查询词典 operation expression

objective: to achieve the soluble expression of mn-sod gene in e.coli and assay the enzyme activity of the expressed product. methods: the coding region of superoxide dismutase was amplified using pcr method from the e.coli genome. the pcr product was cloned into puc19-t vector and sequenced.in addition,the cloned coding region of mn-sod was inserted into the expression vector pet-28a to form the recombinant plasmid pet-28a-mn-sod and was then transformed into e.coli bl21 for expression.

超氧化物歧化酶(superoxide dismutase，sod)是细胞体内歧化超氧阴离子自由基(o-2)的一个抗氧化酶，按其结合的金属性离子根据其中金属辅基的不同可分为4类：cu/zn-sod、mn-sod、fe-sod和ni-sod，它们通过催化超氧阴离子自由基0-2发生歧化反应，达到清除o-2的效果，具有防御氧毒性、增强机体抗辐射损伤能力、防衰老以及治疗某些肿瘤、炎症、自身免疫疾病等功效，广受国内外科研工作者的关注和重视[1]。

To explore the possibility that the androgen may facilitate differentiation of hair follicle stem cells into sebocytes, we carried out experiments to test this hypothesis. The concrete steps to carry out this research were as follow: Firstly, observed the expression of AR and examined the expression of AR mRNA of rat vibrissa follicle stem cells in vivo in three defined phases of a hair follicle cycle, including anagen, catagen and telogen. Secondly, cultured HFSC deprived from vibrissa follicle bulge region in culture flasks and observed the expression of AR, ketertin 15 (K15), cluster of differentiation 34 (CD34) andβ1-integrin of those cells.

为了探索雄激素对毛囊干细胞定向分化为皮脂腺细胞的过程是否具有促进功能，我们进行了如下的实验来进行研究：第一步，检测了在毛囊周期的三个不同时期(生长期、退化期和静止期)中大鼠触须毛囊干细胞中AR、ARmRNA的表达以及检测原代培养的毛囊干细胞中AR的表达并探讨意义，为进一步研究打下基础。

Results With the expression in normal salivary gland as a standard, low C-erbB-2 mRNA expression was seen in adenolymphoma, basal cell adenoma. However, various degrees of over-expression of C-erbB-2 oncogene mRNA were detected in pleomorphic adenoma, mucoepidermoid carcinoma,acinic cell carcinoma ,adenoid cystic carcinoma ,papillary cystic carcinoma,and myoethelial cell carcinoma.

结果 正常涎腺和涎腺肿瘤中发现不同程度C-erbB-2mRNA的表达，以正常涎腺组织的表达水平为基准，在腺淋巴瘤、基底细胞腺瘤呈现低表达、无表达或与正常涎腺组织表达水平相似，多形性腺瘤、粘液表皮样癌、腺泡细胞癌、腺样囊性癌、乳头状囊腺癌和肌上皮癌则呈现不同程度的过表达。

Here we summarize the progress of salmon calcitonin's heterogenous expression in prokaryotic and eukaryotic expression systems as well as the amidation of C-terminal. Both the biological activity of salmon calcitonin and the biologic expression system can be improved by genetic engineering techniques.

本文综述了鲑鱼降钙素基因在大肠杆菌、链霉菌、乳酸杆菌和蓝藻等原核细胞，在酵母、昆虫、动植物等真核细胞中异源表达的研究进展，以及通过基因工程的方法能够改进生物表达系统，提高鲑鱼降钙素活性的动态。

Get high purity DCs by Cultured plastic-adherent monocytes isolated from healthy human peripheral blood with GM-CSF and IL-4 for 7 days. To observe the morphology of DCs by inverted phase contrast microscope ,electron microscope and laser confocal microscope. Analyse phenotype of DCs with flow cytometry. Investigate the endocytosis ability of DCs as a group by Horseradish peroxidase endocytosis assay. To appraise allogeneic mixed lymphocytes reaction of DCs by MTT reduction assay. Analyse the levels of IL-12 and TNF in liquids of cultured medium by ELISA and MTT reduction assay respectively. Soluble antigens of HCCs was obtained by 3 freeze-and-thaw cycles. Biological characteristics of HC soluble antigens pulsed DCs were monitored by flow cytometry. According to MTT reduction assay estimated the cell proliferation of self lymphocytes activated by HC antigens pulsed DCs. Get high purity BCG HSP 70 protein by SDS-PAGE electrophoresis and determined its biological activity with ELISA. Analyse phenotype of antigen pulsed DCs primed by BCG HSP70 with flow cytometry. By MTT reduction assay estimated the cell proliferation of self lymphocytes and the MLR of DC based vaccine. Analyse expression of HLA-DR molecule on surface of HCC lines. The IFN-γ mRNA in lymphocytes after actived by DC vaccine and the Fas-L expression on DC and DC vaccine primed lymphocytes were detected by in situ hybridization and flow cytometry respectively. Specific cytotoxity lysis of T lymphocytes and nonspecific inhibition of liquids in culture medium against HCC lines were also tested. Detect expression of hAFP on four HCC lines with Cell-ELISA. Induce apoptosis of HCCs with actinomycin-D. Interaction of DCs and apoptotic cells was observed under transmission electron microscope. Growth inhibition test of DC against HCC lines was also performed. Establish the nude mouse model bearing human HC xenografts and indentify the characteristic of tumour by histochemistry and immunohistochemistry techniques. Prevent and treat transplanted human HC on nude mouse with Freezing and anabiotic HC specific lymphocytes.

用GM-CSF和IL-4从健康人外周血诱导DC；分别用倒置相差显微镜、电子显微镜及激光共聚焦显微镜观察DC形态；流式细胞术检测DC表型；HRP吞噬实验测定DC的群体内吞能力；MTT法检测同种异体混合淋巴细胞反应；ELISA法和MTT法分别测定DC培养上清液中IL-12和TNF水平；冻融法制备肝癌细胞可溶性抗原；流式细胞术检测负载肝癌可溶性抗原后DC的生物学特性；MTT法检测DC负载肝癌抗原后对自身淋巴细胞增殖的影响；SDS-PAGE制备电泳纯化BCG HSP70并鉴定纯度，ELISA测定活性；流式细胞术检测负载抗原DC经BCGHSP 70活化后的表型；MTT法检测肝癌DC疫苗对自身淋巴细胞增殖的影响和混合淋巴细胞反应；流式细胞术检测肝癌细胞表面HLA-DR表达；MTT法检测肝癌DC疫苗对自身淋巴细胞的活化；原位杂交法检测肝癌DC疫苗活化后的淋巴细胞IFN-γmRNA表达；流式细胞术检测DC和肝癌DC疫苗活化后淋巴细胞表面Fas-L；MTT法分别检测肝癌DC疫苗活化的淋巴细胞和其培养上清对肝癌细胞的特异性杀伤和非特异性抑制作用；Cell-ELISA检测人肝癌细胞hAFP表达；MTT法检测负载AFP表位肽和凋亡肝癌细胞DC对自身淋巴细胞增殖的影响；ELISA法和MTT法分别测定活化后淋巴细胞培养上清中TNF和IL-12水平；肝癌细胞凋亡的诱导和检测；DC吞噬凋亡肝癌细胞后的电子显微镜观察；DC对肝癌细胞的生长抑制试验；人肝癌裸鼠皮下移植瘤动物模型的建立及其组织学和免疫组织化学鉴定；DC及肝癌特异性淋巴细胞预防和治疗人肝癌裸鼠皮下移植瘤；冻存和复苏后的肝癌特异性淋巴细胞预防和治疗人肝癌裸鼠皮下移植瘤。

Results The small regenerating fibers, central location of nucelus and basophilia endochylema were observed under HE stain both in the patients with DMD and PM. Anachromasis of antiNCAM monoclonal immunohistochemistry stain was found in the regenerating muscle fibers. Positive Ezrin expression was also detected in the regenerating fibers. However, this expression tapered gradually as the mature process of skeletal muscle. The expression of Ezrin was negative in the mature fibers.

结果 DMD、PM患者被检肌HE染色所见再生肌纤维直径较小、核位于中央、胞浆嗜碱性；NCAM染色再生肌纤维深染；再生肌纤维Ezrin呈阳性表达，伴随肌纤维成熟Ezrin表达逐渐减弱，成熟肌纤维无Ezrin表达；成肌细胞Ezrin呈阳性表达。

In this study, real-time PCR technology was used to dectect the expression of PRL mRNA and its receptor in the reproductive tissues,which is hypothalamus-pituitary-ovary-oviduct four important tissues. The aim was to compare the difference of PRL mRNA expression in the different state of ovulatory, broody and after broody, and to analyse the relation between expression and reproduction.

本试验利用real-time PCR技术检测雪山草鸡下丘脑、垂体、卵巢、输卵管四个繁殖轴组织中PRL基因及其受体的表达量，以确定处在生产、抱窝、醒抱三个不同状态母鸡PRL基因表达的差异，并分析基因表达量的差异与生产性能的相关性。

The expression of Pax-6 gene in the different stages was demonstrated by RT-PCR (Real - Time PCR):(1) The mRNA of Pax-6 gene expression was positive in Bufo raddei Strauch embryo at 16 stage; and the gene expression was also detected at 20 stage.

2荧光定量PCR结果显示在花背蟾蜍的晶状体再生的第7天Pax-6基因的表达量达到最高，表明此时期Pax-6基因在晶状体再生的过程中起到调控细胞增殖的作用。

All of these attested that there is CBF gene expression-regulated pathway and transcriptional cascade leading to the expression of cold-responsive genes under cold stress in Capsella bursa-pastoris, which ICE1 activate the expression of CBF by binding its promotor and CBF bind the DRE/CRT element in the promoter of the cold-regulated genes and activate transcription of them.

证明在荠菜中存在ICE1通过与CBF启动子部分相结合激活其表达，进而CBF又通过结合抗冻基因启动子部分带有的顺势式作用元件CRT/DRE从而促进抗冻基因表达，提高植物抗冻性的基因表达调控途径，及其它相关转录因子相互作用的级联机制。

The major results of this proposal are as follows:(1) ABA induces a rapid, substantial accumulation of apoplastic H2O2 in mesophyll and bundle sheath cells of maize leaves, and the accumulation of apoplastic H2O2 is involved in the induction of the chloroplastic and cytosolic antioxidant enzymes.(2) ABA-induced H2O2 production activates a 46 kDa MAPK, which in turn induces the expression of antioxidant genes and up-regulates the activities of antioxidant enzymes. The activation of MAPK also enhances H2O2 production, forming a positive amplification loop.(3) Water stress also induces the activation of a 46 kDa MAPK, which is dependent on the accumulation of ABA and H2O2 production induced by water stress and involved in the up-regulation of the expression and the activities of antioxidant enzymes.(4) ABA-induced H2O2 production mediates NO generation, which in turn activates a 46 kDa MAPK and results in the up-regulation in the expression and the activities of antioxidant enzymes in ABA signaling. NO-independent signaling is also involved in ABA- and H2O2-induced antioxidant defense.

本项目的主要研究结果如下：（1）ABA诱导的H2O2产生主要出现在叶肉细胞与维管束鞘细胞的质外体中，质外体H2O2的积累能够上调叶绿体与细胞溶质中抗氧化酶的活性；（2）ABA诱导的H2O2产生活化一个46kDa的MAPK，由此而导致编码抗氧化防护酶基因的表达与酶活性的上调；而MAPK的活化也能增强H2O2的产生，从而形成一个正的反馈调节环；（3）水分胁迫也能诱导一46kDa MAPK活化，这一MAPK活化依赖于水分胁迫诱导的ABA积累以及H2O2的产生，同时参与水分胁迫诱导的抗氧化防护基因的表达与抗氧化酶活性的上调；（4）ABA诱导一个依赖于H2O2的NO产生，NO活化一个46kDa的MAPK，从而导致抗氧化防护基因的表达以及抗氧化酶活性的上调；同时一个不依赖于NO的信号转导途径也存在于ABA诱导的抗氧化防护过程中。

The witness also told the jury at the Royal Courts of Justice in London that he saw a paparazzo fighting with a member of the public who was trying to stop him taking pictures in the minutes before the emergency services arrived.

他还告诉在伦敦皇家法庭的陪审团，在急救服务到来之前，他当时看见一个狗仔队正和一群阻止他拍照的人打架。

The entire N／C program on a tape is made up of an accumulation of these successive data blocks.

纸带上的整个数控程序由这些连续数据单元连接而成。