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The asymbiotic germination indicated that the medium Hyponex No.1 was optimum for PLB formation when supplemented with potato juice, and optimum for PLB differentiation when supplemented with banana juice. Fresh sprouts were induced from the explant of the shoot tip cultured on the MS medium in the presence of BA 3.0 mg/L and NAA 0.2 mg/L.

结果表明：在花宝1号附加土豆的培养基上萌芽效果最好，原球茎发生率较高；分化阶段以花宝1号附加香蕉的培养基分化效果最理想，幼嫩茎尖在MS基本培养基上附加BA 3.0 mg/L与NAA 0.2 mg/L上可诱导出新芽。

Is a small freshwater alga suitable to live in an environment where both water temperature and light intensity is relative low. The optimum water temperature of 15℃ and the optimum light intensity of 35μmol/m^2s~45μmol/m^2 s are needed for photosynthesis of Batrachospermum longipedicellatum.

其光合作用的最适水温为15℃，光合作用的适宜光强为30μmol/平方公尺s~50μmol/平方公尺s，最适光强度为35μmol/平方公尺s~45μmol/平方公尺s，40μmol/平方公尺s光合速率最高峰，为其饱和光强。

So the optimum treating temperature to bivoltine races was from 44～46℃. When unfertilized eggs were treated with hot water for 3～68 minutes, asexual developed eggs could be obtained. But the optimum soaking time range to bivoltine races was 14～ 16 minutes.

针对我国的二化性家蚕现行品种，其无性繁殖诱导温度以44℃~46℃为宜；无性繁殖的处理时间适应范围较广，从3min~68min都可获得一定比例的无性繁殖发育卵，我国的二化性家蚕现行品种，其无性繁殖处理时间以14~16min为宜。

The effects of solvent on the bromization reaction and oxidant on the formolation reaction of the target product were studied. The results show that the optimum solvent for the bromization reaction and the optimum oxidant for the formolation reaction of the target product were carbon tetrachloride and ceric ammonium nitrate, and their yields were 86.7% and 89.3% respectively.

同时就溴化反应中的溶剂以及甲酰化反应中的氧化剂对两类反应的影响分别进行了研究，结果表明溴化反应的最佳溶剂为四氯化碳，反应产率达到86.7%，甲酰化反应的最佳氧化剂为硝酸铈铵，产率达到89.3%。

The optimum temperature s and pH were(40℃,2.4)for stomach,(55℃,7.6) for pyloric caeca(45℃,8.0) for gut. The optimum temperature for protease activity was higher than that in the fish body. The highest activity of protease occurred in strong acidic condition in stomach,but in neutral or weak alkaline condition in pyloric caeca and gut.

蛋白酶的活力随温度和pH变化呈现一定的规律性，不同消化器官蛋白酶的最适温度、pH依次为胃(40℃，2.4)、幽门盲囊(55℃，7.6)、肠(45℃，8.0)，蛋白酶的最适温度高于体温，胃蛋白酶在强酸环境下活力最大，幽门盲囊与肠在中性和偏碱条件下活力最大

Optimum nutritional level of concentrated feeds of adult capreolus: optimum protein level of concentrated feeds of adult male capreolus in service period was 23.73%,concentrate of energy was 53.69g. To overwintering and normal development, adult male capreolus in service period needed digestible protein 53.69g, digestible energy 38.03MJ per day.

成年狍精饲料适宜营养水平：成年公狍配种期精饲料较适宜的蛋白质水平为23.73％、能量浓度为17.19，为保证公狍安全越冬及狍正常发育，成年公狍配种期每天需要供给可消化蛋白质53.69g，可消化能38.03MJ。

Results indicate that the optimum extraction conditions are as follows: ultrasonic power is 600W, temperature is 53℃, the ratio of liquid to solid is 8︰1, ultrasonic treating time is 70min, reflux extraction time is 120min, the extraction rate of oleanolic acid is 3.000 mg/g; XDA-1 resin is favorable in purification of OA in Chaenomeles sinensis Koehne, the optimum purification conditions are found to be 0.794 mg/mL of OA concentration, the flow rate of adsorption is 0.5 mL/min; the desorption solvent is 90% ethanol, and the flow rate of elutant is 1.0 mL/min.

结果表明，超声波辅助提取最佳工艺条件为：超声功率600 W、提取温度53℃、液料比8：1、超声波提取70 min，回流提取120 min，齐墩果酸得率为3.000 mg/g；XDA-1型大孔树脂对齐墩果酸具有较好的吸附和解吸效果，最佳纯化工艺条件为上柱液浓度0.794 mg/mL，吸附流速0.5 mL/min；以90%浓度乙醇洗脱，洗脱流速1.0 mL/min，纯化后得提取物中齐墩果酸纯度可达26.55%。

Selecting the optimum carbon sources, nitrogen sources and inorganic salt to get the optimum liquid medium recipe by the orthogonal test in three factors and four levers; Isolated toad gastrocnemius stimulated by pulse electricity was regarded as fatigue model and Clitocybe maxima mycelia was compared with Ringer solution to see what influence they had on muscle contract ability.

采用深层培养方法，筛选出较优的碳源、氮源、无机盐，设计三因素四水平的正交试验，筛选大杯伞的最适培养基；采用脉冲式电流直接刺激蟾蜍离体腓肠肌作为疲劳模型，观察大杯伞菌丝体和任氏液对蟾蜍腓肠肌收缩能力的影响。

Synthesis of Antidote of N-Dichloroacetyl-2-methyl-1-oxa-4-aza-spiro-4.4-nonane BI Hong-mei, ZHANG Jin-yan, LIANG Ying, SHEN Yong (College of Arts and Sciences Heilongjiang August First Reclamation University, Daqing 163319, Heilongjiang, China) Abstract: N-Dichloroacetyl-2-methyl-1-oxa-4-aza-spiro-4.4-nonane was synthesized by one pot method with isopropanolamine, cyclopentanone and dichloroacetyl chloride. The optimum reaction conditions obtained are as follows:the proportion in moles of the above materials is 1∶1∶1.2, reaction solvent is toluene, reaction temperature is -15 to -10 ℃,reaction time is 3 h, under the optimum condition, the yield of the compound is 50.0%.

除草剂解毒剂N-二氯乙酰基-2-甲基-1-氧杂-4-氮杂-螺[4.4]壬烷的合成毕洪梅，张金艳，梁英，沈勇(黑龙江八一农垦大学文理学院，黑龙江大庆 163319)摘要：以33%的氢氧化钠水溶液为缚酸剂，异丙醇胺、环戊酮和二氯乙酰氯为原料，采用&一锅法&合成了N-二氯乙酰基-2-甲基-1-氧杂-4-氮杂-螺[4.4]壬烷，采用正交实验法得到最佳反应条件：反应原料摩尔比为1∶1∶1.2，苯作溶剂，反应的温度为-15 ℃~-10 ℃，搅拌时间为3 h，产率达到50.0%。

The optimum formula was as follow: the fresh milk and emblic leafflower fruit juice mixed according to the proportion of 8:1, and 8.5% sugar were added; optimum compound stabilizers: starch 0.10%+pectin 0.025%+PGA0.05%; amount of latic acid bacterium: 5%; fermenting temperature: 42℃; time of fermentation: 2.5h.

结果表明，余甘子酸乳的最佳配比为：鲜牛乳与果汁比例8：1、蔗糖含量8.5%，复合稳定剂为淀粉0.10%+果胶0.025%+PGA0.05%。最佳发酵工艺条件为：接种量5%、滴定酸度76~80°T、发酵温度为42℃、发酵时间为2.5h。

They weren't aggressive, but I yelled and threw a rock in their direction to get them off the trail and away from me, just in case.

他们没有侵略性，但我大喊，并在他们的方向扔石头让他们过的线索，远离我，以防万一。

In slot 2 in your bag put wrapping paper, quantity does not matter in this case.

在你的书包里槽2把包装纸、数量无关紧要。