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Sequential culture system is more suitable for the development of cultured human oocytes and embryos.

结论采用适合的培养液进行序贯培养,更适合于卵子及胚胎的体外发育,方法安全可行。

The ovarian development before the first sexually mature and oocytes were divided into five stages. It needed about 3 years for the Pref.

卵巢在第一次性成熟过程中的发育分为5期,其生殖细胞相应分为5个时相。

Despite SPH treatment was an effective and indispensable method to hasten artificially sexual maturation, and the size-frequency distribution indicated that the most developed oocytes of those were growing from the vitellogenic stage to the final maturational phase.

观察卵径频度分布显示,虽然在人工诱导性腺成熟过程中,鲑鱼脑下垂体研磨液是非常必要之激素,可有效促进日本鳗卵细胞从卵黄蓄积阶段发育至卵细胞最后成熟阶段。

The objective of the present study was to investigate the effect of Follicular stimulating hormone and Luteinizing hormone on in vitro maturation of porcine oocytes,and to find the optimal doses of them.

在成熟液中添加LH浓度分别为1,5,10 IU/mL时,与没有添加FSH与LH的对照组(15.9%)相比,成熟率有显著提高,成熟率分别为49.1%,30.0%,36.3%。

Cell-cycle synchronization between the donor cell and recipient oocyte determines the embryo development in nuclear transfer. In the present study, we microinjected primary spermatocyte into the perivetelline space of oocyte. 37% pairs fused after electric stimuli and the resulting oocytes were culture for 2 h in MEM with or without CB.

在本研究中,我们将小鼠的初级精母细胞显微注入MI卵母细胞的透明带下,经直流电脉冲作用后有37%的初级精母细胞融入卵母细胞,然后将融合的卵母细胞分成两组在MEM和含有CB的MEM培养液中分别培养,2小时后将在CB培养液中培养的卵母细胞转入正常培养液中。

The results showed that after 2 h of culture in MEM the chromosomes of oocyte were seperated to form telophase I while a small spindle was observed around chromosomes of primary spermatocyte. However, two clear spindles were observed in the oocytes cultured in CB containing MEM. After further culture, the chromosomes of both primary spermatocyte and oocyte intermingled and formed one large spindle.

在无CB的培养液中培养的卵母细胞培养2小时后,卵母细胞已经进入第一次减数分裂的后期,染色体开始被拉向两极,而精母细胞的MI纺锤体才刚刚形成,虽然继续培养两者染色体可以合二为一并形成一个纺锤体,但是有些染色体发生滞后;当卵母细胞在含有CB的培养液中培养2小时后,在卵母细胞内形成两个相似大小的MI纺锤体,进一步培养形成一个大的纺锤体,染色体正常。

Previous study has shown that roscovitine did not inhibit germinal vesicle breakdown of goat oocytes until the concentration increased to a high dose.

之前的研究证明,高浓度的ROS能抑制山羊卵母细胞发生GVBD,但长时间高浓度抑制损伤卵母细胞的发育能力。

This study compared outcomes of in vitro maturation and in vitro fertilization intracytoplasmic sperm injection cycles after IVM of immature germinal vesicle oocytes.

研究对比体外受精-单精子卵胞浆内注射周期所获生殖泡期不成熟卵母细胞以及体外成熟周期所获GV期卵母细胞体外培养成熟后的胚胎发育情况。

LH plaies a role to delay the occurrence of germinal vesicle, prolong the maturation duration of cytoplasm, enhance the nuclear and cytoplasm synchronization of ovine oocytes matured in vitro, and finally improve the potentiality of their in vitro development.

LH可能通过延迟生发泡破裂和促进胞质成熟的方式,使绵羊卵母细胞的核质成熟趋于同步,有助于绵羊卵母细胞的体外成熟以及受精后的进一步发育。

Germinal vesicle transfer is a technique developed in recent years, which may be a valuable research procedure to study cell cycle regulation and characterize the cytoplasmic-nuclear interplay during maturation and fertilization of oocytes.

通过GV移植可以研究细胞周期调控、成熟及受精时细胞核与细胞质之间的相互作用。

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