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oocytes相关的网络例句

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Methods: Human mature oocytes were cryopreserved using cryoleaf. Resuscitated oocytes were administered by intracytoplasmic sperm injection, embryo transplantation or cultured to blastulation, observing the fertilization and developmental capacity of oocytes.

运用cryoleaf进行人类成熟卵母细胞玻璃化冷冻保存,复苏后行胞浆内单精子注射-胚胎移植或培养至囊胚形成,观察复苏后卵母细胞受精及发育能力。

Result The spindles of oocytes were abnormal at 25℃ for 20 min, and disappeared at 25℃ for 30 min or at 4℃ for 10 min. 5, 10, 20 and 40 μg/ml Cytochalasin B for 2 h had no effects on the spindles of the oocytes. The spindles of oocytes were abnormal in 20 μmol/L colchicines for 1 h, and disappeared in 40 μmol/L colchicines for 1 h. The structure of spindles were perfect for 1-12 h when the oocytes were fixed in 4% paraformaldehyde.

结果]卵母细胞在25℃、20 min时纺锤体异常,25℃、30 min或4℃、10 min时纺锤体缺失;5、10、20和40μg/ml细胞松弛素B作用2 h未对纺锤体产生影响;卵母细胞在20μmol/L秋水仙素中作用1 h纺锤体异常,40μmol/L时作用1 h纺锤体缺失;在4%多聚甲醛中4℃条件下,卵母细胞纺锤体结构以固定时间为1~12 h效果较好。

A correction factor for vitellogenic atresia was included based on the level of atresia and the size of atretic oocytes in relation to normal oocytes finding that OPD would be biased when smaller atretic oocytes are present but not accounted for.

卵黄闭锁的相关因素包括闭锁水平和与正常卵母细胞相关的闭锁卵母细胞的大小,发现当较小的闭锁卵母细胞出现而不计算在内时 OPD 会有偏差。

After that, the comparison of the fertilizable competences of ICSI and IVF on cryopreservated oocytes was studied in this article, in order to establish an optimized method to improve the fertilizable competences of oocytes up to the level of embryos.1 Effects of the reducing injury methods on IVF of cryopreservated oocytesThe result of the three methods, choosing the right time oocytes, using minimum size sample vitrification and culturing after thawing, showed that these ways could partly maintained the fertilizable competences of cryopreservated bovine oocytes.

减轻损伤方案对冷冻卵母细胞IVF的影响使用选择合适时期的卵母细胞进行冷冻;采用最小样本量玻璃化冷冻方法进行冷冻;在解冻后先孵育再进行体外受精等三种方案能够部分维持或恢复冷冻牛卵母细胞IVF受精能力。

Xpression of TGFαand EGFR protein in oocytes, preimplantation embryos and reproductive tracts by immunohistochemy 2.1 Expression of TGFαand EGFR protein in oocytes and preimplantation embryos The results showed that expression of EGFR and TGFαwere in immatured and matured oocytes, cumulus cells, fertilized oocytes and different stage of preimplantation embryos.

GFR和TGFα在未成熟卵母细胞、卵丘细胞和成熟卵母细胞的细胞膜区域均有表达,TGFα在受精卵中的表达量明显增加。

The results showed that : 1 During nonbreeding season, the giant panda′s ovary was still rich with large and small follicular oocytes, and the large follicular oocytes could be matured in vitro; 2 The mean diameter of the oocytes was (141±6.7)μm and lots of cortical granules contained in the cytoplasm of oo...

结果表明∶(1)在非繁殖季节,大熊猫卵巢中小腔及大腔卵泡卵母细胞仍较丰富,其大腔卵泡卵母细胞可在体外培养成熟;(2)大熊猫的卵母细胞平均直径为(141±6.7)μm ,其胞质富含卵黄颗粒;(3)自配 PandaI I号获能液(含丙酮酸钠、肾上腺素等)可使大熊猫精子有效获能;(4)大熊猫卵母细胞体外受精后,经培养可观察到第2 极体,有的受精卵开始发生卵裂。

The result showed that different concentration of FSH and LH were needed for sheep oocytes IVM in different season Xinjiang. FSH 12.9ug/ml,LH0.86IU/ml is proper to sheep oocytes IVM in summer-autumn anestrous period. FSH 8.6ug/ml,LH0.57IU/ml is proper to sheep oocytes IVM in autumn oestrous period.

结果表明:新疆绵羊卵母细胞在不同的季节中,体外成熟所需的FSH、LH浓度不同,夏秋乏情季节适宜于体外成熟的激素浓度为FSH 12.9ug/ml,LH0.86IU/ml;秋季发情季节适宜于体外成熟的激素浓度为FSH 8.6ug/ml,LH0.57IU/ml;春季乏情季节适宜于体外成熟的激素浓度为FSH 8.6ug/ml,LH0.86IU/ml。

The results showed that the rates of mature oocyte were 75.2%, 73.1%, 69.8%, 63.5%and oocyte at telophase of MI were 16.3%, 15.9%, 16.9%, 27.0%, respectively. The rates of maturation of oocytes cultured in TCM199 with serum, EGF and TGFαwere significantly higher than that of with BSA team (P<0.05) and the number of oocyte stayed at telophase of MI in TCM199 with serum, EGF and TGFαteam were significantly less than that with BSA team(P<0.05); Significant higher rates (66.6%, 66.6%, 73.6%) of normalα-tubulin distribution in oocytes cultured in TCM199+ serum, EGF and TGFαwere compared to that of TCM199+BSA(43.3%)(P<0.05). The rates of oocyte with cortical granules in cortex were 58.8%, 33.9%, 54.7%and 47.9%respectively, there was significant difference between oocytes cultured with serum, EGF and BSA(P<0.05). In conclusion, TGFαand EGF can promote the oocyte nuclear transition from telophaseⅠto metaphase of meiosisⅡ, and improved the expression and distribution ofα-tubulin during the ovine oocytes maturation; EGF and serum could promote oocyte cytoplasm maturation. The results suggested that EGF and TGFαmight substitute some substance in serum to improve the quality of oocyte nucleus maturation in vitro, but EGF might be more functional than TGFαto promote the maturation of ovine oocyte ooplasm.

三、EGF和TGFα对卵母细胞成熟的影响未成熟卵母细胞分别在TCM199基础培养液+血清、TCM199基础培养液+BSA+EGF、TCM199基础培养液+BSA+TGFα和TCM199基础培养液+BSA四组成熟培养系统中成熟培养,22小时后上述各组卵母细胞的核成熟率分别为75.2%、73.1%、69.8%、63.5%,处于第一次减数分裂末期的比率分别为16.3%、15.9%、16.9%、27.0%,在添加血清、EGF、TGFα各组中核成熟率显著高于其它各组(P<0.05),处于末期的比例明显低于其它各组(P<0.05):添加血清、EGF、TGFα各组在a-Tubulin蛋白的分布与表达上(66.6%、66.6%、73.6%)明显高于BSA组的正常率43.3%(P<0.05);各组CG发生迁移较好的卵母细胞的比率分别为58.8%、33.9%、54.7%、47.9%,血清组和添加EGF组CG迁移至皮质区的比例明显高于仅添加BSA处理组(P<0.05)。

To pronuclear formation rate after oocyte in vitro maturation 18 hours and parthenogenticactivation, oocytes derived from SM is significantly higher than oocytes from PM,oocytes derived from IM is significantly lower that of oocytes detrived from PM and SM.

对于孤雌刺激反应而言,无论是何种类型的卵母细胞成熟18小时后,自发成熟卵母细胞激活后形成原核的比率高于被动成熟的卵母细胞,诱导成熟的卵母细胞的原核形成率显著低于自发和/或被动成熟的卵母细胞。

The present study is involved in the expression of vitronectin , a ligand of integrin, on human spermatozoa and its role in fertilization; the expression of integrin α〓、α〓、β〓 sbunits on human and mouse oocytes and their relationship with maturation of oocytes; the reorganization of cytoskeletal protein, actin, of mouse oocytes mediated with RGD peptide binding with integrins; the roles and mechanisms of integrins in activation of mouse oocytes as trasmembrane signaling receptors, using the methos of cell culture, immunohistochemistry, flow cytometer, laser confocal microscope.

本课题采用细胞培养、免疫组化、流式细胞术、激光共聚焦显微技术进行以下四个方面的研究:整合素配体玻连蛋白在人精子的表达及其与精子功能状态及受精的关系:整合素〓在人、小鼠卵的表达及其与卵的成熟程度的关系:整合素介导小鼠卵内actin骨架蛋白的重组;整合素介导卵激活的早、晚期事件,包括卵细胞内钙离子浓度的变化及孤雌发育胚的形成,并探讨其机制。

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