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They conta i ned full genes ORFs and proved to have been prolongated effectively. The result showed that the seqences of 14 genes were genes known previously and 6 genes were new .We separately chose one seqence from known and the other from unknown genes to make full-length cDNA annotation. Their code number was EST contig110 and EST contig133. We designed specific primers to amplify the two genes by RT-PCR, and then sequenced. The result was obtained as expected and proved that silico cloning genes was reliable.

对20条contigs进行了基因注释，证实它们均得到了有效延伸，具有完整开放阅读框，其中已知的猪基因序列14条，猪新基因序列6条，分别从已知猪基因序列和未知猪基因序列中各抽取一条感兴趣序列进行全长cDNA的预测，它们的编号分别为EST contig110和EST contig133，设计特异引物对两条候选序列进行克隆与测序，结果与电子克隆所得序列一致，证实了电子克隆的有效性。

It ought to be solemnized with pomp and parade, with shows, games, sports, guns, bells, bonfires, and illuminations, from one end of this continent to the other, from this time forward forever more.

它应该与盛大游行和婚礼，以表演，游戏，体育，枪支，钟，篝火，以及灯饰，从一本大陆一端到另一端，从这个时候提出永存。

It ought to be solemnized with pomp and parade.from one end of this continent to the other,from this time forward forevermoreJohn Adams.

&它[1776年7月2日]应作为诞生之日来纪念……应用壮观的典礼和游行来隆重庆祝……从这片大陆的这头到那头，从此时到永远&约翰·亚当斯

"It [July2,1776] ought to be commemorated as the day of deliverance... It ought to be solemnized with pomp and parade... from one end of this continent to the other, from this time forward forevermore".

&它［1776年7月2日］应作为诞生之日来纪念……应用壮观的典礼和游行来隆重庆祝……从这片大陆的这头到那头，从此时到永远&

It ought to be solemnized with pomp and parade.from one end of this continent to the other,from this time forward forevermoreJohn Adams.

它［1776年7月2日］应作为诞生之日来纪念……应用壮观的典礼和游行来隆重庆祝……从这片大陆的这头到那头，从此时到永远参见同义词

For this reason a prince ought to have two fears, one from within, on account of his subjects, the other from without, on account of external powers.

因此，君主必须注意两件事：其一是内部的，它来自臣民方面；其二是外部的，它来自外国势力。

The 1920 saw the emergence of a new psychological school called Gestalt psychology.it was founded by a group of German psychologists such ad Max Wertheimet,Kurt Koffka,and Kurt Lewin.most of their early research was focused on the area of perception,aiming at the exploration of the relationship between parts and whole in people's petceptual experience.from their experiment,they found that people perceived objects and scenes as organized wholes beforethey noticed their component parts.For example,when people catch sight of a desk,they will recognize the desk asa whole before noticing its legs and other parts.that is why they use the word Gestalt,which means roughly"organized shape"or "whoe form" in English,to name their school of psychology

在1920年看到了一个新的心理称为塔psychology.it学校的出现，是由德国心理学家等广告最大Wertheimet，科特考夫卡和库尔特其早期研究Lewin.most成立于知觉的领域集中，瞄准之间的部分和整个人民的关系探索单曲petceptual 经验。from他们的实验中，他们发现，人们认为的对象和有组织的整体，场面beforethey发现自己的组件部分。F例如，当人们捕捉，桌上看到，他们将承认前注意到它的腿和其他parts.that桌子阿萨整体他们为什么用这个词塔，这意味着约&组织型&或&所有形式&的英文，说出他们的心理学校。

Wild boar that have lost legs to landmines can be seen hobbling out from the forests, and when two gorals face each other from either side of the border fence it is hard to tell which one is in the safest area.

我们可以看见踩上地雷、失去腿的野猪在森林中跛行；当两只斑羚在边界的篱笆两侧相对时，很难分清哪边是安全区域。

When the Dalmatian is standing, the hind legs, viewed from the rear, are parallel to each other from the point of the hock to the heel of the pad.

飞节：位置低，当大麦町犬站立时，从后面观察，后腿从飞节开始到足爪这部分彼此平行。

In other words, this is yet another low-brow laff-a-thon from the Broken Lizard gang that's likely to draw a bigger audience on DVD than it did in theaters, especially since there's a lot of duds to sit through while waiting for the next big beer-belly-laugh.

换言之，这又是低额头laff - 1和平面吞from断蜥团伙，这可能会更多的观众绘制的DVD运动不仅仅它在电影院，特别是因为很多是一个哑弹参加通过而下一个大的啤酒肚皮笑等候。

With Death guitarist Schuldiner adopting vocal duties, the band made a major impact on the scene.

随着死亡的吉他手Schuldiner接受主唱的职务，乐队在现实中树立了重要的影响。

But he could still end up breakfasting on Swiss-government issue muesli because all six are accused of nicking around 45 million pounds they should have paid to FIFA.

不过他最后仍有可能沦为瑞士政府&议事餐桌&上的一道早餐，因为这所有六个人都被指控把本应支付给国际足联的大约4500万英镑骗了个精光。