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The stable clones are further identified by RT-PCR and Western blot; 6 MTT assay is used to investigate the effect of ZNRD1 on the cell growth of cells (AGS, SGC7901, MKN28, NIH3T3, GES-1); 7 Soft agar assay is used to investigate the effect of ZNRD1 on the clonality of cells (AGS, MKN28); 8 Nude mice assay is used to investigate the effect of ZNRD1 on the cell growth of gastric cancer cells (AGS, MKN28); 9 Flow cytometry is used to investigate the effect of ZNRD1 on the cell cycle distribution of cells (AGS, MKN28, NIH3T3, GES-1); 10 Flow cytometry is used to investigate the effect of ZNRD1 on the cell apoptosis of cells (AGS, MKN28, NIH3T3); 11 MTT assay is used to investigate the effect of ZNRD1 on the drug sensitivity of cancer cells (SGC7901, SGC7901/VCR, HL-60, HL-60/VCR) in vitro; 12 SRCA is used to investigate the effect of ZNRD1 on the drug sensitivity of gastric cancer cells (SGC7901, SGC7901/VCR) in vivo; 13 Flow cytometry is used to investigate the effect of ZNRD1 on adriamycin accumulation of cancer cells (SGC7901, SGC7901/VCR, HL-60, HL-60/VCR); 14 Transmission electron microscope is used to investigate the effect of ZNRD1 on the sensitivity of SGC7901 cells towards drug-induced apoptosis; 15 Flow cytometry and DNA ladder assay are used to investigate the effect of ZNRD1 on the sensitivity of cells (SGC7901, SGC7901/VCR, HL-60/VCR) towards drug-induced apoptosis; 16 Microarray is used to investigate the profiling of ZNRD1-responsive genes in gastric cancer cells (AGS, MKN28, SGC7901, SGC7901/VCR); 17 RT-PCR and Western blot are used to identify the results of microarray; 18 Reporter gene assay is used to investigate the effect of ZNRD1 on the transcriptional activity of cyclin D1; 19 Reporter gene assay is used to investigate the effect of ZNRD1 on the transcriptional activity of MDR1; 20 Kinase assay is used to investigate the effect of ZNRD1 on the activity of cyclin E-CDK2 kinase; 21 The antisensenucleic acids of p21 is used to inhibit the expression of p21, and flow cytometry is used to investigate the effect of p21 on ZNRD1-induced cell cycle arrest in gastric cancer cells; 22 The antisensenucleic acids of p27 is used to inhibit the expression of p27, and flow cytometry is used to investigate the effect of p27 on ZNRD1-induced cell cycle arrest in gastric cancer cells; 23 Liposome is used to up-regulate the expression of Skp2, and flow cytometry is used to investigate the effect of Skp2 on ZNRD1-induced cell cycle arrest in gastric cancer cells; 24 Western blot is used to investigate the effect of ZNRD1 on the stability of Skp2 and p27 in gastric cancer cells; 25 MVD assay is used to investigate the effect of ZNRD1 on the angiopoietic activity of gastric cancer cells; 26 ELISA is used to investigate the effect of ZNRD1 on the expression of VEGF165 in gastric cancer cells; 27 The roles of DARPP-32 in MDR of gastric cancer cells are investigated using gene transfection, MTT assay, SRCA, flow cytometry and DNA ladder assay.

应用杂交瘤技术制备ZNRD1的首个单克隆抗体；2）利用RT-PCR、Western blot和免疫组化检测ZNRD1在胃癌组织、胃炎组织、正常胃上皮组织、胃癌细胞和正常胃组织上皮细胞中的表达；3）构建ZNRD1的小干扰RNA载体，并测序鉴定；4）利用脂质体将ZNRD1的真核表达载体及其空载体转染胃癌细胞（AGS、SGC7901、MKN28）和小鼠成纤维细胞（NIH3T3），G418筛选后进行鉴定；5）利用脂质体将ZNRD1的小干扰RNA载体及其空载体转染药敏胃癌细胞（SGC7901）、正常胃组织上皮细胞（GES-1）、对长春新碱耐药的胃癌细胞（SGC7901/VCR）、药敏白血病细胞（HL-60）、对长春新碱耐药的白血病细胞（HL-60/VCR），G418筛选后进行鉴定；6）利用MTT实验检测ZNRD1高/低表达对细胞（AGS、SGC7901、MKN28、NIH3T3、GES-1）生长的影响；7）通过软琼脂克隆形成实验检测上调ZNRD1对AGS、MKN28细胞克隆形成能力的影响；8）通过裸鼠成瘤实验检测上调ZNRD1对AGS、MKN28细胞体内成瘤性的影响；9）通过流式细胞仪分析ZNRD1高/低表达对细胞（AGS、MKN28、NIH3T3、GES-1）的细胞周期的影响；10）通过流式细胞仪分析上调ZNRD1对细胞（AGS、MKN28、NIH3T3）的凋亡的影响；11）通过MTT实验检测ZNRD1高/低表达对细胞（SGC7901、SGC7901/VCR、HL-60、HL-60/VCR）体外药物敏感性的影响；12）通过肾包膜下移植法检测ZNRD1高/低表达对细胞（SGC7901、SGC7901/VCR）体内药物敏感性的影响；13）通过流式细胞仪分析ZNRD1高/低表达对细胞（SGC7901、SGC7901/VCR、HL-60、HL-60/VCR）内阿霉素蓄积和泵出的影响；14）通过透射电镜检测上调ZNRD1对SGC7901细胞凋亡敏感性的影响；15）通过流式细胞仪和DNA梯度试验检测ZNRD1高/低表达对细胞（SGC7901、SGC7901/VCR、HL-60）凋亡敏感性的影响；16）通过基因芯片检测ZNRD1高/低表达对胃癌细胞内基因表达谱的影响；17）利用RT-PCR、Western blot对基因芯片的结果进行鉴定；18）利用报告基因实验检测ZNRD1对cyclin D1的启动子活性的调节作用；19）利用报告基因实验检测ZNRD1高/低表达对MDR1的启动子活性的调节作用；20）利用激酶试验检测ZNRD1对cyclin E-CDK2 激酶活力的影响；21）利用反义核酸技术抑制p21的表达；通过流式细胞仪检测抑制p21对ZNRD1介导的细胞周期阻滞的影响；22）利用反义核酸技术抑制p27的表达；通过流式细胞仪检测抑制p27对ZNRD1介导的细胞周期阻滞的影响；23）利用脂质体转染法上调Skp2的表达；通过流式细胞仪检测上调Skp2对ZNRD1介导的细胞周期阻滞的影响；24）利用Western blot检测ZNRD1对p27和Skp2的蛋白稳定性的影响；25）利用微血管密度实验检测ZNRD1对AGS、MKN28细胞裸鼠移植瘤微血管形成的影响；26）利用ELISA检测ZNRD1对AGS、MKN28细胞培养上清和移植瘤匀浆中VEGF165含量的影响；27）利用脂质体转染法、MTT实验、肾包膜下移植法、流式细胞仪和DNA梯度试验检测新耐药相关分子DARPP-32对细胞（SGC7901、SGC7901/VCR、对阿霉素耐药的胃癌细胞SGC7901/ADR）多药耐药表型的影响；利用脂质体转染法和MTT实验检测下调ZNRD1对DARPP-32介导的胃癌多药耐药的调控作用。

When the aphids were feeding on Ww2730 seedlings, the time to 1st probing was delayed compared to those on Xiaoyan22 and Batis, searching was interrupted before 1st probing, and 1st probing was shorter than that on Xiaoyan22 and Batis significantly. Duration of pd Ⅱ-1 on Ww2730 and Xiaoyan22 was much longer than that on Batis. Duration of pd in C waveforms on Ww2730 was longer than that on Xiaoyan22 and Batis remarkedly. Times and duration of G waveform on Ww2730 were more and longer. Times of spot G waveform on Batis were more than on Ww2730 significantly, but the duration was shorter than those on Xiaoyan22 and Ww2730. Total time of E1 waveform, max longest duration of E1 fractions and mean duration time of E1 fractions following E2 wavefrom on Xiaoyan22 and Batis were all longer than on Ww2730 statistically. There were no differenences among mean durations of 1st E1 wavefrom on three varieties, but mean durations of other E1 waveforms on Ww2730 and Xiaoyan22 were shorter than those on Batis. The other waveform parameters, include times and durations of F and E2 were all not different on three wheat varieties.It is suggested that the resistance mechanism of wheat variety Ww2730 to S.

麦长管蚜在品种Ww2730上口针开始接触叶面时间显著晚于小偃22和Batis，第1次持续刺探前的刺探次数显著多于小偃22和Batis，开始刺入叶面后第1次刺探的持续时间极显著短于小偃22和Batis；在Ww2730和小偃22上的PdⅡ-1的平均时间显著长于Batis；Ww2730上C波阶段2个Pd波间的间隔平均时间显著大于另外2个品种；在Ww2730木质部取食的次数和时间显著大于其它2个品种；Batis上的点G波的次数极显著地高于Ww2730，而平均持续时间极显著短于小偃22和Ww2730；在Batis和小偃22上E1波的总时间，E1波的最大值、跟随E2波的E1波的平均时间均显著大于Ww2730；3个品种间第1次E1波持续的时间差异不显著，但Ww2730和小偃22上其它E1波持续的时间极显著少于Batis；3个品种上其它波形参数，包括F波的次数和总时间、E2波的次数和总时间没有差异。

Beyonce knowles唱的啊 You need to stop playing round with all them clowns and the wangstas Good girls gotta get down with the gangstas Go head girl put some back and some neck up on it While I stand up in the background and check up on it Ooh Boy you looking like you what you see Won't you come over check up on it,(I'm gonna let you work up on it) Ladies let em check up on it,(watch it while he check up on it) Dip it, pop it, twirk it, stop it, check on me tonight If you got it, flaunt it, boy I know you want it While I turn around you watch me check up on it Oohhh you watchin me shake it, I see it in ya face 'n' Ya can't take it, it's blazin, you watch me in amazement You can look at it, as long as you don't grab it If you don't go braggin, I'm a let you have it You think that I am teasin, but I ain't got no reason I'm sure that I can please ya, but first I gotta read ya Ooh Boy you looking like you like what you see Won't you come over check up on it (I'm gonna let you work up on it) Ladies let em check up on it (watch it while he check up on it) Dip it, pop it, twirk it, stop it, check on me tonight (x2) I can tell you wanna taste it, but I'm gone make you chase it Got to be patient, I like my men patient More patience, you take might get you in more places You can't be abrasive, has to know to pace it If I let you get up on it, you gotta make a promise That you gon put it on me, like no ones put it on me Don't bore me, just show me, all men talk but don't please I can be a tease, but I really wanna please you Ooh Boy you lookin like you like what you see Won't you come over check up on it,(I'm gonna let you work up on it) Ladies let em check up on it,(watch it while he check up on it) Dip it, pop it, twirk it, stop it, check on me tonight (x2) I'm checking on you boo, do what chu do And while dance I'ma glance at this beautiful view I'm keep my hands in my pants, I need to glue em w/ glue I'm in a trance all eyes on you and your crew Me and my mans don't dance, but to feel ya'll bump and grind If won't hurt if you gone try one time They all hot, but let me see this ones mine Its slim thug and DC outta H town Ohh Boy you looking like you like what you see Won't you come over check up on it,(I'm gonna let you work up on it) Ladies let em check up on it,(watch it while he check up on it) Dip it, pop it, twirk it, stop it, check on me tonight x4

slimthug，你必须停止玩回合与所有这些小丑与wangstas好女孩gottagetdown 随着gangstas走头女孩把一些背部和颈部一些比它在我站起背景及检查它oohboy你看你喜欢什么，你会看到你不来对清理出来，(我gonna让你工作起来就)让各位em察看着它，沾，流行资讯，twirk它，阻止它， checkonme今晚如果你有它，炫耀它，男孩我知道你要它当我转身，你看我检查它oohhh你watchin 我摇，我看它在亚面子'n'ya不能把它，它的blazin，你看我一无所有你可以看看，只要你不抓这个问题，如果你不去braggin，我是一个让你如果你觉得我teasin，但我是不会有任何理由，我相信我能请娅但是，我首先gotta阅读娅ooh男孩找你喜欢你喜欢你见到不会你来超过清理它(我gonna让你工作起来就)让各位em检查它沾，流行资讯，twirk它，阻止它， checkonmetonight(x2)，我可以告诉你想品尝，但我已离开人世，使你追逐它必须有耐心，我喜欢我的男病人更耐心，你也许可以得到你的地方越来越多，你不能磨料已经知道它的速度，如果我让你起床，那 yougotta做出承诺，你gonputitonme，不一样的说得对我并不负有我，只告诉我，所有男人说话，但不讨人喜欢，我可以戏耍，但是我真的想请你oohboylookin你喜欢你喜欢你见到不会你来对清理出来，(我gonna让你工作起来就)让各位em察看着它，沾，流行资讯，twirk它，阻止它， checkonmetonight(x2)我查你boo，做什么不楚而舞i'ma一眼这个美丽的看法，我把我的手在我的裤子，我需要胶水emw/胶水我在发呆都看你和你的船员和我我mans不跳舞，但感觉ya'll碰及研磨如果不会伤害如果你去尝试一次他们都热，但让我看到这样的矿井其slimthug直流outtah镇ohhboy你看喜欢你喜欢你见到不会你来清理，那(我gonna让你工作起来就)让各位em察看着它，沾，流行资讯，twirk它，阻止它， checkonme今晚x4

Slim Thug You need to stop playing round with all them clowns and the wangstas Good girls gotta get down with the gangstas Go head girl put some back and some neck up on it While I stand up in the background and check up on it Ooh Boy you looking like you what you see Won't you come over check up on it,(I'm gonna let you work up on it) Ladies let em check up on it,(watch it while he check up on it) Dip it, pop it, twirk it, stop it, check on me tonight If you got it, flaunt it, boy I know you want it While I turn around you watch me check up on it Oohhh you watchin me shake it, I see it in ya face 'n' Ya can't take it, it's blazin, you watch me in amazement You can look at it, as long as you don't grab it If you don't go braggin, I'm a let you have it You think that I am teasin, but I ain't got no reason I'm sure that I can please ya, but first I gotta read ya Ooh Boy you looking like you like what you see Won't you come over check up on it (I'm gonna let you work up on it) Ladies let em check up on it (watch it while he check up on it) Dip it, pop it, twirk it, stop it, check on me tonight (x2) I can tell you wanna taste it, but I'm gone make you chase it Got to be patient, I like my men patient More patience, you take might get you in more places You can't be abrasive, has to know to pace it If I let you get up on it, you gotta make a promise That you gon put it on me, like no ones put it on me Don't bore me, just show me, all men talk but don't please I can be a tease, but I really wanna please you Ooh Boy you lookin like you like what you see Won't you come over check up on it,(I'm gonna let you work up on it) Ladies let em check up on it,(watch it while he check up on it) Dip it, pop it, twirk it, stop it, check on me tonight (x2) I'm checking on you boo, do what chu do And while dance I'ma glance at this beautiful view I'm keep my hands in my pants, I need to glue em w/ glue I'm in a trance all eyes on you and your crew Me and my mans don't dance, but to feel ya'll bump and grind If won't hurt if you gone try one time They all hot, but let me see this ones mine Its slim thug and DC outta H town Ohh Boy you looking like you like what you see Won't you come over check up on it,(I'm gonna let you work up on it) Ladies let em check up on it,(watch it while he check up on it) Dip it, pop it, twirk it, stop it, check on me tonight x4

slimthug，你必须停止玩回合与所有这些小丑与wangstas好女孩gottagetdown 随着gangstas走头女孩把一些背部和颈部一些比它在我站起背景及检查它oohboy你看你喜欢什么，你会看到你不来对清理出来，(我gonna让你工作起来就)让各位em察看着它，沾，流行资讯，twirk它，阻止它， checkonme今晚如果你有它，炫耀它，男孩我知道你要它当我转身，你看我检查它oohhh你watchin 我摇，我看它在亚面子'n'ya不能把它，它的blazin，你看我一无所有你可以看看，只要你不抓这个问题，如果你不去braggin，我是一个让你如果你觉得我teasin，但我是不会有任何理由，我相信我能请娅但是，我首先gotta阅读娅ooh男孩找你喜欢你喜欢你见到不会你来超过清理它(我gonna让你工作起来就)让各位em检查它沾，流行资讯，twirk它，阻止它， checkonmetonight(x2)，我可以告诉你想品尝，但我已离开人世，使你追逐它必须有耐心，我喜欢我的男病人更耐心，你也许可以得到你的地方越来越多，你不能磨料已经知道它的速度，如果我让你起床，那 yougotta做出承诺，你gonputitonme，不一样的说得对我并不负有我，只告诉我，所有男人说话，但不讨人喜欢，我可以戏耍，但是我真的想请你oohboylookin你喜欢你喜欢你见到不会你来对清理出来，(我gonna让你工作起来就)让各位em察看着它，沾，流行资讯，twirk它，阻止它， checkonmetonight(x2)我查你boo，做什么不楚而舞i'ma一眼这个美丽的看法，我把我的手在我的裤子，我需要胶水emw/胶水我在发呆都看你和你的船员和我我mans不跳舞，但感觉ya'll碰及研磨如果不会伤害如果你去尝试一次他们都热，但让我看到这样的矿井其slimthug直流outtah镇ohhboy你看喜欢你喜欢你见到不会你来清理，那(我gonna让你工作起来就)让各位em察看着它，沾，流行资讯，twirk它，阻止它， checkonme今晚x4

Stratford-on-Avon, as we all know, has only one industry-William Shakespeare-but there are two distinctly separate and increasingly hostile branches.

众所周知Stratford-on-Avon只有一个产业，那就是威廉莎士比亚。但这儿却有两个相互独立的分支，他们之间日益变得对立。

Text 2 Stratford-on-Avon, as we all know, has only one industry-William Shakespeare-but there are two distinctly separate and increasingly hostile branches.

众所周知 Stratford-on-Avon 只有一个特色，那就是威廉 o 莎士比亚，但这儿却有两个相互独立的部门，他们随着时间的变化而日益变得敌对。

I lived in Stratford-on-Avon until about five years ago, in fact we moved from Stratford to Los Angeles, so you can imagine what a seamless transition this was.

我过去一直住在埃冯河畔斯特拉特福德（ Stratford-on-Avon ），直到大约五年前才从那搬到了洛杉矶，你可以想象出这两地的环境是多么地类似。

Last week Royal Doulton 11, the largest employer in Stoke-on-Trent 12, announced that it is eliminating 1,000 jobs—one-fifth of its total workforce.

上星期，特伦特河畔斯托克（Stoke-on-Trent）地区最大的雇主皇家道尔顿宣布它将裁减1000个工作岗位，即全公司五分之一的员工。

The Fearnley-Whittingstalls married in July 1962 in Henley-on-Thames, where Jane's parents had settled after returning from what was then Rhodesia.

Fearnley-Whittingstalls 于1962年7月在Henley-on-Thames结婚，这也是她父母从罗德尼亚返回后定居的地方。

Jim Horney, a former Democratic congressional staffer now at the liberal Centre on Budget and Policy Priorities, a think-tank, says it has been made even harder by the disappearance of any culture of restraint in Congress.

吉姆·霍尼以前曾在民主党国会中任职，现在他受聘于一家自由派研究机构：预算与政策优先权中心（Centre on Budget and Policy Priorities）。他认为国会中遏制传统的缺失使得对政府政策的监管变得更为困难。

However, as the name（read－only memory）implies, CD disks cannot be written onorchanged in any way.

然而，正如其名字所指出的那样，CD盘不能写，也不能用任何方式改变其内容。

Galvanizes steel pallet is mainly export which suits standard packing of European Union, the North America. galvanizes steel pallet is suitable to heavy rack. Pallet surface can design plate type, corrugated and the gap form, satisfies the different requirements.

镀锌钢托盘多用于出口，替代木托盘，免薰蒸，符合欧盟、北美各国对出口货物包装材料的法令要求；喷涂钢托盘适用于重载上货架之用，托盘表面根据需要制作成平板状、波纹状及间隔形式，满足不同的使用要求。