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GV oocytes with normal shape were not obtained in the group which was stored at 20℃ for 24 h and at 25℃ for 14 h; A blastula rates (64%) of all groups in average was gotten in culturing 2-cell in vitro, and there is no significant difference in temperature and time of storage. After transplantating some of the 2-cell embryos which were obtained from GV oocytes stored at 15'C for 8 h, normal infant mice could be obtained.

在20℃下保存24h和25℃下保存14h时，难以获得形态正常的GV期卵；体外受精获得的2细胞期胚经体外培养，总体上有64%的胚胎发育至扩张囊胚，未见有保存温度和保存时间的显著影响，且利用在15℃保存8h后的GV卵获得2细胞期胚的移植获得正常新生小鼠。

In addition, using RT-PCR to detect expression of centrin homogene during Urechis unicinctus spermatid deferentiation and early embryonic development. The result revealed that (1) expression of HsCEN1 homogene is present during spermatid defferentiation including spermtid and sperm, but not in adult tissues including body wall tissue,intestinal mucous memberane cells and cell in coelom succus;(2) expression of HsCEN1 homogene is not present in unfertilized oocytes but present in that of released polar bodies after fertilization and parthenogenesis oocytes treated with A23187;(3) expression of HsCEN1 homogene is present during early embryonic development including two-cell stage,multicell stage,gastrula to trochophora.

我们还用RT-PCR方法检测了Centrin同源基因在单环刺螠精子分化过程和早期胚胎发育过程的表达，结果显示：在单环刺螠精子发生过程中的精细胞、精子团、精子中都有表达，而在成体的体壁组织、消化道组织、体腔球中则没有表达；在未受精卵中没有表达，而在受精后释放极体的受精卵和经A23187孤雌激活的卵中则有表达；在单环刺螠的早期发育过程——二细胞胚胎、多细胞胚胎、原肠胚、早期担轮幼虫——中都有表达。

Although the ability to reprogramming of Kun Ming white mouse oocytes cytoplast is not effectively, reprogramming ability has improved when cystoplasts from oocytes and embryos work together.

昆明白小鼠卵母细胞胞质对体细胞核的重新编程能力较弱，而当其与正常受精卵的胞质共同作用时可较好地改善对体细胞的重新编程能力。

Microinjection was used to inject the sperm-like cells into oocytes and enucleate oocytes to observe the development situation of zygotes. Results The proportions of sperm-like cells induced by the three methods were 5.9%,1.6%,0.35% respectively.

但在体外研究SSCs诱导精子的很少，某些雄性性原细胞可以直接分化成精原细胞［6］，这提示具有一少部分雄性性原细胞维持在干细胞状态。

The effective mechanism may have relation to the integral regulating function of Chinese herbal medicine which can promote the upgrowth of oocytes,adjust immune function, improve the level of reproductive endocrine, quality of oocytes and endometrial receptivity.

其作用机理与促卵细胞发育、改善生殖内分泌环境、调整免疫功能及提高子宫内膜容受性的中药整体调节作用有关。

The results indicated that spindles, chromosomes and microfilaments of porcine oocytes at different stages were nonreversible damaged obviously followed exposion to freezing medium or vitrification, which may be the main reason affecting the maturation, fertilization and development of oocytes.

本研究结果表明，猪GV期和MⅡ期卵母细胞经冷冻保护剂处理或玻璃化冷冻保存后，都明显造成了纺锤体、染色体和微丝不可逆的损伤，可能是影响卵母细胞成熟、受精与发育的重要原因。

These results indicate that,(1) appropriate concentration of CHX can enhance the activation efficiency of oocytes;(2) CB can increase the two pronuclei formation rate of oocytes by inhibiting the extrusion of second polar body.

这些结果说明：(1)适当浓度的CHX能提高卵母细胞激活效果；(2)CB能抑制卵母细胞激活后第二极体排出，从而提高原核的形成率。

These results indicate that CHX at an appropriate concentration can improve the parthenogenetic development of buffalo oocytes, CB at an appropriate concentration may inhibit the extrusion of the first polar body and increase the proportion of activated oocytes with two pronuclei.

结果表明，适当浓度的CHX能提高卵母细胞激活效果，适当浓度的CB可抑制卵母细胞第二极体的排出，提高孤雌激活后双原核的比例。

Addition of 3μg/ml CB to CM containing 0.625μg/ml CHX increased the proportion of oocytes with two pronuclei (38. 9% vs 15. 1%, P＜0.05) and developed into morulae/blastocysts (40.7% vs 26. 8%, P＜0.05), but did not affect the percentage of oocytes cleaved and total pronucleus rate.

如果在含有0.625μg/ml CHX中添加3μg/ml CB，可明显提高双原核的形成率（38.9％vs 15.1％，P＜0.05）和桑椹胚/囊胚发育率（40.7％vs 26.8％，P＜0.05），但对其激活分裂率和总原核形成率无明显影响（P＞0.05）。

The localization of 〓 changed during the process of rat oocytes maturation. In GV stage oocytes,〓 was concentrated in GV but it concentrated around chromosomes from metaphase Ⅰ to telophase Ⅰ.

〓的在大鼠卵母细胞内的定位随成熟分裂的过程而变化：在GV期生发泡内核仁周围有特异分布，在成熟分裂的中期至末期〓在染色体周围区域有特异分布。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。