查询词典 oocyte

In the present study, we collected cumulus cells oocyte complex from ovaries of two different strain mice. The cumulusenclosed oocytes were cultured for 6 h in MEM supplemented with growth factor and FSH. The meiotic maturation of these oocytes has progressed to pro-metaphse Ⅰ stage and the condensed chromosomes are visible under DIC microscope, metaphase Ⅰ spindle even can be detected under Polscope. The metaphase Ⅰ spindles of oocytes were exchanged under such microscopes. After electric stimuli, 91. 6% and 91. 6% karyoplasts-cytoplasm pairs were fused respectively. The resulting oocytes were cultured further in MEM and over 80% of oocytes released the first polar body. 79% and 77% of oocytes formed two pronuclei after in vitro fertilization and the embryos were cultured in KSOM supplemented with amino acids. Over 60% of embryos developed to blastocyst stage.

在本研究中我们在取得两种不同品系小鼠的卵丘卵母细胞复合体后，先将卵丘卵母细胞复合体置于含有多种生长因子和激素的MEM培养液中培养6小时，此时卵母细胞已进入第一次减数分裂的前中期，并且在DIC倒置显微镜下可以看到浓缩的染色体，用Polscope可以发现明显的纺锤体，借助这种显微镜通过显微操作将两种不同品系小鼠来源的卵母细胞的MI纺锤体进行互换，经过三次直流电脉冲作用后，分别有91.6％的胞质—MI核质体对融合，经过进一步的培养后，超过80％的重组卵母细胞排出第一极体，体外受精后分别有79％和77％的重组卵形成双原核，受精后的胚胎在KSOM胚胎培养液中体外培养4天后，超过60％的胚胎发育至囊胚。

During the course of mice ICSI,some critical factors were realized on influencing the experimental results, including micromanipulation systems, diameters of injection pipettes, concentrations of PVP and positions of the first polar body located in oocyte atlCSL

另外，本研究论文还研究了一些对显微注射结果有影响的因素，包括操作液PVP液的浓度、显微注射时卵母细胞第一极体的位置以及显微操作系统等。

4H was the best time of bovine oocyte activationin the 6-DAMP, and the cleavage rates and blastula rates could reach 85.1% and 26.8%. 4. IVF-100 was used in vitro fertilization, because IVF-100 was not needed to prepare usually,store conveniently, and gain a higher cleavage rates. After the oocytes have matured for 20h, theywere digested by permease and treated with suctionpipe.

由于IVF-100无需经常配制、保存方便且可以获得较高的卵裂率，所以本实验采用IVF-100为受精液，卵母细胞成熟20小时后采用透酶+口吸管消化的方法处理，获得了较好的受精效果，卵裂率可达(76.8％)。

We killed some mice at the day 6th , the others were continue to expose to formaldehyde and observe their estrous cycle. HE staining was used to study the pathological changes happened in ovarian tissue. Uranium acetate and lead acetate double staining was used to study the ultrastructure of oocyte. E2 and LH level in blood serum was measured by radioimrnunoassay reagent kit.

第6d处死一批小鼠，用HE染色观察小鼠卵巢组织的病理改变，用醋酸铀—醋酸铅双重染色电镜下观察小鼠卵母细胞超微结构的损伤；用GSH测定试剂盒测定小鼠卵巢组织中的GSH含量；用E_2和LH放射免疫分析试剂盒分别测定小鼠血清中E_2及LH水平；采用超排卵法，计数超排卵数并收集卵母细胞，于油镜下观察生殖细胞遗传物质的损伤。

The results showed that the insulin-induced oocyte maturation was accompanied by the decreases in membrane potential, K+ transference number and intracellular K+ and H+ activities, and the increases in intracellular Na+ and Cl- activities.

Malle：等(198们的实验也证实了胰岛素及其类似物的这种作用。Lessman等〔1984)曾报道，胰岛素在促进卵母细胞成熟同时，膜的某些特性发生了变化，如膜阻抗增加，膜电容减少和膜电位降低去极化〕等。

Membrane potential, intracellular Na+, K+, Cl, H+ activities and Na+, K+ transference number in toad oocytes were measured in vitro by using conventional and ion-sensitive microelectrodes during the insulin-induced oocyte maturation.

近年来，胰岛素及胰岛素样肤类物质在卵母细胞成熟中的作用越来越引起人们的关注。EI一Etr等(1979)用非洲爪蟾卵母细胞作实验，发现胰岛素及其类似物具有促进卵母细胞成熟分裂的作用。

Four main factors which possibly affected sex were described such as arginine concentration in female animal vagina, glucose in the uterus, maturational status of the oocyte at the time they encounter sperm as well as the hormone levels of both parents at the time of conception.

这些环境因素包括母体生殖道中精氨酸含量、胚胎发育过程中子宫内葡萄糖浓度、输精时卵母细胞成熟程度以及哺乳动物受孕时生殖激素的水平等方面，为进一步开展性别控制研究积累一定的资料。

The germinal vesicle can beisolated successfully in such a physical method and it can undergo chromatincondensation in vitro just as the oocyte does in vivo.

用直接取核法可成功分离出游离的生发泡，且它们在体外培养条件下可以和体内一样发生染色质凝集现象。

Inject PAK2-N teminal (PAK2-NT) mRNA to Xenopus oocyte,and observe germinal vesicle breakdown by fluorescence microscope.

荧光显微镜下比较PAK2-NT mRNA注射组和未注射对照组卵母细胞胚泡破裂发生。

The results showed that chromium trichloride could inhibit the extruding of the first polar body and affect the quality and the viability of mouse oocyte and reduce the rate of IVF and the number of oocytes of superovulation, inhibit the germinal vesicle breakdown of mouse oocytes in vitro culture, but little impact on GVBD of mouse oocytes in vivo .

结果表明，三氯化铬可以抑制卵母细胞第一极体的释放，降低小鼠超排卵数和卵母细胞的存活率和体外受精率。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。