查询词典 one-step method

Using large sample DNA of capacity technology, the maize high lysine content of SSR mark analyse technological platform has been established in this paper. The main results are as follows: the five comparing research methods of drawing maize DNA mark have shown that the method of iso- sulphur cyanic acid guanidine drawing maize genome DNA has the superiority of using less medicine, operating sequence more simple , faster, and costs lower , steady and reliable quality of DNA, etc..Utilizing this method , one person can draw 300-400 DNA samples each workday.lt indicates that the method can be used in SSR marker of maize,.

本文从大样本容量的DNA快速提取技术入手，初步建立了玉米高赖氨酸含量的SSR分子标记分析技术平台，获得的主要结果如下：对五种玉米DNA提取方法的比较研究认为，异硫氰酸胍法提取玉米基因组DNA，具有使用药品少、操作步骤简单、快速、成本低、DNA质量稳定可靠等优点，利用该方法，每人每个工作日可以提取300～400个DNA样品，可以在玉米SSR标记分析中应用。

Firstbrook expresses, those rubbish mail maker people because very possible meeting wants not tire of to its register Google Docs account irritatedly and abandon mail of this one rubbish producing a method, because Gu Ge adopted a kind of effective ground to prevent to register the method of the account automatically, if they are used after Google Docs account is dropped by screen, they the method that must use handiwork to register.

Firstbrook表示，那些垃圾邮件制造者们很有可能会因为要不厌其烦地注册Google Docs帐户而放弃这一垃圾邮件制造方法，由于谷歌采取了一种有效地防止自动注册帐号的方法，假如他们使用的Google Docs帐号被屏蔽掉后，他们将不得不采用手工注册的方法。

In this article, anthrone-sulfuric method and phenol-sulfuric method were used to test quantificationally Medicago sativa polysaccharides content, and made sure which one is the better method.

采用蒽酮－硫酸法和苯酚－硫酸法测定了苜蓿芽多糖含量，并比较了2种方法的适用性。

The proposed method contains two main parts: one part is determination of the noncontinuous factors by the orthogonal design method and the other is determination of the continuous factors by the uniform design method.

联合试验设计法主要分为：用正交试验设计法确定非连续性因素及其在备选水平中的适宜水平，以及用均匀试验设计确定连续性因素及其备选水平中适宜的水平范围两部分。

We found out one kind of new orange-red long afterglow material La2O2S: Eu3+, Mg, Zr.2 In the phosphor Gd2O2S, Mg, Ti which was prepared by high-temperature solid-state method, the orange afterglow phenomenon can be seen after illuminated with UV light.3 Using the high-temperature solid-state method, Gadolinium oxysulfide phosphors doped with rare earth ions were synthesized. We can observe the red or orange phosphorescence in Gd2O2S doped with Eu3+, Sm3+, Tm3+, Yb3+, Dy3+, Er3+, Nd3+or Ho3+. This is a new result of the long afterglow phosphors research.4 Using the microwave radiation method, the new Sm3+ doped gadolinium oxysulfide phosphor, which has the orange-red long afterglow emission after illuminated with UV light or visible light, was synthesized for the first time.5 In fluorescent materials YOX doped with europium ions, the long afterglow phenomenon wasnt observed but the blue luminescence of Eu2+ and red luminescence of Eu3+ are simultaneously examined. This is a new result of the valence stability and change of Eu2+ in yttrium oxyhalide .We systematically studied the effects of the host, addition agents and reaction atmosphere on the valence change and fluorescence property of europium ions.

经过系统的研究总结得到以下的结论与成果： 1采用高温固相反应法合成了一系列稀土离子掺杂的硫氧化镧发光粉，发现了该体系中Eu~(3+)的长余辉发光，从而得到一种迄今未见文献报道的红色长余辉发光材料：La_2O_2S：Eu~(3+)，Mg，Zr； 2在Gd_2O_2S，Mg，Ti磷光粉中观察到橙色的长余辉发光现象； 3采用高温固相反应法合成了一系列稀土离子掺杂的硫氧化钆磷光粉，在掺杂Eu~(3+)、Sm~(3+)、Tm~(3+)、Yb~(3+)、Dy~(3+)、Er~(3+)、Nd~(3+)、Ho~(3+)的Gd_2O_2S中观察到红色和橙色长余辉发光； 4首次采用微波法合成了Gd_2O_2S：Sm~(3+)，Mg，Ti磷光粉，该磷光粉经紫外或可见光激发后呈现明显的橙红色长余辉发光； 5在YOX中同时观察到Eu~(2+)和Eu~(3+)的发射，系统研究了基质、添加剂及反应气氛对Eu价态和发光的影响。

The available methods of optial system in emulating system are compared with each other in this paper and the photo electrical measurement method is accepted to be the best one.Then,the practical scheme of photoelectrical measurement method and its mathematical models are given,and the measurement error of this method is also analyzed qualitatively.

本文对仿真系统中的光学系统的可能实现方法进行了比较，选择出其中较优秀的方法—光电测量法，给出了光电测量方法的实际实现方案和测量的数学模型，对此方法的测量误差进行了定性分析。

The comparison test of this new methodwith spray inoculation method was conducted. The results showed the former method could increase the incidence ofdisease and uniformity than the latter one. Used cotyledon-brushing with conidial suspension and fungicide foliarspraying method, the EC 50 values of hexaconazole, myclobutanil, triadimefon, thiophanate-methyl and chlorotha lonil to Sphaerotheca fuliginea treated in different time after inoculation were determined; the results showed that 96 h was better time for disease severity evaluating.

比较了白粉病菌分生孢子悬浮液涂抹法和喷雾法接种黄瓜幼苗子叶的效果，结果表明，涂抹法发病率高，均匀度更好；测定了接菌后不同时间施药，白粉病菌对己唑醇、腈菌唑、三唑酮、甲基硫菌灵和百菌清等 5 种杀菌剂的敏感性，结果表明，接菌后 96h 施药较为敏感，测得的 EC 50 较小。

On the basis of URNA's characteristics, psychophysical method, one of the most scientific and credible methods, is chosen to establish the complete landscape assessment system, including acquiring scenic beauty value by SBE method, dividing into different influencing factors by SD method, and setting up their connection model by multiple linear regression analysis.

城市自然遗留地是随着城市化的进程在城市规划区内遗留下来的的用地，根据城市自然遗留地城市性、自然性和遗留性的特点，结合国内的实际情况，运用目前最严格、可靠性最好的心理物理学方法，以相片为测试材料，采用SBE法获得美景度量值，SD法测定各景观要素量值，以及多元线性回归方法建立两者的关系模型，最终提出了城市自然遗留地景观美学评价完整的方法体系，并修正和完善了心理物理学方法在城市自然遗留地领域的具体应用。

In this article, method of moment and Floquet mode method will be applied to analysis the radiation and scattering of planar array and curved array. As one part of the work, the induced current on perfect conductor surface is computed with MoM method and graphed by Origin.

本文分别采用比较经典的低频方法—矩量法和一种专用于阵列分析的高频方法—Floquet模(FM，Floquet Mode)方法对有限阵列电磁散射特性进行分析。

ABSTRACT It is because that the excellent acceptors for maize transgenic engineering are insufficient in our country, especially in the southwest mountain areas of china and hereditary variation regularity for the two characters such as efficiency of embryonic callus induction and number of regenerating plant (these two characters were abbreviated to the nduction efficiency and number of regenerating in the following of the paper, respectively), which hint the maize culturing capacity, is not very clear. Therefore, aiming at picking out superior acceptors, we had made systematic researches on the two characters with combing traditional quantitative-character genetic analyzing methods such as single-factor genetic mating design, diallel crossing genetic design, genetic effect analyzing method and the modern molecular locating method such as QTLs'. The main results are followed.(1) 50 superior inbred lines and about 30 crosses in our country, especially in the southwest of China were used for identifying and selecting the superior genotypes in the above two investigated characters under the same culturing condition in 2000 and 2001. There was very significant difference among the genotypes in the both characters. But the two characters were not certainly related. Some genotypes such as 18-599 and 18-599 were very good in them. For some ones such as zong 31, induction was higher than 18-599 and 18-599 in the efficiency, but it was only 1/3 to the later in regenerating number. In some genotypes such as S37, R08, R15, P138, A318, induction efficiency was just about 3% and scarcely any regenerating plants were got. On the whole, hybrids acted better than inbreeds in the both characters.(2) Two kind of inbreeds were selected as parents of the Griffing's method 1. 18-599 and 18-599 and the inbred line zong 31 are one kind because they are not only superior in the characters of maize cross breeding, such as CA, resistance to disease and the important agricultural characters, but also excellent in transformation characters as the induction and regeneration.

针对我国、特别是西南山地所需玉米转基因工程育种优良受体极为匮乏和反应玉米幼胚培养能力的2个主要性状，即玉米幼胚胚性愈伤组织诱导率和胚性愈伤组织绿苗发生数的遗传变异规律十分不清楚的实际情况，本研究从筛选玉米转基因工程所需要的优良受体入手，采用单因素遗传交配设计、双列杂交遗传交配设计、世代基因效应等传统数量性状分析方法，以及现代分子标记定位主效QTL分析方法，对玉米幼胚胚性愈伤组织诱导率和胚性愈伤组织绿苗发生数等2个性状进行了较为系统的分析研究，取得以下主要研究结果：(1)于2000年和2001年通过对我国、特别是西南地区近50份优良自交系和近30个杂交组合，在相同培养条件下，对幼胚培养胚性愈伤组织诱导率和胚性愈伤组织绿苗发生数等2个幼胚培养能力性状进行了筛选与鉴定，发现玉米不同基因型具有完全不同的幼胚培养胚性愈伤组织诱导率和胚性愈伤组织绿苗发生数，但幼胚培养胚性愈伤组织诱导率与胚性愈伤组织绿苗发生数并不具有必然的相关关系，有的基因型，如自交系18-599和18-599在胚性愈伤组织诱导率和愈伤组织绿苗发生数等2个性状都表现相当优异；有的基因型，如自交系综31，仅幼胚培养胚性愈伤组织诱导率性状表现高于19-599和18-599，但在胚性愈伤组织绿苗发生数这一性状则与它们有相当大的差距，仅为19-599和18-599的1/3左右；有的基因型，如S37、R08、R15、P138、A318等玉米自交系不仅幼胚培养胚性愈伤组织诱导率很低，平均仅在3％左右，而且胚性愈伤组织绿苗发生数表现也很差，基本上没有分化成苗。

As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸，那乌黑、忧郁的眼睛，她便会相信，他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失