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Methods A nested RT-PCR was used for detecting one fragment of SARS-CoV RNA in oropharyngeal swabs from 3 SARS probable patients ,4 SARS suspect patients and other 27 patients with fever in Hangzhou, and the nested RT-PCR product from one SARS probable patient was sequenced.Meanwhile in these 3 SARS probable patients, other three RT-PCR methods, including a hemi -nested RT-PCR targeted for another fragment of SARS-CoV RNA, a real-time RT -PCR and a modified nested real-time RT-PCR, were employed to detect SARS-Co V RNA.Results Two positives were found in the 3 SARS pro bable patients, and none positive in 4 SARS suspect patients and other 27 patien ts with fever, using the nested RT-PCR.

方法对2003年杭州市3例严重急性呼吸综合征临床确诊患者、4例疑似和27名医学观察者的咽拭子标本用巢式实时RT-PCR检测SARS-CoV核酸，对阳性扩增产物进行核酸序列测定，同时对3例患者的标本应用半巢式RT-PCR检测另一位点SARS-CoV核酸片段，并应用常规实时RT-PCR技术及改良实时RT-PCR技术进行检测。

In view of geological records preserved from different regions of the world are not identical, it's suggested that, when correlating the Ordovician-Silurian boundary, at the global boundary stratotype section, the FAD of N. persculptus be selected as the biomark, the transgression event identical to the FAD of N. persculptus as the auxiliary physical mark and the mass extinction and chemical anomaly events respectively as the auxiliary biomark and chemomark. In some regions with few graptolite, the Ordovician-Silurian boundary should be indicated by the auxiliary marks, such as the transgression event, mass extinction or chemical anomaly event, with the other fossil such as conodont nearest to other geological events as the provincial biomark for correlation. In other regions with much stratigraphic hiatus originated from the regression, the Ordovician-Silurian boundary should be marked by the regression-transgression event nearly relevant to the N. persculptus Zone.

鉴于不同地区留下的地质记录不完全相同，奥陶/志留系界线对比方法不同：在界线层型剖面上，以N.persculptus的首现为生物标志，以与之基本一致的海侵事件为辅助的物理标志，以生物集群绝灭事件和化学异常事件为辅助的生物和化学标志；在笔石极不发育的地区，则以海侵事件、生物绝灭事件或化学异常事件为辅助标志，以与其相距最近、并且具有区域对比性的其它门类化石作为界线对比的生物标志；对于由于海退事件所造成地层缺失很多的地区，则以海退/海侵事件作为划分界线的物理标志，并以相映的海侵事件的开始作为奥陶/志留系的分界。

In viewof geological records preserved from different regions of the world are not identical, its suggested that, whencorrelating the Ordovician-Silurian boundary, at the global boundary stratotype section, the FAD of N.persculptus be selected as the biomark, the transgression event identical to the FAD of N. persculptus as theauxiliary physical mark and the mass extinction and chemical anomaly events respectively as the auxiliarybiomark and chemomark. In some regions with few graptolite, the Ordovician-Silurian boundary should beindicated by the auxiliary marks, such as the transgression event, mass extinction or chemical anomaly event,with the other fossil such as conodont nearest to other geological events as the provincial biomark forcorrelation. In other regions with much stratigraphic hiatus originated from the regression, theOrdovician-Silurian boundary should he marked by the regression-transgression event nearly relevant to the N.persculptus Zone.

鉴于不同地区留下的地质记录不完全相同，奥陶／志留系界线对比方法不同：在界线层型剖面上，以N.persculptus的首现为生物标志，以与之基本一致的海侵事件为辅助的物理标志，以生物集群绝灭事件和化学异常事件为辅助的生物和化学标志；在笔石极不发育的地区，则以海侵事件、生物绝灭事件或化学异常事件为辅助标志，以与其相距最近、并且具有区域对比性的其它门类化石作为界线对比的生物标志；对于由于海退事件所造成地层缺失很多的地区，则以海退／海侵事件作为划分界线的物理标志，并以相映的海侵事件的开始作为奥陶／志留系的分界。

The main conclusions from the works presented in this thesis can be as this: for the Galactic OH 1612MHz masers, the radiative pumping mechanism coexists with other mechanisms while some other unresolved problems need to be solved after the launch of more advanced space infrared spectrometer and the more reliable identification of the evolutionary status of the maser bearing infrared sources;the asymmetrical 53.3μm absorption line profile could become a powerful tool to assist us to observationally discriminate the radiative pumping mechanism from other pumping mechanisms in the OH masers.This is the English version of my PhD thesis.

通过本论文的研究工作，我们可以得到这样的结论：在银河系内的OH 1612MHz脉泽中，辐射抽运机制与其它抽运机制并存，而还有一些未解决的问题需要等待更先进的空间红外光谱观测设备的使用并与天体类型证认工作相配合加以解决；不对称的53.3微米吸收线轮廓特征有可能成为帮助我们从观测上对OH脉泽的辐射抽运机制加以鉴别的有力工具。

This paper has studied the physiological and biochemical changes during the period of forming strong buds of tree peony, and expect to provide academic basis for extending its view value and economic benefit . The main results are as the following:1 Under the field condition, Characteristics of photosynthesis of tree peony are very regular. From before blooming to the prophase of abducting buds that after blooming ,that is April,May and June,the net photosynthetic rate of tree peony is higher than the other period .It indicates that this period is very important for accumulating photosynthetic products of tree peony . So, if we want to get quality flower of tree peony ,it is very important to strengthen the management of tree peony cultivation and to ensure provide the fertilizer and water.2 From the middle ten days of June, with the time elapsing and with the environment conditions worsening, the characteristics of photosynthesis of tree peony decline deeply. But it is different among different tree peony breeds. For example , cv. Wu Long Peng Sheng can also has higher net photosynthetic rate, it indicates that cv. Wu Long Peng Sheng is super than the other two tree peony breeds when adapting the environment conditions and cv. Wu Long Peng Sheng has wide prospect to extend .3 The main factors of effecting the characteristics of photosynthesis are stomatal factor and non-stomatal factor ,that is the ability of photosynthesis of leaf cells. Under the environment of high light intensity in midsummer, if overshadow tree peony properly, then it can slow the declining of chlorophyll content, and increase the net photosynthetic rate and benefit for the accumulating of photosynthetic products.4 The content of soluble sugar in the root of tree peony is the highest among different organs during the period of forming strong buds . According to analyse ,we believe that the soluble sugar will compose starch then, and provide the solid base of material for the growth and development next year. It provide us strong academic basis of adopting the method of cultivating root and protecting root under thefield environment conditions .In other words ,it will help to increase the health level of the whole plant of tree peony and will be beneficial to forming quality tree peony flower if we strengthen the management of root. 5 To tree peony, the endogenesis hormone is important for its growth and development .In the prophase ,that is about the blooming of tree peony and the mid-summer ,IAA and ZR contents are higher and GA content is lower and its changes are very little, and the content of ABA is decreasing . This indicates that IAA and ZR act the important promote function for growth and development of tree peony in this period, and the function of GA is not important. Bur in the period of anniversary senescence of tree peony , the content of IAA and ZR begin to decrease ,while the ABA content has a certain rebound .T

本研究对几个牡丹品种壮芽形成过程中的某些生理生化变化做了研究，以期了解牡丹生长发育过程中生理生化的变化规律，探讨大田栽培条件下提高牡丹花质量的栽培措施和管理技术，为提高牡丹的观赏价值和经济效益提供理论依据，结果如下：1 大田栽培条件下，牡丹光合特性在生长发育过程中表现出极强的规律性，牡丹各品种的光合速率从牡丹开花前，到牡丹花谢后的花芽诱导期前期（6月10日之前），也就是在4、5、6月份，各牡丹品种的净光合速率相对与其他时间都维持在比较高的水平，这说明，在这段时间，是牡丹光合产物积累的重要时期，因此，要保证得到高质量的牡丹花，在这段时间加强对牡丹的栽培管理，保证这个时期的肥水供应十分重要。2 从6月中旬后，随时间的推移，环境条件的恶化，牡丹的光合性能剧烈下降，但是不同牡丹品种间仍有差异，比如，乌龙捧盛仍可维持较高的光合水平，这说明了乌龙捧盛这个牡丹品种对于环境的适应性较之其他牡丹品种高，有着广泛的推广前景。3 影响牡丹光合性能的主要因子是气孔因素和非气孔因素即叶肉细胞的光合能力，在盛夏高温高光强的环境条件下，对牡丹进行适当遮荫，能缓解牡丹叶绿素水平的下降，适当提高Pn，有利于光合产物的积累。4 可溶性糖在牡丹各品种的壮芽形成过程中，根中可溶性糖的含量在牡丹各部位中始终维持最高水平，分析认为这些可溶性糖在根系中进一步合成淀粉等储藏性物质，为来年牡丹的生长提供雄厚的物质基础，这为我们在大田栽培条件下对牡丹采取养根护根的栽培措施提供了有力的理论依据，即在大田栽培条件下，加强对牡丹根系的管理，有助于提高牡丹整株的健壮程度，有益于形成高质量的牡丹花。5内源激素对牡丹的生长发育调节作用是十分明显的，在前期，即开花前后和盛夏，IAA和ZR含量维持在比较高的水平，GA含量低且变化不明显，ABA含量逐渐降低，说明了IAA和ZR对牡丹在这段时间内的生长发育起到重要的促进作用，GA的作用不是很明显，ABA可能在高温高光强下有明显的升高，也说明了ABA的确有一些促进作用，但是到了牡丹周年衰老期，IAA和ZR的含量开始下降，ABA含量却有了一定程度的反弹，说明了IAA和ZR的作用开始降低，ABA起到了促进衰老的作用

Larry Taunton and I defeated our opponents from the other party after another day of campaigning and I arrived in College Park on July 19, 1963, and eager to meet the other delegates, vote on important issues, hear from cabinet members and other government officials, and visit the White House, where we hoped to see the President.

拉里。汤顿在某一天的竞选结束时击败了来自其它党派的对手。1963年7月19日，我来到了华盛顿郊外的学院公园区，渴望会见其他代表，就重要问题投票，听取内阁成员和其他政府官员讲话，参观白宫，并希望在那里见到总统。

The results of physical and chemic characteristic show that the molecular weight of R-PE is 194kD, which is differ from the other reported R-phycoerythrin's (240kD). The molecular weight of subunits were determined . The molecular weight of a and P subunits are alike for about 20kD. The molecular weight of y subunit is about 31 kD.The subunit composition of R-PE in Corallina officinalis is presumably 4 y , which is differ from 6 y that is reported by other researcher. Moreover, we must to do more other work to prove if it contains the fourth subunit.

对R-PE部分理化特性的研究结果表明，R-PE的分子量为194kD，与目前报道的多数R-藻红蛋白240kD的分子量不同；其α、β亚基分子量均为20kD左右，γ亚基分子量约为31kD，理论推测的R-PE亚基组成为_4γ，与前人报道的_6γ不同，而且其是否存在第四亚基还有待进一步证明；同时在变性条件下对R-PE的各亚基进行了分离纯化并对其各亚基可见光光谱特性作了研究。

The results of physical and chemic characteristic show that the molecular weight of R-PE is 194kD, which is differ from the other reported R-phycoerythrins (240kD). The molecular weight of subunits were determined . The molecular weight of a and P subunits are alike for about 20kD. The molecular weight of y subunit is about 31 kD.The subunit composition of R-PE in Corallina officinalis is presumably 4 y , which is differ from 6 y that is reported by other researcher. Moreover, we must to do more other work to prove if it contains the fourth subunit.

对R-PE部分理化特性的研究结果表明，R-PE的分子量为194kD，与目前报道的多数R-藻红蛋白240kD的分子量不同；其α、β亚基分子量均为20kD左右，γ亚基分子量约为31kD，理论推测的R-PE亚基组成为_4γ，与前人报道的_6γ不同，而且其是否存在第四亚基还有待进一步证明；同时在变性条件下对R-PE的各亚基进行了分离纯化并对其各亚基可见光光谱特性作了研究。

Network advertisement than other media the largest dominant position is interactive sex, easily enterprise and consumer establish one-to-one sale relationship, the association between person of no less than and person carries each other communication and channel an versatile person only can promotional each other concern is same, interactive means makes advertisement is mixed by the conduct propaganda that sets out from company point of view so homiletic the communication that turns into to set out from consumer angle and communication, increased a target to suffer greatly numerous pair of advertising welcome degree and accept rate.

网络广告较之其它媒体最大的优势便是互动性，易于企业与消费者建立一对一的营销关系，正如人与人之间的交往只有通过相互的交流与沟通才能增进彼此的关系一样，互动方式使广告由原来从企业角度出发的宣传和说教变为从消费者角度出发的沟通与交流，大大增加了目标受众对广告的欢迎程度和接受程度。

Common transparent quartz tube SiO2 more than 99.9% purity, geometry, chemical stability, thermal stability, resistance to thermal denaturation and anti-crystallization properties of the fine, optical transmittance above 93%; from hydroxyl in hydroxyl tube below 10ppm, after vacuum from hydroxyl, hydroxy-up to 5ppm below apply to high-pressure mercury lamp, sodium lamp, metal halide lamp, such as low-hydroxy products; white pipe, used mainly for heating tube, heating tube, far infrared control the manufacture, milky quartz pearl quartz tube possession of new technology, the management side as if the surface of pearls, all aspects of performance than ordinary white tube better; filter tube to filter out ultraviolet UV band in the harmful wavelengths of 190-320nm ultraviolet light source applied to environmental protection manufacturing; no ozone UV filter tube and pipe the contrary, through the ultraviolet wavelength around 253.7nm, there is no ozone UV lamp main material, widely used in medical equipment, water treatment, food hygiene, such as sterilization; quartz casing diameter 8-120mm in between, has a single opening, double opening round, flat-top shape and widen the mouth of necking, flanging, sanding and other processing, high temperature resistance, corrosion resistance, good light transmission and moisture-proof, explosion-proof characteristics such as widely used in aquarium thermostat, disinfection equipment, heating pipes, water treatment, metal halide lamp, such as product manufacturing and environmental protection, health, chemical and other fields; quartz Clamshell specifications 8-180mm, the shape according to the customer requirements, the bubble shell can take capillary has to carry out the exhaust, high temperature, light transmission is good, mainly for JC, JD, H1-H7, the stage lights and high-pressure mercury lamp, sodium lamp, metal halide lamp, such as product manufacturing; quartz water jacket mainly used in printing and packaging processing industry ---- UV light-curing, UV light curing is a very reliable and can be used to replace hot air dryers and other drying technique of curing technology, which in the printing and packaging of the processing industry has been widely.

普通透明石英管SiO2纯度达99.9%以上，几何尺寸、化学稳定性、热稳定性、抗热变性和抗析晶性能优良，光学透过率达93%以上；脱羟管羟基在10ppm以下，经过真空脱羟，羟基可达5ppm以下，适用于高压汞灯、钠灯、金卤灯等低羟基产品；乳白管，主要用于发热管、加热管、远红外管的制造，珠光石英管乳白石英管之新工艺，管面好象珍珠的表面，各方面性能比普通乳白管更优；滤紫外线管能够滤除紫外光谱波段中对人体有害的190-320nm波长的紫外线，适用于环保光源的制造；无臭氧管与滤紫外线管相反，可透过253.7nm左右波长的紫外线，是无臭氧紫外线杀菌灯的主要材料，广泛用于医疗器械、水处理、食品卫生等杀菌；石英套管直径范围在8-120mm之间，有单开口、双开口、圆头、平头等外形和扩口、缩口、翻边、磨砂等加工，具有耐高温、耐腐蚀、透光性好及防潮、防爆等特点，广泛使用于水族恒温器、消毒设备、加热管、水处理、金卤灯等产品的制造和环保、卫生、化工等领域；石英泡壳规格Φ8-180mm，形状根据客户要求，泡壳上可以接有毛细管，可进行排气，耐高温，透光性好，主要用于JC、JD、H1-H7、舞台灯和高压汞灯、钠灯、金卤灯等产品的制造；石英水套主要用于印刷和包装加工工业---- UV光固化， UV光固化是一种十分可靠的、可取代热空气干燥机和其他干燥技术的固化技术，它在印刷和包装加工工业已经得到广泛应用。

It has been put forward that there exists single Ball point and double Ball points on the symmetrical connecting-rod curves of equilateral mechanisms.

从鲍尔点的形成原理出发，分析对称连杆曲线上鲍尔点的产生条件，提出等边机构的对称连杆曲线上有单鲍尔点和双鲍尔点。

The factory affiliated to the Group primarily manufactures multiple-purpose pincers, baking kits, knives, scissors, kitchenware, gardening tools and beauty care kits as well as other hardware tools, the annual production value of which reaches US$ 30 million dollars.

集团所属工厂主要生产多用钳、烤具、刀具、剪刀、厨具、花园工具、美容套等五金产品，年生产总值3000万美元，产品价廉物美、选料上乘、质量保证，深受国内外客户的青睐