查询词典 operation expression

The larva-specific expression level of the function correlated to celluar components was the highest.The highest specific expression level of molecular function was appeared at neurula stage.A lot of functional specific expression about biological process was also appeared at neurula stage.7.The result showed that the pearson correlation coefficient was 0.896 compared between the sea urchin and Ciona savignyi.

进一步分析发现：在与细胞组件相关的功能特异性表达方面，幼体期的特异性表达量最高；在与分子功能活性相关的功能特异性表达中，神经胚时期的特异性表达量最高；而参与生物过程的功能特异性表达方面，神经胚时期的特异性表达量则最高。7不同物种间转录组分析显示尾索动物海鞘的转录组与棘皮动物海胆的最接近，其相关系数为0.896，而海胆与文昌鱼的相关系数只有0.682，结果表明在进化上文昌鱼可能与脊椎动物更接近。8运用Apache、php、MySQL技术搭建了Widows平台的文昌鱼EST分析数据库。

Construction and expression of the GFP expression vector is to study the expression of exogenous gene in NSC.

干细胞治疗与基因治疗结合，将是攻克很多难治性疾病的突破点。

It was found greater expression of NRP2-1 and NRP2-2 in pituitary, ovary, uterus and oviduct of ruttish Erhualian than corresponding tissues of ruttish Duroc, which suggested enhancing expression of NRP2 was favorable to increasing litter size. But the difference of NRP2 expression was not significant between two breeds, which suggested the key phase that NRP2 affected litter size was not oestrum probably, or NRP2 could enlarge cell signals and finally affect litter size ascribe that it was the receptor of nerve axon guidance factors.

NRP2-1和NRP2-2在发情期二花脸卵巢、子宫、输卵管中的表达量均大于杜洛克相应组织的表达量，推测NRP2表达水平高可能有利于提高产仔数，但两品种间表达差异不明显，推测NRP2影响产仔数的关键阶段可能不是猪的发情期；另外，NRP2 是神经轴突介导因子的受体，这些神经轴突转导的信号往往有级联放大的功能，虽然NRP2-1和NRP2-2在二花脸和杜洛克卵巢、子宫、输卵管中的表达差异不大，但二花脸有可能通过神经系统转导信号的级联放大最终引起一些效应激素或蛋白的急剧增加，从而提高排卵率或产仔数。

Scanning each character in the expression to separate and record operators, operational symbols and data types of operators, when expression being correct in syntax, recording interpreter executive order based on precedence of operator, a general expression interpreter is designed. The interpreter not only is used for numerical value and string computation, but also is easy to extend its functions, and when batch converting data, it could catch run error and continue to successive data.

2通过对公式的一次扫描，记录操作数、操作符号和操作数的赋值特性，并在语法正确的情况下，记录公式基于算符优先级的解释执行顺序，设计了通用公式解释器，它不仅可以进行数值运算、字符运算，而且可以对使用的函数库方便地进行扩充，在批量进行数据转换时，可以记录运行时错误，而不影响后续数据的执行。

Except is a set operator which returns a query expression that has all the elements of a query expression but not in another query expression.

除了是一家集商的查询表达式返回一个具有所有内容，包括在查询表达式，但在另一个查询表达式没有。

Producing PHAs,and then were orientationally inserted into a prokaryotic expression vector pBV220. By analyzing the bioactibity and function of their products, the biological function of phaA, phaB and phaC was confirmed. These have been done in our laboratory. To make better use of the three genes by plant bioreactor, two groups of different plant expression vectors that can be used to produce PHAs in different plants were constructed, and the transformation of PHA in Oxalis triangnlaris has been preliminarily reseached.The following is the main results:Three plant expression vectors pBI121-A,pBI121-B and ubquiting-C, which can be used to be transformed into Oxalis triangularis were respectively constructed by using the phaA, phaB and phaC.

菌株的亚克隆基因组片段中，分离出phaA、phaB和phaC三个基因片段，定向克隆至原核表达载体pBV220，通过对其生物活性与功能分析，确证了基因phaA、phaB和phaC的生物学功能；为了通过植物生物反应器对这三个基因进一步研究利用，本实验利用这三个基因构建了两组可用于不同植物生产PHA的真核表达载体，并对紫叶酢浆草进行PHA基因遗传转化的初步研究，获得主要结果如下：利用克隆的phaA、phaB、phaC三个基因分别构建了可用于转化紫叶酢浆草表达载体pBI121-A、pBI121-B、uBquiting-C；可用于玉米转化的种子特异性表达载体p7S-A、pBI121-S-B、uBquiting-C。

RESULTS A eukaryotic expression system for high expression humanmutantCD59 were successfully set up : The recombinant PALTER-MAX plasmid containing human mutantCD59 cDNA and PCDNA plasmid were co-transfected into CHO cell by cation lipoid mediating method ;and the cells were grown in F12 medium containing 400ug/ml G418 for 14 days, positive clones were grown in RPMI1640 medium to get stable expressing cell lines . Highly expressing clones were selected by flow cytometry ,and were named PALTER-CD59-CHO1PALTER-CD59-CHO2 . Flow cytometry indicated that expression rates of PALTER-CD59-CHO1 and PALTER-CD59-CHO2 were 53.7%and 54.5%. Further more, Stable highly expressing CHO cell lines were more detected by immunocytochemistry and immunofluorescence technology . PALTER-CD59 -CHO1 and PALTER-CD59-CHO2 were grown in RPMI1640 to get a large of cells . CD59 protein were obtained by spalling PALTER- CD59- CHO1 and PALTER - CD59 - CHO2 cells . Stable highly expressing cells were further validated by SDS-PAGE, immunoblot analysis and solid enzyme immunoassay . PALTER - CD59 - CHO1 and PALTER - CD59 - CHO2 were glycated in RPMI1640 of 50mM ribose for 72 hours .BCECF releasing test indicted that the releasing rate of PALTER-CD59-CHO1 or PALTER-CD59-CHO2 was less high than PALTER-CHO ,and the releasing rate of glycated PALTER-CD59 -CHOI or PALTER-CD59-CHO2 was higher than unglycated ones . PALTER -CD59-CHO1 and PALTER -CD59 -CHO2 were glycated in RPMI1640 of 50mM ribose for 72 hours .BCECF releasing test indicted that the releasing rate of PALTER - CD59 - CHOI or PALTER - CD59 - CHO2 was less high than PALTER-CHO ,and the releasing rate of glycated PALTER-CD59-CHO1 or PALTER - CD5 9-CHO2 was higher than unglycated ones .

结果 成功构建突变人CD59的真核细胞表达系统：运用阳离子脂质体介导法将含有突变人CD59的PALTER—MAX重组质粒与PCDNA共转染入CHO细胞：用含有400ug／mlG418的F12培养基培养14天，筛选出稳定阳性表达克隆，RPMI1640培养基扩增获得稳定表达细胞株，并用流式细胞术进一步筛选出高效表达细胞株分别命名为PALTER—CD59—CH01、PALTER—CD59—CH02，表达率分别为53.7％、54.5％；应用免疫组化方法、免疫荧光技术进一步鉴定阳性细胞株；RPMI1640培养基大量扩增PALTER—CD59—CH01、PALTER—CD59—CH02细胞株，裂解细胞得到CD59蛋白质；通过SDS—PAGE凝胶电泳技术、免疫印迹技术、固相酶联免疫吸附试验验证了这两中文摘要个阳性细胞株CO59蛋白的高效表达；50mM核糖培养72小时，获得突变人CD59糖化细胞株，BCECF染料释放试验结果显示，PALTER一CD59一CHOI、pALTER一CD59一CHOZ细胞较PALTER一CHO细胞染料释放率低，未糖化PALTER一CD59一CHOI、PALTER一CD59一CHOZ细胞比较糖化后细胞染料释放率低。

Cancellous bone from the lumbar proteomic expression analysis show that actin, keratin, enolase, ATP synthase, the expression of myosin may be related to ovarian lumbar cancellous bone after osteoporosis-related happened; Qiang Gu Bao Po on the lumbar spine osteoporosis intervention effect may be related to reduced carbonic anhydrase, actin, crystal protein, 3- phosphoglycerate dehydrogenase, serum albumin, ATP synthase, myosin, the enolase expression.

从腰椎松质骨蛋白质组表达分析可知，肌动蛋白、角蛋白、烯醇化酶、ATP合酶、肌球蛋白的表达增强可能与去卵巢后腰椎松质骨骨质疏松的发生相关；强骨宝对腰椎骨质疏松的干预效应可能与下调碳酸酐酶、肌动蛋白、αB-晶体蛋白、3-磷酸甘油醛脱氢酶、血清白蛋白、ATP合酶、肌球蛋白、烯醇化酶的表达有关。

RESULTS: Both resting B cells and T cells did not express PD-L1 on their surface, however PD-L1 expression was significantly up-regulated on the surface of the activated B cells after 6 h stimulation with LPS or pokeweed mitogen, and the percentages of B cells that expressed PD-L1 reached a plateau at 24 h, which were (46.26±10.71)% with LPS and (43.67±6.14)% with PWM stimulation, respectively. No markedly change of PD-L1 expression on the surface of the activated T cells after stimulation with LPS was observed, but upregulation of PD-L1 expression was observed when stimulation with PWM.

结果： 静息B细胞和T细胞表面不表达PD-L1，LPS和PWM刺激6 h后表达PD-L1的B细胞百分率均显著高于静息B细胞，24 h达到最高，分别为(46.26±10.71)%和(43.67±6.14)%，之后随时间延长而降低；表达PD-L1的T细胞百分率在LPS刺激下无显著变化，但PWM刺激6 h后百分率明显高于静息条件，24 h达到最高，为(25.42±9.23)%，之后下降。

Transgenic tobacco plants were obtained through screening with kanamycin. The transgenic tobacco plants could delay TMV infection for about 25 days compared with non-transgenic tobacco plants. Pokeweed antiviral protein Ⅱ is expressed with high level in summer leaves. The expression of PAPⅡ is regulated by season. The total RNA was extracted from pokeweed (Phytolacca americana L.) leaves in summer using the method of TRIzol and used as template to amplify the PAPⅡ gene by RT-PCR and then the gene was cloned into E. coli expression vector and secreted expression pPIC9K vector. The two vectors with PAPⅡ gene were then transferred into E. coli strain BL21 (DE3)-plysS and Pachia pastoris GS115 strain respectively. The specific protein was produced induced by IPTG and methanol.

由于美洲商陆抗病毒蛋白Ⅱ是一个受季节调控表达的蛋白，本实验以美洲商陆夏季叶片为材料，通过对其总RNA的提取、反转录、并用PAPⅡ的特异引物进行PCR扩增，对PAPⅡ进行了基因克隆、序列分析，结果扩增出来的PAPⅡ基因与已经报道的序列同源性是99.9％，然后将该基因构建到原核、真核表达载体上，分别转化了大肠杆菌和毕赤酵母并对它们进行了诱导表达，在两个表达系统中均获得了有活性的PAPⅡ表达蛋白，体外生物测定表明表达的蛋白均具有抑制病毒的活性，PAPⅡ基因在酵母中还没有表达的报道，这为获得具活性PAPⅡ蛋白提供了一种简便可行的方法。

Putt your way through 36 fun-filled holes of minigolf on 3D designed courses with elevated greens, bunkers, bridges and water hazards, among other crazy obstacles.

您的推杆方式，通过36个有趣的填孔迷你的三维设计的课程，以提升绿党，掩体，桥梁和水的危害，除其他疯狂的障碍。

Some participles can be used either as attributes or as predicatives.

有些分词既可当定语用，也可当表语用。