查询词典 oocytes

In order to investigate the biophysical characteristics of porcine oocytes, mechanics property of the zona pellucida of porcine oocytes was studied.

［目的］为了探讨猪卵母细胞的生物物理特性，该文研究了猪卵母细胞透明带的力学性质。

Results On day 15th and 30th,compared with control group,the experimental group presented more primordial follicle,primary oocytes,mature oocytes than control group,and showed less accrementition and hyalinization in ovarian vessel.

结果实验组与对照组相比较，在骨髓移植第15、30天，对照组成熟卵泡数量减少，卵巢血管增生，有纤维化；实验组可见原始卵泡，初级卵泡，次级卵泡，成熟卵泡，黄体数目较多。

The rate of Flow cytometry to analyse retinoic acid haploid is 19.3%.The sperm-like cells achieved could activate bovine oocytes by sperm injection, and the development rate of blastosphere was 20.36%. Conclusions SSCs of newly -born mice can be induced and differentiated into sperm-like cells and are also capable of activating oocytes.

本实验通过体外诱导新生小鼠SSCs分化为精子样细胞，探讨不同诱导方法的诱导效率；采用显微注射法将精子样细胞注射到卵母细胞和去核卵母细胞，探讨其体外重构胚的发育能力，为SSCs体外分化研究打下基础。

Oocytes were cultured in Oocytes Maturation medium with EGF and IGF-1 alone or combinedly at different concentration.

研究了在培养液中添加不同浓度的EGF﹑IGF-1 以及EGF 联合IGF-1 对山羊有腔卵母细胞体外成熟的影响。

After that , the comparison of the fertilizable competences of ICSI and IVF on cryopreservated oocytes was studied in this article , in order to establish an optimized method to improve the fertilizable competences of oocytes up to the level of embryos .

和胞浆内单精子注射两种方法对玻璃化冷冻卵母细胞的受精能力进行了比较研究，旨在建立能够进一步提高牛冷冻卵母细胞受精能力的改进方案，使卵母细胞冷冻技术上升到与胚胎冷冻同样成熟的水平。

Ovine immatured oocytes, matured oocytes and embryos from 2-cell stage to blastocyst produced in vitro were recovered, and RT-PCR was used to semi-quantify mRNA expression. Proteins of Cx43 and Cx45 were detected by using a combination of immunocytochemistry and Laser Scanning Confocal Microscope.

收集绵羊未成熟和成熟卵母细胞以及体外受精早期胚胎各发育阶段的卵裂球细胞，通过RT-PCR半定量检测Cx43和Cx45基因的mRNA表达量；通过免疫组织化学方法结合激光扫描共聚焦显微镜检测Cx43和Cx45蛋白在绵羊体外受精的早期胚胎发育过程中的表达情况及表达区域。

After 44 h IVM, MOS level in the immatured ooeytes was higher than matured oocytes, and there was less MOS in nucleus than in the cytoplasm at 6 h after matured oocytes were activated; RNAi was performed before IVM, and all the three siRNA applied could successfully knockdown the level of c-mos mRNA, which were 0.08 ± 0.03, 0.11 ± 0.06 and 0.20± 0.06 times of the control group respectively, and the quantity of MOS was obvious decreased compared with the control group at the same stage.

本实验通过RT- PCR、免疫荧光激光共聚焦检测方法检测了猪卵母细胞在体外成熟培养过程中c-mos基因在转录水平、翻译水平上的表达以及蛋白的分布，并应用注射小干扰RNA方法对其进行了RNA干扰研究。

Hormone level in maturation medium affected cumulus expansion of COC, but cumulus expansion became less dependent on hormone level with time of culture. Porcine immatured oocytes, granulosa cells from small antral follicles and cumulus cells of immatured oocytes secreted cumulus-expansion enabling factors. Granulosa cells from 3-6mm follicles did not produce CEEF, but there was some CEEF in the follicular fluid of this sized follicles.

激素水平对卵丘扩展有影响，但随培养时间的延长，对激素的要求有逐步减少的趋势；猪卵丘扩展不依赖于卵母细胞，卵母细胞核成熟也与卵丘扩展无关；GV期卵母细胞、小腔卵泡内颗粒细胞和成熟卵的卵丘细胞都有较高的分泌CEEF能力；3-6mm卵泡的壁颗粒细胞不能分泌CEEF，但3—6mm卵泡的卵泡液中有CEEF。

Confocal laser scanning microscopy used to localize the hsp70 protein in oocytes and embryos revealed that the distribution of hsp70 in the ooplasm of immature and mature oocytes is unaffected by exposure to decreased or elevated temperatures and that this protein was closely associated with the meiotic spindle indicating its possible role in stabilizing this structure, hi eight-cell embryos derived under control conditions, hsp70 was evenly distributed in the cytoplasm but appeared as aggregates in some embryos exposed to elevated temperature.

此蛋白均匀地分布在两种细胞的胞浆中。此形态特点与减数分裂期纺缍体有关，由此表明hsp70在中期Ⅱ期，具有稳定这种结构的作用。在对照组8细胞胚中，hsp70均匀地分布在胞浆中，而在处理组一些胚胎内，hsp70则发生凝集。

Staining analysis for porcine oocytes was conducted by using 0.1% Orcein solution in order to investigate the role of cumulus cells in nuclear maturation of porcine oocytes.

用0.1%地衣红对猪卵母细胞进行染色检查，分析不同层数卵丘细胞对卵母细胞成熟的影响。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。