查询词典 operation expression

Results 1.VEGF-C expression and MVD in epithelial ovarian carcinoma were higher than those in normal ovarian tissue (P.05),TSP-1 expression were lower than those in normal ovarian tissue (P.05);2.In epithelial ovarian carcinoma tissue,VEGF-C expression and MVD increasing according to progression of the FIGO phase but TSP-1 expression decreasing according to progression of the FIGO phase.

结果 卵巢上皮癌中VEGF-C表达及MVD均明显高于正常卵巢组织(P.05)，TSP-1表达低于正常卵巢组织(P.05)；卵巢上皮癌组织中，VEGF-C表达及MVD随国际妇产科联盟分期的进展而增高(P.05)，TSP-1表达随FIGO分期的进展而降低(P.05)。

In addition, we presume that the role of different MMPs is distinct in various stages of EMs development. The difference of subjects which is resulted by the genetic difference of MMP-1 has the association with the risk of EMs, and the abnormal expression of protein may have relation with the development of EMs; The abnormal expression of MMP-3 has a very important role in the development of EMs; As a protein of endothelial expression, MMP-7 may participate in the development of EMs, endometrial cells with high-expression MMP-7 easily take place ectopic implantation in the blood of menstruate countercurrented and increase the risk of EMs; It is not confirmed in our study that there are the association of MMP-9 with the risk of EMs, but in view of other study, we will further evaluate the role of MMP-9 in the development of EMs.

且推测不同的MMPs基因在子宫内膜异位症发生和发展的不同阶段作用不同，MMP-1基因的遗传差异所导致的个体差异与子宫内膜异位症发病风险的个体差异相关，其蛋白的异常表达还可能与子宫内膜异位症的进展相关；MMP-3基因的异常表达在子宫内膜异位症的发展中起重要作用；MMP-7作为一个上皮性表达的蛋白，可能对子宫内膜异位症的发生起重要作用，带有MMP-7高表达的在位子宫内膜细胞更易在经血逆流中"异位"种植，增加个体子宫内膜异位症的发病风险；MMP-9虽然在本研究中没有被证实与子宫内膜异位症的发生和发展有相关性，但鉴于其它相关研究，有待于从其它方法证实MMP-9在子宫内膜异位症进展中的作用。

We study the expression levels of GLUT1 in 16 samples of esophageal cancer and their normal mucosa by reverse transcriptasepolymerase chain reaction and immunohistochemical staining, analyse the correlation between the expression level of GLUT1 mRNA and protein in the tumor cells. Then we study the expression levels of GLUT1 in 55 patients with esophageal cancer by immnohistochemical staining, analyse the relationship between GLUT1 expression and clinical features of the patients.

我们采用逆转录聚合酶链反应和免疫组化染色的方法，研究GLUT1 mRNA及蛋白在16例食管癌病人的肿瘤组织和相应正常食管粘膜的表达水平，同时分析食管癌组织中GLUT1在mRNA水平和蛋白水平表达的相关性；采用免疫组化染色的方法研究55例食管癌病人肿瘤标本的GLUT1表达水平，并分析其与肿瘤的临床特性及预后的关系。

Although both of FDRMADS7 and FDRMADS8 wereexpressed in the root, they have different expression pattern.The transcripts of FDRMADS7 weredetected throughout the root,especially the expression in the root meristem was the highest.However,the expression of FDRMADS8 was mainly localized in elongation and mature rigion.No apparent expression signal was detected in the root cap, root meristem and pericycle cells.

虽然FDRMADS7和FDRMADS8都在根中表达，但它们在根中的表达模式也不相同：FDRMADS7在根中到处表达，尤以生长点处表达量最高；而FDRMADS8在伸长区和根毛区的皮层中有大量表达，在根冠，根尖分生区和根毛区的中柱鞘细胞中却无明显表达，暗示它们在根中行使的功能也不相同。

Under different stresses, we tested the expression of citrate synthase gene in rapeseed leaf by using semi-quantitative PCR. The expression of citrate synthase gene had no obvious change in stresses of salt, dark, high illumination, while was increased at different time in treatments of water logging, drought, IAA, and 6-BA. Interestingly the effect of ABA was contrary to that of IAA. In the treatment of sclerotium blight, the expression of citrate synthase gene was depressed. There was a saddle curve of citrate synthase gene expression in the treatment of gibberellin.

对油菜幼苗进行植物生长调节物质、高温和低温、强光照和弱光照、盐、菌核病、干旱和水渍等处理，采用半定量PCR法对油菜叶片柠檬酸合酶基因的表达模式进行检测，发现在盐胁迫、暗光和强光的处理下，柠檬酸合酶基因的表达基本没有变化；在水渍、干旱、IAA和6-BA胁迫下，其表达有所升高，但出现峰值的时间不同，ABA对表达模式的影响与IAA相反，感染菌核病后其表达降低；对GA3的应答呈鞍型。

Known differential expression gene: In the 46 points of known differential expression gene, there were down - regulate gene expression 41 points and up- regulate gene expression 5 points; twenty - eight genes related to hypoxia/reoxygenation had not been reported and 18 genes had been reported.

已知差异表达基因：在46个已知差异表达基因中，表达下调的基因有41个，表达上调的基因有5个，未见报道的与缺氧/复氧处理相关基因23个，已报道的有18个。

The stable clones are further identified by RT-PCR and Western blot; 6 MTT assay is used to investigate the effect of ZNRD1 on the cell growth of cells (AGS, SGC7901, MKN28, NIH3T3, GES-1); 7 Soft agar assay is used to investigate the effect of ZNRD1 on the clonality of cells (AGS, MKN28); 8 Nude mice assay is used to investigate the effect of ZNRD1 on the cell growth of gastric cancer cells (AGS, MKN28); 9 Flow cytometry is used to investigate the effect of ZNRD1 on the cell cycle distribution of cells (AGS, MKN28, NIH3T3, GES-1); 10 Flow cytometry is used to investigate the effect of ZNRD1 on the cell apoptosis of cells (AGS, MKN28, NIH3T3); 11 MTT assay is used to investigate the effect of ZNRD1 on the drug sensitivity of cancer cells (SGC7901, SGC7901/VCR, HL-60, HL-60/VCR) in vitro; 12 SRCA is used to investigate the effect of ZNRD1 on the drug sensitivity of gastric cancer cells (SGC7901, SGC7901/VCR) in vivo; 13 Flow cytometry is used to investigate the effect of ZNRD1 on adriamycin accumulation of cancer cells (SGC7901, SGC7901/VCR, HL-60, HL-60/VCR); 14 Transmission electron microscope is used to investigate the effect of ZNRD1 on the sensitivity of SGC7901 cells towards drug-induced apoptosis; 15 Flow cytometry and DNA ladder assay are used to investigate the effect of ZNRD1 on the sensitivity of cells (SGC7901, SGC7901/VCR, HL-60/VCR) towards drug-induced apoptosis; 16 Microarray is used to investigate the profiling of ZNRD1-responsive genes in gastric cancer cells (AGS, MKN28, SGC7901, SGC7901/VCR); 17 RT-PCR and Western blot are used to identify the results of microarray; 18 Reporter gene assay is used to investigate the effect of ZNRD1 on the transcriptional activity of cyclin D1; 19 Reporter gene assay is used to investigate the effect of ZNRD1 on the transcriptional activity of MDR1; 20 Kinase assay is used to investigate the effect of ZNRD1 on the activity of cyclin E-CDK2 kinase; 21 The antisensenucleic acids of p21 is used to inhibit the expression of p21, and flow cytometry is used to investigate the effect of p21 on ZNRD1-induced cell cycle arrest in gastric cancer cells; 22 The antisensenucleic acids of p27 is used to inhibit the expression of p27, and flow cytometry is used to investigate the effect of p27 on ZNRD1-induced cell cycle arrest in gastric cancer cells; 23 Liposome is used to up-regulate the expression of Skp2, and flow cytometry is used to investigate the effect of Skp2 on ZNRD1-induced cell cycle arrest in gastric cancer cells; 24 Western blot is used to investigate the effect of ZNRD1 on the stability of Skp2 and p27 in gastric cancer cells; 25 MVD assay is used to investigate the effect of ZNRD1 on the angiopoietic activity of gastric cancer cells; 26 ELISA is used to investigate the effect of ZNRD1 on the expression of VEGF165 in gastric cancer cells; 27 The roles of DARPP-32 in MDR of gastric cancer cells are investigated using gene transfection, MTT assay, SRCA, flow cytometry and DNA ladder assay.

应用杂交瘤技术制备ZNRD1的首个单克隆抗体；2）利用RT-PCR、Western blot和免疫组化检测ZNRD1在胃癌组织、胃炎组织、正常胃上皮组织、胃癌细胞和正常胃组织上皮细胞中的表达；3）构建ZNRD1的小干扰RNA载体，并测序鉴定；4）利用脂质体将ZNRD1的真核表达载体及其空载体转染胃癌细胞（AGS、SGC7901、MKN28）和小鼠成纤维细胞（NIH3T3），G418筛选后进行鉴定；5）利用脂质体将ZNRD1的小干扰RNA载体及其空载体转染药敏胃癌细胞（SGC7901）、正常胃组织上皮细胞（GES-1）、对长春新碱耐药的胃癌细胞（SGC7901/VCR）、药敏白血病细胞（HL-60）、对长春新碱耐药的白血病细胞（HL-60/VCR），G418筛选后进行鉴定；6）利用MTT实验检测ZNRD1高/低表达对细胞（AGS、SGC7901、MKN28、NIH3T3、GES-1）生长的影响；7）通过软琼脂克隆形成实验检测上调ZNRD1对AGS、MKN28细胞克隆形成能力的影响；8）通过裸鼠成瘤实验检测上调ZNRD1对AGS、MKN28细胞体内成瘤性的影响；9）通过流式细胞仪分析ZNRD1高/低表达对细胞（AGS、MKN28、NIH3T3、GES-1）的细胞周期的影响；10）通过流式细胞仪分析上调ZNRD1对细胞（AGS、MKN28、NIH3T3）的凋亡的影响；11）通过MTT实验检测ZNRD1高/低表达对细胞（SGC7901、SGC7901/VCR、HL-60、HL-60/VCR）体外药物敏感性的影响；12）通过肾包膜下移植法检测ZNRD1高/低表达对细胞（SGC7901、SGC7901/VCR）体内药物敏感性的影响；13）通过流式细胞仪分析ZNRD1高/低表达对细胞（SGC7901、SGC7901/VCR、HL-60、HL-60/VCR）内阿霉素蓄积和泵出的影响；14）通过透射电镜检测上调ZNRD1对SGC7901细胞凋亡敏感性的影响；15）通过流式细胞仪和DNA梯度试验检测ZNRD1高/低表达对细胞（SGC7901、SGC7901/VCR、HL-60）凋亡敏感性的影响；16）通过基因芯片检测ZNRD1高/低表达对胃癌细胞内基因表达谱的影响；17）利用RT-PCR、Western blot对基因芯片的结果进行鉴定；18）利用报告基因实验检测ZNRD1对cyclin D1的启动子活性的调节作用；19）利用报告基因实验检测ZNRD1高/低表达对MDR1的启动子活性的调节作用；20）利用激酶试验检测ZNRD1对cyclin E-CDK2 激酶活力的影响；21）利用反义核酸技术抑制p21的表达；通过流式细胞仪检测抑制p21对ZNRD1介导的细胞周期阻滞的影响；22）利用反义核酸技术抑制p27的表达；通过流式细胞仪检测抑制p27对ZNRD1介导的细胞周期阻滞的影响；23）利用脂质体转染法上调Skp2的表达；通过流式细胞仪检测上调Skp2对ZNRD1介导的细胞周期阻滞的影响；24）利用Western blot检测ZNRD1对p27和Skp2的蛋白稳定性的影响；25）利用微血管密度实验检测ZNRD1对AGS、MKN28细胞裸鼠移植瘤微血管形成的影响；26）利用ELISA检测ZNRD1对AGS、MKN28细胞培养上清和移植瘤匀浆中VEGF165含量的影响；27）利用脂质体转染法、MTT实验、肾包膜下移植法、流式细胞仪和DNA梯度试验检测新耐药相关分子DARPP-32对细胞（SGC7901、SGC7901/VCR、对阿霉素耐药的胃癌细胞SGC7901/ADR）多药耐药表型的影响；利用脂质体转染法和MTT实验检测下调ZNRD1对DARPP-32介导的胃癌多药耐药的调控作用。

And proved that there is CBF gene expression-regulated pathway and transcriptional cascade leading to the expression of cold-responsive genes under cold stress in Capsella bursa-pastoris, which Cbice53 activate the expression of Cbcbf and Cbcbf activate transcription of Cbcor15b and the CbLos2 had a indirect and important regulatory function in controlling cold-responsive genes expression under low temperature like the same pathway in Arabidopsis thaliana.

证明了在荠菜中存在Cbice53激活Cbcbf的转录，Cbcbf激活Cbcor15b的转录，同时CbLos2对Cbcor15b的转录有间接的正向调控作用的抗冷胁迫基因表达级联机制，即存在与拟南芥相类似的抗冷胁迫的CBF基因表达调控途径。

Results］ the expression of cox-2 in hepatocellular carcinoma and its adjacent tissues with cirrhosis were 70.0% and 92.0% respectively, and they were higher than that in normal liver tissue (20.0%). the cox-2 expression in carcinoma adjacent tissues with cirrhosis was higher than that in hcc. expression of cox-2 in well differential hcc was higher than that in poor differential hcc, and higher in case with metastasis than that without metastasis(p＜0.01).the expression of cox-2 in hcc did not relate to tumor size, tumor capsula and the level of afp.

结果］ cox-2蛋白在hcc、癌旁肝硬化中表达阳性率分别为70.0%和92.0%，显著高于在正常肝组织中的20.0%（p＜0.05），而cox-2在癌旁肝硬化组织中的表达高于hcc组织。cox-2蛋白在分化好hcc中的表达显著高于分化差的hcc（p＜0.05），转移组中cox-2蛋白表达显著高于无转移组（p＜0.01）；而cox-2蛋白的表达与肿瘤大小、有无包膜及afp水平无关。

Results The levels of HSP 70 mRNA and HSP 70 protein expression were significantly different among the 5 groups. Ketamine induced marked HSP 70 mRNA and HSP 70 protein expression in the posterior cingulated cortex. Propofol itself did not induce HSP 70 gene expression in this brain region. Propofol significantly inhibited ketamine-induced HSP 70 mRNA and HSP 70 protein expression in the posterior cingulate cortex in a dose-dependent manner.

结果氯胺酮可明显诱导HSP70 mRNA与HSP70蛋白在大鼠后扣带回皮质区的表达；异丙酚自身不能诱导HSP70基因的表达；预先给予异丙酚可显著抑制氯胺酮诱导的HSP70 mRNA和HSP70蛋白在这一区域的表达，且抑制效应呈剂量依赖性。

Salt is good, but if the salt becomes flat and tasteless, with what do you season it?

14：33 盐本是好的，盐若失了味，可用什么叫它再咸呢？

He reiterated that the PLA is an army of the people under the leadership of the Communist Party of China.

他重申，人民解放军是在中国共产党领导下的人民军队。