查询词典 osteoplastic

Methods:Immunohistochemistry and nonradioactive in situ hybridization methods were used to detect the expression of p27mRNA and protein in 58 cases of osteoplastic tumors.

采用免疫组化和非放射性核酸原位杂交技术检测成骨性肿瘤中p27蛋白和mRNA的表达状况。

Objective:To evaluate the significance of p27mRNA and protein expression in various kinds of osteoplastic tumors.

目的：探讨p27mRNA与蛋白在成骨性肿瘤中的表达状况，了解其与肿瘤组织学类型、分化程度及生物学行为的关系。

Cell growth curve was also drawed before and after differentiation. When cultured in the osteoplastic induction medium, ADSCs derived epithelial cells might further differentiate into osteoblasts.

在成骨细胞诱导培养基中进一步诱导ADSCs源表皮细胞向成骨细胞定向分化，对诱导后的细胞进行ALP染色和Yon Kossa染色检查。

Spindle shape of cells gradually disappeared, with increased cell body. Some cells grew in cluster. MSCs changed figures to multilayer knots 10 days after inducing osteoplastic differentiation.

骨髓间充质干细胞向成骨分化过程中，纺锤形的突起逐渐消失，胞体增大，培养10 d后，部分细胞呈聚集生长，随细胞生长密度的增长形成多层的结节结构。

MAIN OUTCOME MEASURES: Morphological changes in primary cultured and subcultured mouse BMSCs were measured. BMSC surface antigen of the second passage of mice (CD105, CD44, CD25, CD34) were determined using flow cytometry. The modified method was assessed to harvest the purity of stem cells. Activity of mouse BMSCs was identified using adipogenic and osteoplastic differentiation. The strength of fluorescent cells following multiple passage was observed.

主要观察指标：原代及传代小鼠骨髓间充质干细胞形态变化；对第2代小鼠骨髓间充质干细胞表面抗原，CD105，CD44，CD25，CD34进行流式细胞仪检测，评价改良方法获取干细胞纯度；通过成脂成骨分化，鉴定小鼠骨髓间充质干细胞的活性；观察多次传代后荧光细胞强度。

RESULTS: High-purity ADSCs were acquired successfully. The ADSCs were induced into osteoplastic differentiation, and displayed typical adipocytes changes 7 days after induction. the cells turned to slender fusiform and mixed together, which showed positive PPARγ immunohistochemical staining and the lipid droplet was stained orange-red by oil red staining.

结果：①成功获得纯度较高的脂肪基质细胞，成功诱导其脂向分化，诱导培养后7 d观察到细胞形态学上表现出典型的脂肪细胞改变：细胞相互融合，成片生长，变为细长纺锤形；PPARγ免疫细胞化学染色呈阳性；油红O染色脂滴被染成橙红色。

After osteoplastic induction, peripheral blood MSCs had strongly positive reactions for alkaline phosphatase staining, total collagen staining, alizarin red staining.

外周血间充质干细胞成骨诱导后，碱性磷酸酶染色、总胶原染色及茜素红染色均呈强阳性；成脂诱导后油红染色呈阳性，有一定数量的脂滴形成。

On the other hand, the more important thing is because the urban housing is a kind of heterogeneity products.

另一方面，更重要的是由于城市住房是一种异质性产品。

Climate histogram is the fall that collects place measure calm value, cent serves as cross axle for a few equal interval, the area that the frequency that the value appears according to place is accumulated and becomes will be determined inside each interval, discharge the graph that rise with post, also be called histogram.

气候直方图是将所收集的降水量测定值，分为几个相等的区间作为横轴，并将各区间内所测定值依所出现的次数累积而成的面积，用柱子排起来的图形，也叫做柱状图。