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For quantitatie real-time PCR of DR4 and DR5 transcripts, triplicated samples containing 9 l of cDNA with 10 l of Taqman Uniersal PCR Master Mix (Applied Biosystems, Foster City, CA) and 1 l of 20xAssays-on-demand Gene Expression Product (DR4; Hs 00269492_m1, DR5; Hs 00366272_m1, Applied Biosystems) were pre-incubated at 50oC for 2 minutes and subsequently at 95oC for 10 minutes.

对于进行定量实时PCR的DR4 和 DR5转录产物，使用10L 美国应用生物系统公司生产的 Taqman Uniersal PCR Master Mix试剂，1L的20xAssays-on-demand Gene Expression Product (美国应用生物系统公司生产的DR4； Hs 00269492_m1， DR5； Hs 00366272_m1)，先将其在50度下孵化2分钟，然后在95度下孵化10分钟，用这两种试剂将含有9L cDNA的样品扩增两倍。

For example, capital regulations and provisioning rules as agreed by the Basel committee on banking supervision, and industry governance structures, especially in the area of risk management, need to restrain excessive risk-taking in upturns and discourage excessive conservatism when credit to companies and households is most needed.

例如，巴塞尔银行监管委员会(Basel Committee on Banking Supervision)制定的资本要求和供应规定，以及行业管理结构，需要遏制上升时期的过度冒险行为，劝阻在企业和家庭最需要信贷时的过度保守主义。

From what I can tell the on/off switch is actually a very flimsy spring that vibrates with the slightest movement.

从什么我可以告诉On / Off开关，其实是一个非常脆弱的春天震动与有丝毫的运动。

Dongpinghu Reservoir is located in the congregation of Dongping, Liangshan and Wenshang of Shangdong province or connected section of withed part to narrow part in yellow river down reach. The reservoir was built in 1958, it was expanded on the basis of the natural like. The reservoir consists of bounded bank, secondary lake bank, segregate intake gate. The perimeter of bank is 88. 3km, and it was constructed in later 1950's.

中文题名东平湖水库围坝东段渗流控制研究副题名外文题名 Study on controlling permeability of east dam in Dongpinghu reservoir 论文作者余强导师佴磊教授学科专业地质工程研究领域\研究方向工程地质学位级别博士学位授予单位吉林大学学位授予日期2002 论文页码总数97页关键词水库围坝渗流控制防渗处理馆藏号BSLW /2003 /TV697 /1 东平湖水库座落在山东省东平、梁山、汶上三个县的交界处，位于黄河下游宽河道与窄河道的过渡段上。

He just yammers on about nothing.

&on&在这里加深了唠叨的程度。

One of them was designed as a potential fluorescent probe to detect aldehyde group reductase in vivo, with a disctinct wavelength movement between the on-off states.

我们对其中一个分子进行结构修饰后，发展了一种基于ICT效应的荧光比率型荧光探针，它的on-off状态之间的波长变化达到了26 nm，今后有望用于体内醛基还原酶的检测。

Has not launched a four-year Korean works of Song, Hyun-bin cooperation with the recent performances "They live in a world",despite the new play in two of his desperately Chuangxi, engage in intimate, but there has been "box office poison" of thetitle of the two , The notorious yet having the wash,"They live in a world," the ratings not only in last place, with thenews story "On air" high similarity, are the subject of ridicule in the entertainment business around the dark inside, butboth The ratings can be said to be in far worse days.

已四年没有推出韩剧作品的宋慧乔，近来与玄彬合作演出《他们生活的世界》，尽管在新戏中两人拼命演床戏、搞亲热，但曾经都有&票房毒药&称号的两人，这次仍然无缘洗刷恶名，《他们生活的世界》收视率不仅敬陪末座，更传出剧情与《On air》相似度高，主题都是围绕在嘲讽娱乐圈的黑暗内幕，但两者收视率却可说是天差地远。

This vocal mic provides high quality sound reproduction, durable die-cast construction, and best of all, a convenient, built-in On/Off switch.

这声音，麦克风提供高品质的声音复制，持久压铸建设，最重要的是，方便，内置的On / Off开关。

The most jaw-dropping moment of the show was when Spears performed Breath On Me accompanied by two aerialists, who turned their bodies into a bed and sent Britney flying into the sky with them!

最让人吃惊的是当Britney表演Breathe On me的时候，2个空中特技人员把身体变成了一张床，然后把Britney和自己一起送到空中，不仅如此，她在空中毫无畏惧，还做了些惊人的动作！

In the work, Spin-on-dopants, as dopants, thermally diffused in the rapid thermal processor with different dwell time, temperatures, and ambients.

实验中使用液态旋涂磷源(Spin-on-dopants，SOD)做为掺杂剂，利用RTP技术在不同时间、温度和气氛下进行了快速热扩散。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。