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We optimized reaction system for labelling-antibody in which the optimal amount of the purified anti-Stx2B IgG conjugated with colloidal gold beads was 60μg/mL, the purified antibody for E.coli O157 was 57μg/mL, the optimal pH was 8.2, the size of colloidal gold partical was 20nm, the optimization of stabilizing agent was BSA, the optimization buffer was boracic acid buffer with pH 8.2, the optimization of preserving fluid and eluant was boracic acid buffer with pH 8.2 including 5mM NaCl-1%BSA, confining liquid for NC membrane was 0.01% PBST with 3%BSA, the amount of polyclonal antibody against E.coli O157 and Stx2 conjugated with colloidal gold beads for conjugate pad was 3μg respectively, the amount of anti-Stx monoclonal antibody for test line was 0.1μg and 1μg for E.coli O157, the amount of goat anti rabbit IgG for control line of both GICA were 1μg.

测定了胶体金颗粒的最优化反应体系：胶体金颗粒的大小为20nm；抗体与胶体金溶液结合的最佳pH约为8.2；最佳蛋白结合量分别为抗大肠杆菌O157 IgG为57μg/mL，抗重组Stx2B IgG为60μg/mL；最佳稳定剂为BSA；最佳缓冲液为pH8.2硼酸溶液；最佳金标保存液和洗涤液为5mM NaCl-1%BSA的pH 8.2的硼酸缓冲液；NC膜的封闭液为3%BSA的0.01mol/L PBST；Stx2试纸条和大肠杆菌O157试纸条的质控线上的羊抗兔IgG的多克隆抗体最佳点样量均为1μg，其检测线的抗Stx2的单克隆抗体的点样量为0.1μg、抗大肠杆菌O157的单克隆抗体的点样量为1μg，结合垫上的金标抗大肠杆菌O157和重组Stx2B多克隆抗体点样量均为3μg。

The given Branch and Bound method can find out the optimal solution of the problem in small size while the given GA implement can quickly find out the approximate optimal solution of the problem in large size. The given GA implement proves to be very effective by numerical experiments and examples.

对于问题的较小规模情形分支定界法能精确地求得最优解；对于问题的较大规模情形遗传算法能很快地求得近似最优解；算例及大量实验表明用遗传算法来求问题的近似解是成功的。

The best ratio of brine shrimp and soybean-koji,the optimal temperature and the optimal salinity was determined with orthogonal experiment and Sensory evaluation to analyze.

利用正交试验并通过感官评定分析得出卤虾和豆曲的最佳混合比例，发酵所需的最适温度以及最适盐度。

In this paper, constrained optimal problems are solved using particle swarm optimization. Owing to constrained conditions, searching space is not bulgy and particles are easy to be limited to local optimal.

采用粒子群算法处理约束优化问题时，由於约束条件使得解空间成为非凸集合，粒子容易陷入局部最优。

The studies on biological characteristic revealed that the optimal temperature for the mycelial growth was 25~30℃, and the optimal pH for mycelial growth was pH 5~7. Among carbon and nitrogen sources, sucrose and calcium nitrate were best for mycelial growth. The lethal temperature for mycelial growth and spores gemination were respectively 55℃ and 60℃ for 10 min.

affinis引起草坪型高羊茅叶斑病在我国的首次报道；生物学特性的研究表明，该菌菌丝生长的最适温度为25~30℃，最适pH范围为6~7，最适碳源为蔗糖，最适氮源为Ca(NO3)2；菌丝和分生孢子的致死温度分别为55℃ 10 min和60℃ 10 min。

The analysis of our model shows that the overlapping stage reduces the time-to-market to some extent. The existing product with high performance contributes to the rduction of time to market, but not obviously; cannibalization rate wakens the ralation between the productivity of the overlapping stage and the optimal time-to-market, and the optimal duration of the overlapping stage respectively.

模型分析表明，重叠阶段只是在一定程度上缩短了新产品开发时间；基本产品性能的提高有助于缩短新产品的开发时间，但效果并不明显；成长率弱化了重叠阶段生产率与最优产品开发时间及重叠量之间的相关性。

Cannibalization rate wakens the ralation between the productivity of the overlapping stage and the optimal time-to-market, and the optimal duration of the overlapping stage respectively.

成长率弱化了重叠阶段生产率与最优产品开发时间及重叠量之间的相关性。

Applying queuing theory to inventory control,it established an optimal inventory model of a production system with Poisson process about rate of demand and service times,and obtained the optimal inventory capacity for the production system.

将排队论方法用于存贮问题中，建立了需求的到达与产品的生产均服从Poisson过程的生产系统的最优存贮模型，求得了最优库存容量。

The cartel that is optimal for its members is not optimal for the world, of course.

诚然，对其成员国来说是最优的卡特尔，对世界来说未必是最优的。

The algorithm GS to solve the problem of inverse dynamics based on the optimal theory is proposed, by using the numerical methods of Gill and shooting target, the optimal joint driven torques are obtained With the smooth joint trajectory motion, a fast and stable inverse dynamics method is proposed, it provides a new effective way to control the flexible manipulator The trajectory planning in the joint space and Cartesian coordinates is especially discussed In terms of kinematics of space flexible link manipulators, a sequential integration method for inverse dynamic analysis of flexible manipulators is developed The concrete results of simulations tested and verified the validity of proposed methods, and some valuable conclusions are achieved.

提出了求解基于最优理论的柔性臂逆动力学问题的GS法(Gill—Shooting method)，得到了柔性臂关节的最优驱动力矩；提出了基于光滑关节运动轨迹的柔性臂逆动力学的快速稳定方法，为柔性臂控制提供了一条新的途径；探讨了关节空间和直角坐标空间柔性臂的轨迹规划问题；研究并完善了结合柔性臂运动学的顺序积分的逆动力学方法。通过具体实例的仿真研究，验证了所提出的柔性臂逆动力学方法的有效性，得出了一些很有价值的结论。

They weren't aggressive, but I yelled and threw a rock in their direction to get them off the trail and away from me, just in case.

他们没有侵略性，但我大喊，并在他们的方向扔石头让他们过的线索，远离我，以防万一。

In slot 2 in your bag put wrapping paper, quantity does not matter in this case.

在你的书包里槽2把包装纸、数量无关紧要。