查询词典 oocytes

In one group oocytes were cultured for 3h and in the other one for 6h. Incidence of germinal vesicle breakdown was observed following the culture with BL-Ⅰ.

BL-Ⅰ培养液培养结束时，观察卵母细胞生发泡破裂的发生率，再用不含BL-Ⅰ的培养液继续培养，进一步观察其成熟动力学的变化，即卵母细胞GVBD过程和第一极体形成过程。

Methods: Oocytes in the GV stage were separated from ovary by squeezing method. In mouse germinal vesicle GV stage, the expression of ATP8 gene in the mitochondria in the single oocyte was detected by RT-PCR, in which, cDNA was synthesized with two methods: one was the single GV-stage oocyte directly to be placed RT, the other was to perform RT after eliminating mtDNA and nucleus DNA with the EeoR Ⅰ enzyme and Dnase. And the product of RT-PCR was cloned and sequenced.

应用挤压法从卵巢中分离获得生发泡期(germinal vesicle， GV)卵母细胞；用RT-PCR检测GV期单个卵母细胞中ATP8基因的表达：其中cDNA的合成分两种方法进行：一是将GV期单个卵母细胞直接进行RT合成cDNA，二是先用DNA酶加EcoR Ⅰ酶祛除mtDNA和核DNA后再进行RT；回收产物构建克隆质粒并测序。

In this study, we investigate the cryobiology character of rabbit and human oocytes inorder to find a better freezing method, which can improve the survival rate and the following embryo development. We hope this study can provide some laboratory proofs for the future use of this technique.

本研究旨在探讨卵母细胞的冷冻生物学特性，寻找更好的冷冻方法进一步提高存活率，减少解冻后卵母细胞及其所形成胚胎的损伤，建立可行的、冷冻后存活率及发育率较高的方案，为此技术最终应用于临床提供一定的实验室依据。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。