ouabain+10μg/ml Thapsigargin组，对各组机械损伤的运动神经元进行培养，1天后分别进行运动神经元计数、MTT比色实验观察运动神经元的存活及活性、流式细胞仪分析机械损伤的脊髓前角运动神经元凋亡百分率和Werstern Blot技术检测p-GSK-3β(ser9)的表达。

However, as the name（read－only memory）implies, CD disks cannot be written onorchanged in any way.

然而，正如其名字所指出的那样，CD盘不能写，也不能用任何方式改变其内容。

Galvanizes steel pallet is mainly export which suits standard packing of European Union, the North America. galvanizes steel pallet is suitable to heavy rack. Pallet surface can design plate type, corrugated and the gap form, satisfies the different requirements.

镀锌钢托盘多用于出口，替代木托盘，免薰蒸，符合欧盟、北美各国对出口货物包装材料的法令要求；喷涂钢托盘适用于重载上货架之用，托盘表面根据需要制作成平板状、波纹状及间隔形式，满足不同的使用要求。