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Enucleated rabbit oocytes would be used as recipients were activated by electric stimulus before nuclear transfer.

兔去核卵母细胞先经单个电脉冲进行活化刺激,然后进行核移植。

A new method for enucleating MⅡ nucleus in mouse and rat was induced; Living births were obtained from IVF of mouse oocytes reconstructed by transfer of metaphase Ⅱ chromosomes. For the interspecies nuclear transfer between mouse and rabbit, the reconstructed embryos could develop into blasocyst in vitro.

在不同品系小鼠卵母细胞核质交换移植中将电融合的重构卵进行IVF,经胚胎移植后顺利产仔;在异种核移植方面,小鼠核与兔卵母细胞的异种重构胚在体外培养条件下发育至囊胚阶段。

Under the transmission election microscope, we had observed that the state of oocytes arid the cumulus cells around them was tightness, and there were plenty of long and thin microvilli playing the role of connection in the enwrapping. The shape of cumulus cells nucleus was ovate, and the longer and shorter diameters of the cumulus cells were 4.250±0.042μm and 2.750±0.07 μm respectively. The zona pellucida was well proportioned and tightly linking with oocyte. There were great deals of multi-crista mitochondria, lipid droplet and vacuolus. The mitochondria longer and shorter diameters were 0.600±0.106μm and 0.490±0.117μm. Granular nucleoplasma distributed very well in the cell nucleus.

电镜下,卵母细胞外的卵丘细胞紧紧包裹卵母细胞,且与卵母细胞之间有大量细长的微绒毛相联系;卵丘细胞核呈卵圆形,其长径为4250±0.042μm,短径为2.750±0.071μm;透明带均匀,与卵母细胞结合紧密;在皮质区集中分布大量的多峰型线粗体、脂滴和空泡,线拉体长径为0.600±0.106μm,短径为0.490±0.117μm;细胞核中颗粒状的核质分布非常均匀。

Among the three kinds of activation methods, electrical activation was the best with (67.75±1.03)% oocytes cleavage rate and (13.52±2.05)% morulae rate. The best parameters for electrical activation was 130 V/mm and 80 μs.

电激活比乙醇激活、A23187激活有更高的卵裂率(67.75±1.03)%和桑椹胚率(13.52±2.05)%,且差异显著(P.05),最佳激活参数为130V/mm、80μs、一次脉冲激活。

The results show the Boer goat oocyte and pre-implantation embryo are telomerase positive. According to the densitometry of bands under the CCD imagine system, the total telomere repeats product generated of oocytes, 4-cell, 8-cell, morulae and blastocyst are 25.348, 273.832, 56.117, 251.118, 519.46, respectively.

结果表明波尔山羊卵母细胞和早期胚胎都是端粒酶阳性;依据电泳条带在凝胶成像系统下的光密度计算相对总产品产量定量比较发现,卵母细胞最低为25.348,4-细胞为273.832,至8-细胞又降低到56.117,随后快速升高,桑椹胚为251.111,囊胚端粒酶活性最高为519.46。

Sex selection by manipulating the conditions of sperm-oocytes fertilization would be one of the most practicable and widely used methods.

控制受精环境即可控制性别,这种方法不需昂贵的仪器设备或者药品,易实践,可普遍应用,其经济效益和社会效益难以估量。

The aim of this study was designed to explore the possibility oftransferring bovine embryo blastomeres into buffalo nucleated oocytes.

本实验探讨了将体外培养成熟的水牛卵母细胞去核后,作为体外受精生产的黄牛胚胎细胞核移植的受体进行种间核移植的可能性。

In the female, ER was distributed in the nucleus of oogonia cytoplasm and membrane of early primary oocytes.

因此,可以认为雌性激素ER结合唤醒了&休眠&的卵原细胞和精原细胞,细胞核发生一系列成熟分裂期的变化,从而启动了卵子和精子发生的开始。

The results indicated that the receptors of estrogen, androgen and progestogen existed in oogonia and oocytes of different developmental stages.

结果表明,不同发育时期的卵原细胞和卵母细胞中都存在雌激素、雄激素和孕激素受体。

Mid-gestational fetal ovaries contain a complete spectrum of oogonia and oocytes in prophase.

女性的生殖功能在胎儿期已奠定了基础。其一生中全部卵细胞都在胎儿期增殖形成。

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