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Results: The optimal concentration of Luceferase and Luciferin were 1.25ug/ml and 87.5uM; Reaction solution was prepared according to optimal concentration. From the ATP standard curve the lowest ATP test limit was found to be , the H1299 lowest test limit was 100 cells.

结果:Luciferase和Luciferin最佳反应浓度分别为1.25ug/ml和87.5uM;按照最佳浓度配制反应液后,绘制ATP浓度标准曲线,找到ATP检测最低线为10-12mol/L,可以检测到H1299细胞最低值为100个。

Methods: In accordance with conventional reporter gene analysis reagent preparation method, the main component Luciferase, Luciferin and ATP were serial diluted to find the optimal concentration; Reaction solution was prepared according to the optimal concentration of the main component, then ATP were serial diluted to measure their reporter gene. Then standard curve of ATP were drawed. Tumor cell lines H1299 were serial diluted and their ATP were measured by reaction solution prepared to analyse the minimum cell number.

按照常规报告基因分析试剂配制方法,将其中主要成分Luciferase和Luciferin及ATP浓度进行倍比稀释后找到最适合反应的最佳浓度值;按照最佳浓度配制反应液,将ATP浓度倍比稀释测定报告基因数值并绘制标准曲线,分析检测灵敏度;另外选取肿瘤细胞株H1299,倍比稀释后裂解细胞应用配制好的反应液进行ATP检测,分析最低检测细胞数。

The major contributions of this thesis include:(1) proposing a new kind of optimal feedback control, followed by its application to chaos targeting and stabilization of high period unstable periodic orbits ;(2) stabilization of unstable fixed point in chaotic systems using some kind of nonlinear feedback control, and giving sufficient condition for the existence of the controller as well as the synthesis procedure;(3) analyzing the robustness of optimal control in chaotic Lur'e systems under the help of H〓 control theory;(4) finally and most importantly, introducing a novel method of information storage based on discrete time chaotic systems as the application of chaos control, and systematically studying different schemata for implementation, providing a theoretic basis for the design of such new kind of information storage devices with large capacity.

本文的主要工作包括,提出一种新的最优反馈控制,并将之应用于混沌引导控制及混沌系统高周期不稳定周期轨道的镇定控制;针对不动点镇定控制的一类非线性反馈控制器,文中给出该类反馈控制存在的充分条件及设计步骤;文中还利用非线性H〓鲁棒控制理论对Lur'e混沌系统中的最优线性反馈控制进行鲁棒性分析;最后,也是最重要的,作为混沌控制的应用,文中提出一种基于离散时间混沌系统的信息存储方法,系统地分析、研究了实现这种信息存储的不同方案,为以后进行这种新型大容量存储器设计提供了一定的理论依据。

Based on the conception of the equivalent stress,the expressions of the equivalent stress was educed. In this optimal design, the macro file for calculating the equivalent stress of arch dam is complied by using the parameter design language of the software, and the optimal design for shape of arch dam using equivalent stress as stress restraint condition is carried out.

本文基于等效应力概念,推导了拱坝有限元等效应力计算公式,将有限元法求得的坝体与基岩交接面上的应力合成截面内力,然后求出对应的线性化应力,并以此应力作为应力约束条件,实现了以等效应力作为应力约束条件进行拱坝体形优化。

The optimal temperature of D6 is 30℃, the optimal pH ,7.0. The growth of D6 makes use of glucose, malt sugar, lactose, cane sugar, lactic acid, ethanol, glycerin, glycine, but no acetic acid and n-butanol. It can grow in Na2SO4, Na2SOa, Na2S2O3 and S media, and Na2S2O3 is the best substrate.

该菌最适生长温度为30℃,最适pH值为7.0,生长利用葡萄糖、麦芽糖、乳糖、蔗糖、乳酸、乙醇、甘油、甘氨酸,不利用乙酸和正丁醇;可以在Na_2SO_4、Na_2SO_3、Na_2S_2O_3和单质S培养基中生长,并以Na_2S_2O_3为底物时生长效果最佳。

According to the robust control H2 optimal performance specification, the practically desired diagonal elements of the system transfer matrix are suggested, and in view of the coupling action existing between the two closed loops, dynamic detuning factors are introduced to design the optimal double-loop controllers, and their executable PI/PID forms are derived using a mathematical rational approximation approach.

根据鲁棒控制H2最优性能指标,提出实际期望实现的系统传函矩阵中的对角元。考虑到两个闭环之间的耦合作用,引入动态解调因子设计最优双回路控制器,采用数学有理逼近的方法得到其实际可执行的PI/PID形式。

The optimal design of layout programming of RRMS is explored. The mathematical programming model based on matroid is established and the greedy method is selected serving as the optimal method. The application program based on this method is also developed and compiled.

摘 要:研究探索了快速可重组制造系统布局规划中的优化设计问题,建立了基于拟阵的数学规划模型,通过对比选取贪馋算法作为优化算法,并编制开发了此算法的应用程序。

As independent economic entities, the enterprises of the Supply Chain pursue maximal profit all the time, the buyer need to choose an optimal ordering strategy, and the supplier need to choose an optimal quantity discount.

供应链成员企业作为独立的经济实体,追求自身利益最大化,需求方考虑采取何种订货策略以降低成本,获得最大利润;供应方需要做出采取何种批发价格策略的决策,以使自己的收益最大。

The ratio of stock right calculated according to venture investment experiential principles is sound and easy to accept. It can give quantitative analysis support to the venture investment practice.(6)According to the theory of the real option, the author derived value formula of the real option fixed price and investment criterion. Based on above, the paper further research for optimal investment strategy of real option in monopolizing and competition under asymmetry information, and built the value model of real option for the investor and administrator by using the theory of the principle trust-agent Meanwhile, the solution of real option optimal investment and the shift value are derived applying the maximal principle .

针对高新技术企业发展的特点:研制周期长、投资费用高、每阶段风险水平特征差别大,运用实物期权理论与计算方法,通过研究建立嵌入期权现金流量图深入分析计算了企业发展中风险投资的最优策略、股权占有比例问题,对不确定性问题由于采用了有关实物期权计算公式,所以计算结果比基于传统的现金流量图的NPV方法更加客观、科学,同时按照风险投资经验法则计算出的股权占有比例合理易于接受,可为企业风险投资实践活动提供数量分析支持。

The so-called maximum principle is the necessary condition for an optimal control, one of the most principal and most commonly used approaches when facing a optimal control problem.

最大值原理,即最优控制满足的必要条件,是处理最优控制问题时最重要也是最常用的方法之一。

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