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Dan Millman: The ones who are hardest to love are usually the ones who need it the most.

丹：让人最难去爱的人往往是最需要爱的。

Part I :the definition of officialdom novels ,the insightful discussion of the development of them ,and the primary analysis of the reasons of their flourishing ;Part II :the construction of subject matters of officialdom novels by probing analysis into official corruption ,official and social psychologies ;Part III: the of artistic features of characters and narrative (i.e. narrative perspective constructions ,discourse and ironical narration ),which are illustrated by the positive characters with personalities ,negative ones with complex characteristics ,more importantly ,the vulgar existence ofgray characters ,the discovery of features of narrative methods with the contract of two types of novels ; Part IV: the positive comment on the realistic rules of officialdom novels and the surpassing of the previous ones ,the shortcomings from the artistic perspective : the adoption to the reality seriously fetters artistic imagination of literature works ,it also reads to the lacking of insightful thinking ,the simple characters fail to reveal artistic glamour ,the routinized narrative methods also degrades the artistic savor of works .

第一部分，界定官场小说的内涵，并对它的历史发展，对其在当下繁荣的原因做初步的探析；第二部分，从官场小说对官场腐败、官员内心世界和社会文化心理等方面的揭示考察其主题构成；第三部分，从人物形象、叙事方式(叙事视角、叙事结构、反讽叙事、叙事话语)等分析官场小说的艺术表现特征，指出官场小说不仅塑造了性格鲜明的正面人物，也真实刻画了具有复杂人性的反面角色，更重要的是展现了官场灰色人物的庸俗生存，并通过两类不同类型小说的对比，揭示其各自不同的叙事特征；第四部分，充分肯定官场小说的现实主义价值，及其对前期官场小说的超越，并从小说艺术角度指出其存在的严重不足：小说对现实的屈从束缚了作品的艺术想象力，使小说缺乏深厚的思想内蕴；人物形象也显得单薄，无法产生感人的艺术魅力；叙事的模式化也大大降低了作品的艺术品位。

Except on this view, at any rate, there will be two kinds of ultimate normative issues, moral ones and nonmoral ones.

除了关于这种观点，至少将有两类基本的规范性问题，道德的一类和非道德的一类。

This paper gave a detailed introduction of the various kinds of free information provided by tourist information centers at tourism destinations in the United States of America, sorted them into official and nonofficial ones according to the publishers and property rights and map information and picture-and-text information ones according to their contents.

本文详细介绍了美国旅游地游客信息中心提供的各类免费信息，根据出版者和版权属性将他们区分为官方与非官方信息，根据内容将他们区分为地图信息和图文信息。

When they fed on malaria-infected mice, the resistant mosquitoes had a higher survival rate than nonresistant ones, meaning they could eventually replace the ones that can carry the disease, according to a report in Tuesday's issue of Proceedings of the National Academy of Sciences.

据星期四国家科学研究院的最新报告，研究人员培育抗疟疾老鼠时发现，抗疟疾蚊子比非抗疟疾蚊子成活率要高得多，这意味着抗疟疾蚊最终必将取代非抗疟疾蚊。

Then he used 'obduracy' rather than 'enthusiasm', however, while the testees were asked to imagine again, the result was they overturned the former ones to a totally different ones.

再要求被试者根据这五种品质想象出一个形象时，却发现被试者普遍推翻了原来的形象，而产生了一个完全不同的形象。

In August 1957, TOYOTA export cars to America at first time, in the same year set up "American TOYOTA car selling company" in Los Angeles, at same time America produce 7 million and 220 thousands cars, sell 6 million and 930 thousands ones, but In Japan produce 180 thousands cars, sell 160 thousands ones, less than oddments of sales quantity of America cars.

这一年，美国年产汽车722万辆，销售693万辆，而日本生产汽车只有区区18万辆，销售16万辆，还不及美国一个小小的零头。

Positive ones in biology favors "new" while negative ones are just the opposite, so positive biology is outrunner of evolution.

生物界中的阳者喜欢"新"，而阴者反之，故喜新恶旧的阳者生物在进化中起先驱作用。

A community may outgrow its hospitals and schools and look forward to building new ones, but in the case of prisons and jails, the tendency has been to expand or to overcrowd existing buildings rather than to build new ones.

一个社区可能会因为医院与学校不够用而期望新建一些，但对于监狱而言，总是倾向于扩建或者保持不变（在现有的空间下挤入更多的囚犯），而不是建造新的监狱。

RESULTS A eukaryotic expression system for high expression humanmutantCD59 were successfully set up : The recombinant PALTER-MAX plasmid containing human mutantCD59 cDNA and PCDNA plasmid were co-transfected into CHO cell by cation lipoid mediating method ;and the cells were grown in F12 medium containing 400ug/ml G418 for 14 days, positive clones were grown in RPMI1640 medium to get stable expressing cell lines . Highly expressing clones were selected by flow cytometry ,and were named PALTER-CD59-CHO1PALTER-CD59-CHO2 . Flow cytometry indicated that expression rates of PALTER-CD59-CHO1 and PALTER-CD59-CHO2 were 53.7%and 54.5%. Further more, Stable highly expressing CHO cell lines were more detected by immunocytochemistry and immunofluorescence technology . PALTER-CD59 -CHO1 and PALTER-CD59-CHO2 were grown in RPMI1640 to get a large of cells . CD59 protein were obtained by spalling PALTER- CD59- CHO1 and PALTER - CD59 - CHO2 cells . Stable highly expressing cells were further validated by SDS-PAGE, immunoblot analysis and solid enzyme immunoassay . PALTER - CD59 - CHO1 and PALTER - CD59 - CHO2 were glycated in RPMI1640 of 50mM ribose for 72 hours .BCECF releasing test indicted that the releasing rate of PALTER-CD59-CHO1 or PALTER-CD59-CHO2 was less high than PALTER-CHO ,and the releasing rate of glycated PALTER-CD59 -CHOI or PALTER-CD59-CHO2 was higher than unglycated ones . PALTER -CD59-CHO1 and PALTER -CD59 -CHO2 were glycated in RPMI1640 of 50mM ribose for 72 hours .BCECF releasing test indicted that the releasing rate of PALTER - CD59 - CHOI or PALTER - CD59 - CHO2 was less high than PALTER-CHO ,and the releasing rate of glycated PALTER-CD59-CHO1 or PALTER - CD5 9-CHO2 was higher than unglycated ones .

结果 成功构建突变人CD59的真核细胞表达系统：运用阳离子脂质体介导法将含有突变人CD59的PALTER—MAX重组质粒与PCDNA共转染入CHO细胞：用含有400ug／mlG418的F12培养基培养14天，筛选出稳定阳性表达克隆，RPMI1640培养基扩增获得稳定表达细胞株，并用流式细胞术进一步筛选出高效表达细胞株分别命名为PALTER—CD59—CH01、PALTER—CD59—CH02，表达率分别为53.7％、54.5％；应用免疫组化方法、免疫荧光技术进一步鉴定阳性细胞株；RPMI1640培养基大量扩增PALTER—CD59—CH01、PALTER—CD59—CH02细胞株，裂解细胞得到CD59蛋白质；通过SDS—PAGE凝胶电泳技术、免疫印迹技术、固相酶联免疫吸附试验验证了这两中文摘要个阳性细胞株CO59蛋白的高效表达；50mM核糖培养72小时，获得突变人CD59糖化细胞株，BCECF染料释放试验结果显示，PALTER一CD59一CHOI、pALTER一CD59一CHOZ细胞较PALTER一CHO细胞染料释放率低，未糖化PALTER一CD59一CHOI、PALTER一CD59一CHOZ细胞比较糖化后细胞染料释放率低。

Neither the killing of Mr Zarqawi nor any breakthrough on the political front will stop the insurgency and the fratricidal murders in their tracks.

在对危险的南部地区访问时，他斥责什叶派民兵领导人对中央集权的挑衅行为。

In fact,I've got him on the satellite mobile right now.

实际上 我们已接通卫星可视电话了