查询词典 oocyte

Mouse zygotic genome is activated at the two-cell stage which implies that embryonic development is transferred from the oocyte itself to the embryo.

在小鼠胚胎发育研究中发现，小鼠的基因组激活发生在2细胞期，这一时期标志着合子的发育由卵母细胞控制向胚胎控制的过渡，期间发生一系列复杂的生化过程。

Embryo cells display a synchron cleavage rhythm during the cleavage stage, there is almost no gene transactivation, which is regulate and operated by the mRNAs and protein of oocyte.

卵裂阶段的胚胎细胞呈现同步化的分裂节律，鲜有基因转录活动，其发育由源自卵母细胞发生期间合成的蛋白质和mRNAs调控。

Enucleated metaphase Ⅱ oocyte can support reprogramming of transferred nucleus and further development to term.

许多实验证明去核的MⅡ期卵母细胞可支持移入的核进行重新编程并发育到出生。

In order to establish mouse nuclei transfer technology, mouse zygote culture medium and activated conditions were optimized in vitro and enucleated method of MII oocyte were investigated in this study.

本研究通过对小鼠受精卵体外培养体系，MII期卵母细胞孤雌激活条件的筛选以及探讨MII期卵母细胞的去核方法为小鼠体细胞核移植的成功奠定基础。

Howeer, at least one group has made it to the first step--creating a human blastocyst by inserting the nucleus of an adult skin cell into an enucleated oocyte Science, 28 April, p.

然而，至少有一个研究小组已经走出了第一步，通过将一枚**皮肤细胞的细胞核植入一枚去核的卵细胞培育了一个人类胚泡（科学，4月28日，516页）。

Under the transmission election microscope, we had observed that the state of oocytes arid the cumulus cells around them was tightness, and there were plenty of long and thin microvilli playing the role of connection in the enwrapping. The shape of cumulus cells nucleus was ovate, and the longer and shorter diameters of the cumulus cells were 4.250±0.042μm and 2.750±0.07 μm respectively. The zona pellucida was well proportioned and tightly linking with oocyte. There were great deals of multi-crista mitochondria, lipid droplet and vacuolus. The mitochondria longer and shorter diameters were 0.600±0.106μm and 0.490±0.117μm. Granular nucleoplasma distributed very well in the cell nucleus.

电镜下，卵母细胞外的卵丘细胞紧紧包裹卵母细胞，且与卵母细胞之间有大量细长的微绒毛相联系；卵丘细胞核呈卵圆形，其长径为4250±0.042μm，短径为2.750±0.071μm；透明带均匀，与卵母细胞结合紧密；在皮质区集中分布大量的多峰型线粗体、脂滴和空泡，线拉体长径为0.600±0.106μm，短径为0.490±0.117μm；细胞核中颗粒状的核质分布非常均匀。

Methods Human luteinized granulosa cells were isolated from follicular fluid obtained during oocyte retrieval of in vitro fertilization-embryo transfer program and were cultured with M199 medium plus various concentration of leptin (0, 10, 30, 100, 300 ng/ml),human menopausal gonadotropin (hMG, 0, 0.1, 0.2, 0.5, 1, 2, 5, 10 IU/ml) and testosterone 100 μg/ml. For 2 days the media were collected for estradiol and progesterone measurements.

方法将来自体外受精-胚胎移植的卵巢黄素化颗粒细胞纯化后，在不同浓度瘦素(0、10、30、100、300 ng/ml)和人绝经期促性腺激素（hMG，0、0.1、0.2、0.5、1、2、5、10 IU/ml）单独或联合作用下进行体外培养，收集培养液，采用放射免疫方法测定颗粒细胞产生雌二醇及孕酮的量。

Methods Human luteinized granulosa cells were isolated from follicular fluid obtained during oocyte retrieval of IVF-ET program and were cultured with M199 media plus various concentration of follicle stimulating hormone (FSH, 0% 0.5% 5% 10% 25% 50) or human chorionic gonadotropin(hCG, 0% 5% 10% 50% 75% 100IU/ml). The media were collected and PAPP-A in the media were measured by time-resolved fluoroimmunoassay.

将来自体外受精—胚胎移植的卵泡黄素化颗粒细胞分离纯化后，利用不同剂量促卵泡激素(FSH，0、0.5、5、10、25、50)或人绒毛膜促性腺激素(hCG，0、5、10、50、75、100IU／ml)刺激体外培养的颗粒细胞，收集培养液，采用时间荧光免疫分析法测定培养液中PAPP-A的量。

Indirect immunofluorescence was used to observe the distribution of fibronectin in human oocyte and 2-cell to morulae embryos.

纤维粘连蛋白均匀分布在体外未受精人卵母细胞和受精后人胚胎早期发育的不同阶段的卵裂球表面，各时期的免疫荧光强度未见明显差别。透明带未见纤维粘连蛋白表达。

The distribution of fibronectin in human oocyte and 2-cell to morulae embryos was also investigated, and the possible roles of fibronectin in fertilization, the development of early embryo and implantation were also discussed.

应用免疫组织化学方法和图像分析技术研究整合素β_3、FN和ER、PR在输卵管粘膜内的表达和变化；采用间接免疫荧光技术对FN在人卵母细胞和早期胚胎内的表达分布进行观察；光镜观察输卵管粘膜上皮细胞的形态学变化。结果：1。整合素β_3在人正常输卵管粘膜上皮纤毛细胞的胞浆内表达。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。