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The present invention relates to a process for rapid solution synthesis of a peptide, the process comprising repetitive cycles of steps-: a coupling step, using an excess of an activated carboxylic component to acylate an amino component, a quenching step in which a scavenger is used to remove residual activated carboxylic functions, wherein the scavenger may also be used for deprotection of the growing peptide, one or more aqueous extractions and optionally, a separate deprotection step, followed by one or more aqueous extractions, characterised in that the process comprises at least one step, referred to as step, in which an amine or a thiol comprising a free anion or a latent anion is used as a scavenger of residual activated carboxylic functions.
本发明涉及快速溶液合成肽的方法,该方法包括步骤-的重复循环:偶联步骤,使用过量的活化羧基成分酰化氨基成分,淬灭步骤,在该步骤中使用清除剂除去残余的活化羧基官能团,其中该清除剂还可以用于使生长中的肽去保护,一或多个水抽提和任选地,独立的去保护步骤,之后进行一或多次水抽提,其特征在于该方法包括至少一个步骤,称作步骤,在该步骤中包含自由阴离子或潜阴离子的胺或硫醇用作残余活化羧基官能团的清除剂。
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The highest seajiao xiao Suona flute sound, long strokesliu cun ; Suona treble flute called material, under about a foot; Alto flute solo show called Luo,er cun rod about a foot; Suona Tenor II called the first mixed, under foot by one inch in length; Suona bass flute called big stuffy, under one foot three-inch to one foot longwu cun .
最高音唢呐叫小海笛,杆长约六寸;高音唢呐叫材笛,杆长约一尺;中音唢呐叫罗戏笛,杆长约一尺二寸;次中音唢呐叫二混头,杆长约一尺一寸;低音唢呐叫大闷笛,杆长一尺三寸至一尺五寸。
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Questionare about asthenopia was filled by the patients immediately after laser treatment and one day, one week and one month after using the eyedrop. Side effects of the eyedrop were also observed.
对 60例91眼激光治疗后使用七叶洋地黄双苷滴眼液的患者,分别在激光后即刻、用药后1d;1wk;1mo进行视疲劳症状问卷调查,同时观察药物的不良反应。
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Secondly, the determinist can give a rationale for why we must feel free in terms of the conceptual impossibility of replacing one's own practice of deciding by one of merely predicting one's own behavior.
其次,决定论者能给出一个基本原理,这个原理说明为什么我们必须感到自由在概念上的不可能性,这种不可能性是指通过预言一个人的行为来决定替换一个人的自身实践是不可能的。
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Honesty means to be sincere, earnest and selfless, which places emphasis on fact, keeping one's refers to abide by one's word, keep one's promise and be true in word and resolute in deed.
诚实是指真诚不伪,诚信不欺,真实不妄,精诚不懈。其强调的是真实。守信是指遵守自己的诺言,实践自己的诺言,言而有信,行有必果。
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Day I has let me cook meal , the noisy bell has also bloomed,I have felt have sth. on one's mind to be engaged, as soon as do not know having been awake also slept well , got up luckily at 6 o'clock much time , in the evening, have been ready for breakfast for them, 2nd day has broken down , the noisy bell has also been unable to hear, one sleep to 7 o'clock , late meal of sleep still is to be going to compose , just being it is exactly by one point lately.
第一天叫我做饭,闹铃也开了,我感觉心里有事了,一晚上不知道醒了多少次,也没有睡好,幸好6点起来了,给他们做好了早饭,第2天就坏了,闹铃也听不见了,一觉睡到7点,晚了饭还是要做的,只不过是晚吃一点就是了。
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A perquisite is a privilege or advantage accorded to one by virtue of one's position or the needs of one's employment
Perguisite 是指由于某人地位的功效或者其工作需要而享有的特权或利益
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On the one hand, one benzen ring of stilbene was substituted by one pyridine ring for the purpose of evaluating the bioactivities of azastilbenes. On the other hand, some stilbene derivatives with different substituents in different sites were designed for the study of SAR.
一,以吡啶环替代母核的一个苯环,考察分子中引入氮原子对活性的影响;二,改变母核上的取代基类型及取代位点,考察取代基在构效关系中的影响规律。
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The unoperated sides of the treated animals also served as controls. Six normal rats were treated as normal control group. Three different siRNA plasmid solution containing RC2-Ⅰ, MAFbx-Ⅱ, CON (50μl , 0.8μg/μl)was injected and transfected by electroporation as methods mentioned above, respectively. The changes of RC2 and MAFbx mRNA levels and RC2 protein levels after 3 days were determined by real-time quantitative PCR and Western blot, respectively. On postoperative 2, 3 and 4 weeks, the rate of wet muscle weight preservation, mean diameter of muscle fiber and mean cross-section area of muscle fiber and muscle protein content were checked and then compared between group CON and group RC2 or group MAFbx, respectively. The differences between groups were analyzed by one-way ANOVA. Ultrastructural changes of muscle fiber were observed at 2, 3, 4 weeks postoperation.Results GFP plasmid was efficiently deliverd into muscle by electroporation and robust GFP expression in muscle could be observed more than three weeks. Histology shows that injected plasmid DNA diffuses extensively in muscle tissue.
1、健康雌性SD大鼠18只,随机分为电穿孔组和非电穿孔组,每组9只,制作右下肢趾长伸肌失神经支配模型;EP组为将质粒pEGFP-N1溶液50μl(0.8μg/μl)注射入右趾长伸肌后,立即于两侧腱腹交接处给予电穿孔,电穿孔参数为:电场强度为200V/Cm,脉冲100μs,频率1Hz,施加10次脉冲;NEP组仅质粒pEGFP-N1溶液注射;转染后1、2、3周,荧光显微镜下观察趾长伸肌中GFP的表达情况,转染后1周行Western印迹检测趾长伸肌中GFP蛋白的表达情况,检测和优化体内转染效率。2、健康雌性SD大鼠78只,随机分为失神经对照组、RC2基因治疗组(RC2组),MAFbx基因治疗组,每组24只,制作右下肢趾长伸肌失神经支配模型,余6只为正常组;分别将含CON、RC2、MAFbx基因的siRNA重组质粒注射入趾长伸肌,之后给予电穿孔,方法同上;治疗后3天实时定量PCR和Western印迹检测各组中RC2或MAFbx基因的mRNA和蛋白的表达变化,治疗后2、3、4周检测各组肌湿重维持率、肌细胞直径和肌细胞截面积,肌细胞超微结构变化以及肌纤维中蛋白含量变化。
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If it is declared, that R600 have320 streamprocessors, then actuality the chip contains only 64 blocks, each of which has on one scalar and one vector (4D) processor, which consists of four components, controlled by one block.
如果是申报,而且r600已经流320处理器,那么现状芯片只含有64座每有一个标量和一个向量处理器,它由四部分组成,由1座。
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- 推荐网络例句
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We have no common name for a mime of Sophron or Xenarchus and a Socratic Conversation; and we should still be without one even if the imitation in the two instances were in trimeters or elegiacs or some other kind of verse--though it is the way with people to tack on 'poet' to the name of a metre, and talk of elegiac-poets and epic-poets, thinking that they call them poets not by reason of the imitative nature of their work, but indiscriminately by reason of the metre they write in.
索夫农 、森那库斯和苏格拉底式的对话采用的模仿没有一个公共的名称;三音步诗、挽歌体或其他类型的诗的模仿也没有——人们把&诗人&这一名词和格律名称结合到一起,称之为挽歌体诗人或者史诗诗人,他们被称为诗人,似乎只是因为遵守格律写作,而非他们作品的模仿本质。
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The relationship between communicative competence and grammar teaching should be that of the ends and the means.
交际能力和语法的关系应该是目标与途径的关系。
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This is not paper type of business,it's people business,with such huge money involved.
这不是纸上谈兵式的交易,这是人与人的业务,而且涉及金额巨大。