查询词典 oocytes

In order to find an effective nuclear transfer program, this thesis investigated three factors affecting the efficiency of somatic nuclear transfer on goats: the in vivo mature time of oocytes, the component of operation liquid for oocyctes enucleating and the parthenoactivation mode of oocytes.

本实验通过改变以下试验条件：卵母细胞体外成熟时间的不同、卵母细胞去核时操作液成分的不同，来研究其对山羊核移植效率的影响，希冀寻找一个有效的核移植方案。

Enucleating the nuclei of recipient oocytes accurately is one of the key techniques in nuclear transfer research. Enucleation of mouse MⅡoocytes can be performed under an inverted microscope equipped with Normarski or Hoffmann optics.

准确去除受体卵母细胞的细胞核是核移植研究的关键技术之一，在装备有Normarski 或Hoffmann 光学镜头的倒置显微镜下可以准确去除小鼠MⅡ期卵母细胞的细胞核。

Cell embryos were cultured in vitro, then viability was observed. The results showed the fertilizing rate in vitro of the group for GV oocytes with cumulus cells after maturating which were conserved at 10℃ for 24 h, 15℃ for 14 h, 20℃ for 8 h and 25℃ for 4 h was 14%, 9%, 10% and 10% respectively, and with the advance of storage temperature and extension of storage time, the rate of 2-cell after maturating-fertilizating of the GV oocytes decreased significantly.

其结果，在10℃下保存24h、15℃下保存14h、20℃下保存8h和25℃下保存4h后，其体内附有卵丘细胞的GV卵的体外成熟－体外受精后的2细胞率分别为14%、9%、10%和10%，随着保存温度的提高和保存时间的延长，带有颗粒细胞GV期卵的比率明显降低，同时其GV期卵经体外成熟及体外受精后的2细胞率明显降低。

This study was carried out to investigate whether the GV oocytes from carcasses could be utilized. Carcasses were preserved at 10℃, 15℃, 20℃ and 25℃ for 8 hours, 14 hours, 24 hours, or 48 hours after mice were killed and GV occytes were collected. After maturating in vitro, these oocytes were fertilized in vitro with the general procedure in advance.

将ICR系雌性小鼠处死并在10℃、15℃、20℃和25℃下依次保存8、14、24和48h后，采集其体内的卵巢GV期卵，采用常规方法进行体外成熟和体外受精，获得的2细胞期胚经体外培养或胚胎移植观察其发育能力。

Rates of fertilization and morulae/blastocysts in oocytes with fully expanded cumulus were significantly higher than those in oocytes with poorly expanded cumulus.

扩展良好的山羊卵巢卵母细胞的受精率和胚胎发育能力显著高于卵丘扩展差的卵母细胞。

Strong expression of nanosl mRNA can be observed in oogonia and stage Ⅰ、Ⅱ oocytes but the signal became weaker in later stage oocytes.

在斑马鱼精子发生中，nanoslmRNA可在精原细胞和初级精母细胞中检测到。

Artificially induced a single 〓elevation could effectively drive the activation, in recently ovulated oocytes (12—13 h after hCG) however, a single Ca〓 increase was futile in initiation of activation and only repetitive 〓 rises were avalable, suggesting that the activation of recently ovulated oocytes requires oscillatory Ca〓 signals as occuring at fertilization.

只有诱导卵内Ca〓多次升高才能使之激活。提示：刚排出不久的卵激活象受精一样需要胞质Ca〓波动信号。

Results The expression of Foxo3a, Bim, FasL in oocyte nests and primordial follicles were detected in the nuclei and cytoplasms of some oocytes. The patterns of Bim and FasL positive rates were consistent with that of Foxo3a and TUNEL positive oocytes rates, which reached the peak in the ovaries of 2-day-old rats.

结果 免疫组化显示Bim和FasL都在新生大鼠部分卵母细胞巢和原始卵泡的卵母细胞核内和胞浆高表达，且阳性率曲线都在2d达到高峰，随后逐渐降低，与TUNEL和FoXo3a的阳性率变化曲线一致。

This thesis compared the effect of Uracil, ITS, and ATP on the maturation of bovine oocytes and further development, observed the microstructure changes of oocytes and parthenogenetic embryos among different groups, and granule cell apoptosis among different groups.

本文对比了添加尿嘧啶和三价因子等试剂对牛卵母细胞成熟及后期发育的影响、观察了不同组之间成熟牛卵母细胞和孤雌激活胚胎显微结构变化和不同组之间颗粒细胞凋亡情况。

The meiosis of oocytes differ from mitoses of general cells. Especially in most mammalian species, oocytes are formed during fetal life, but they are arrested at the prophase stage of the first meiotic division until around the time of ovulation.

卵母细胞的成熟分裂有别于一般细胞的有丝分裂，尤其是哺乳动物，在胎儿期或出生后不久，初级卵母细胞就陆续进入第一次成熟分裂前期阶段，但长时间的&休止&于此期，直到性成熟时在促性腺激素等因素的作用下，第一次成熟分裂才重新启动。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。