查询词典 oocytes

Of reconstructed oocytes with 2-cell blastomere and 0.7% of reconstructed oocytes with ES cell developed in vitro to blastocyst.

结果表明不同分化程度的细胞核移植后，重新编程的难易程度不同，分化程度越高则重新编程越难。

In conclusion, these results suggested that (1) the ageing-associated decline in fertility of female KM mouse was due to a decrease in both quantity and quality of oocytes;(2) the ooplasm from young mice did not correct the meiotic errors of aged mice, and the ooplasm from aged mice did not induce abnormal segregation of meiotic chromosome of young mice, indicating that meiotic anomalies found in the oocytes of aged mice might be related to nucleus or chromosomes and relevant factors;(3) a critical nucleocytoplasmic ratio was essential for normal maturation and segregation of meiotic chromosomes of oocytes and development to 2-cell embryos.

本研究结果表明：（1）昆明白小鼠与衰老相关的生育力下降是其卵母细胞数量减少和质量下降的综合结果；（2）年轻小鼠卵母细胞细胞质不能纠正老龄小鼠GV的减数分裂错误，老龄小鼠卵母细胞细胞质也不诱导年轻小鼠的GV发生减数分裂错误，老龄小鼠卵母细胞减数分裂异常很可能与细胞核或染色体及其相关因素有关；（3）关键的核质比对卵母细胞正常的成熟、减数分裂染色体分离及2-细胞期胚的发育是绝对必需的。

Cumulus-enclosed oocytes from follicles in prepubertal gilt ovaries from alocal abattoir were cultured in modified TCM199 with and without 10 IU/ml PMSGand hCG for 22 hours respectively.After fixation and dying in 1% acid orcein,itshowed that 77.6% oocytes reached MII stage.Maturated oocytes were denudedwith 1mg/ml hyaluronidase,and fertilized with fresh or thawed sperms in modifiedTBM for 6 hours,then cultured in NCSU23 for 14 hours.The fertilization rates were87.5% for fresh sperms,64.1% for thawed sperms.

抽取从屠宰场获取的猪卵巢中的卵母细胞，在加入和不加hCG和PMSG的mTCMl99培养液中分别培养22小时，地衣红染色发现77.6％的卵母细胞达到MII期；去卵丘细胞后在mTBM中用鲜精或冻精解冻后受精6小时，在NCSU23中培养14小时后固定染色发现受精率分别为87.5％和64.1％；电激活后在NCSU23中培养20小时后固定染色发现原核形成率为94.1％。

Most of EGFR in unmatured and matured oocytes was in variation, and there was obviously more expression of variation in matured oocytes than that in unmatured oocytes.

未成熟和成熟的卵母细胞中主要存在Ⅷ突变型，而野生型表达相对较少，成熟后卵母细胞中的Ⅷ突变型蛋白明显增加。

The results were as follows: nanos 1 mRNA uniformly dispersed throughout the cytoplasm of oocytes at all stages. Strong expression of nanos1 mRNA was observed in oogonia and stageⅠ、Ⅱ oocytes, but the signal became weaker in later stage oocytes.

结果表明：在斑马鱼卵子发生中，nanos1 mRNA均匀分布于卵原细胞和各时期卵母细胞的胞质中；在卵原细胞和Ⅰ、Ⅱ期卵母细胞中，nanos1 mRNA的杂交信号十分强烈，而较晚期卵母细胞中信号明显减弱。

Resembled to control oocytes, MAPK was inactivated after cytoplasts activation. However, after activation the cytoplasts showed fragmentation while pronuclei were formed in the control oocytes. This indicated that nuclei play important role in maintainence of oocytes normal morphology.

成熟胞质的活化与卵母细胞相似，在活化后MAPK被迅速灭活，只是胞质在活化处理后均发生碎裂，而卵母细胞活化后形成原核，这表明核在细胞形态维持中起重要作用。

B:Oocytes and early embryos were permeated in 0.5%Triton X-100 for 10 minutes,then fixed in 4% paraformaldehyde/PBS.Method C:Oocytes and early embryos were fixed in 1%PFA while permeated in 0.2%Triton X-100 at the same time for 1 hour.Method D: Oocytes and early embryos were fixed in precooled methanol(-20℃) for 15 min,then permeated in 0.5%Triton X-100 for 10 minutes.

方案A：先用4%多聚甲醛固定1h，再用0.5%Triton X-100穿透10min；方案B：先用0.5%Triton X-100穿透10min，再用4%PFA固定1h；方案C：用1%PFA+0.2%Triton X-100同时固定与穿透1h；方案D：用-20℃预冷甲醇固定15min，再用0.5%Triton X-100孵育10min。

It is necessary to develop a protein-free media for oocytes maturation that can prevent zona hardening. In this study, immature macaque oocytes were cultured in modified CMRL media alone or CMRL with either bovine calf serum or PVA for 24 hours. Also, in vivo matured oocytes were cultured in CMRL supplemented with PVA or serum for approximately 6 hours.

我们在本研究中，将非成熟的猕猴卵母细胞分别在改良的CMRL，或在含有胎牛血清或聚乙烯醇的改良CMRL培养基中培养24小时；同时，将体内成熟的卵母细胞分别在含有PVA或者胎牛血清的CMRL中培养6小时，然后进行体外授精，比较各组间精子穿透率或受精，以及胚胎发育情况。

The results showed that chromium trichloride could inhibit the extruding of the first polar body and affect the quality and the viability of mouse oocyte and reduce the rate of IVF and the number of oocytes of superovulation, inhibit the germinal vesicle breakdown of mouse oocytes in vitro culture, but little impact on GVBD of mouse oocytes in vivo .

结果表明，三氯化铬可以抑制卵母细胞第一极体的释放，降低小鼠超排卵数和卵母细胞的存活率和体外受精率。

The cumulus cells of OCC were cut off and dispersed by 1 mL syringe. The cumulus cells were co-cultured with the immature oocytes retrieved from the COH cycles after they adherent to the bottom of the dish. The immature oocytes were experienced IVM procedures in different culture media. They were divided into 3 groups(the oocytes at germinal vesicle stage from one woman were allotted to the same group randomly). Group 1: basic culture medium+ human follicular fluid; Group 2: solution A+ cumulus cells; Group 3: solution A+ OCC+ follicle stimulating hormone + epidermal growth factor. Then, the maturation rate, fertilization rate and formation rate of available embryo were observed.

在控制性促排卵周期有未成熟卵母细胞时，将同周期成熟卵丘复合体切出部分卵丘细胞，用1 mL注射器抽打分散细胞，贴壁培养。113个治疗周期中，298枚生发泡期卵母细胞经3种不同培养液体外成熟培养(同一病人的生发泡期卵被随机分到某同一组中)：第1组28个周期中73枚：基础培养液+卵泡液；第2组40个周期中115枚：A液+分散贴壁的卵丘细胞；第3组45个周期中110枚：A液+分散贴壁的卵丘细胞+促卵泡生成激素+表皮生长因子。

But we don't care about Battlegrounds.

但我们并不在乎沙场中的显露。

Ah! don't mention it, the butcher's shop is a horror.

啊！不用提了。提到肉，真是糟透了。