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Methods The clpE-deficient strain was constructed by LFH-PCR and identified by PCR and sequencing. The impact of clpE mutant on the virulence of S. pneumoniae was evaluated in a mouse model. In addition, we also studied the effect of clpE mutant on adherence and invasion of host cells. Real time RT-PCR was used to measure the mRNA expression levels of autolysin A, pneumococcal surface adhesion A, pneumolysin, pneumococcal surface protein A and neuraminidase The clpE gene was replaced completely by erm cassette.

用长臂同源多聚酶链式反应方法失活clpE基因,用PCR、测序鉴定缺失菌株,通过动物实验观察clpE基因缺失株毒力改变情况,同时用细胞实验比较clpE基因缺失株和野生菌对宿主细胞的粘附和侵袭能力,最后用实时荧光定量PCR分析自溶素(major autolysin A,lytA)、表面黏附素A(pneumococcal surface adhesion A,psaA)、溶血素(pneumolysin,ply)、肺炎球菌表面蛋白A(pneumococcal surface protein A, pspA和神经氨酸酶(neuraminidase, nanA)的表达。

Particular configurations on bedding planes and on the surface perpendicular to the bedding planes lead to different explanations for the genesis of these silty dykes:(1) they are considered as the oldest trace fossils of metazoan according to the spindle-crack of siltstones on the bedding plane and the sand-filling tube configurations on the surface perpendicular to the bedding plane;(2) they are considered as the liquefied sand-veins induced by earthquake according to the sinuous configuration of siltstone dykes on the surface perpendicular to the bedding plane and the obvious liquation feature.

由于该粉砂岩岩墙在层面上和层内具有特别的形态,因而对其成因具有多种解释:在层面上呈纺锤状粉砂岩裂缝以及在层内呈砂质充填管形态,从而被认为是最古老的后生动物遗迹化石;在层内呈弯弯曲曲的粉砂岩岩墙以及明显的流体化作用特点,所以又被解释为地震振荡作用形成的液化砂岩脉。

The research on bound rubber and reinforcing effect of mineral filler showed that there are four kind of interactions between filler and rubber matrix in uncured mix The unbounded rubber on the surface of filler will solve in organic solvent The rubber bounded by stronger physical or chemical interaction with filler will dissolve in organic solvent The bound rubber on the surface of filler by different extent acid-base interactions will solve in basic organic solvent The bound rubber on the surface of filler by chemical action (no acid-base type) will dissolve in basic organic solvent

结合橡胶和增强作用的研究表明,在混炼胶中,硅橡胶与矿物填料的作用形式有四种。没有联结在矿物表面的非结合橡胶和仅靠物理吸附联结在填料表面的硅橡胶将溶解于有机溶剂;与填料有较强相互作用的硅橡胶不溶于有机溶剂;通过不同层次的酸碱作用联接在矿物表面的硅橡胶将溶解于碱性有机溶剂;与填料有牢固化学结合的硅橡胶不溶于碱性有机溶剂。

It is the fluorosilicic ion that exsits in the slurry and plays an important role in flotation, fluorosilicic ion not only reacted with Fe〓 on the surface of aegirine to form ionic compound, but also formed stable Si-F bond in Si-O etching reaction. Only ionic compound was obtained when it was attached to hematite surface according to the theories of coordination chemistry, Compared with SiF〓, Fatty acid ion preferentially acts with Fe〓 on the minerals surface, therefore, SiF〓 Can't obstruct the adsorption of collector on the surface of hematite completly, but can reduce the flotation recovery of aegirine selectively.

运用FTIR、XPS等测试方法仔细研究了氟硅酸盐抑制剂对含铁硅酸盐钠辉石的选择性抑制作用机理,氟硅酸盐在弱酸性条件下对赤铁矿浮选的抑制作用很微弱,而对含铁硅酸盐钠辉石强烈抑制,从而扩大了两者之间的浮选差异;SiF〓是矿浆中起抑制作用的主要成分,SiF〓不仅与钠辉石表面的Fe〓形成离子型配合物,而且还与Si-O键形成稳定的Si-F共价键,而与赤铁矿表面只能形成离子型配合物,根据晶体配位场理论,与SiF〓相比较羧酸根离子将优先与矿物表面Fe〓发生配位作用,因此SiF〓的存在并不能完全阻碍捕收剂在赤铁矿表面的吸附作用,却能够选择性地抑制钠辉石的上浮。

On the basis of previously research, this paper re-probe into the mechanism of electro-chemist mechanical manufacturing process, making the rational technological parameter, and developing the manufacturing equipment. Through a lot of technology tests, get a lot of labratory data, accordingly, researched the surface feature of the electro-chemist manufacturing process, and deeply discussed its influence on the work piece feature. The last result is that: the electro-chemist mechanical manufacturing process can reduce the low-frequency component significantly, but on high-frequency component, it has little influence, having obviously positive pole flat effect. After manufacture, the tip parts on the surface of the work piece is cut off, there is micro "high land" shape, the parameter for surface roughness is reduced a lot, the surface outline of the work piece is negative, the bearing length rate is cliffy. Also the surface quality of the work piece is improved. This can help to avoid changing the shape, improve the feature of rub, gear and reduce the time to suit. This would also ensure the precision and result in a longer work piece life.

在以前研究的基础上,本论文重新探讨了电化学机械复合光整加工工艺的机理,确定了较合理的工艺参数,对电化学机械复合光整加工设备进行了改进,通过工艺实验广泛地搜集了大量实验数据,依据实验数据,多角度地研究了电化学机械复合光整加工的表面形貌特点,并且从理论上深入讨论了电化学机械复合光整加工表面形貌对工件工作性能的影响,最后得出的结论为:电化学机械复合光整加工对工件原始表面的低频成分有较大程度的降低,而对高频成分影响较小,有明显的阳极整平作用,被加工工件表面的尖峰状凸起被去除,工件表面呈现出微观&高原型&,表面轮廓不平度高度特性参数大幅度降低,工件表面轮廓分布集中,且呈负偏态,轮廓偏斜度为负值,轮廓支承长度率曲线陡峭,工件的表面质量得到综合改善,这对提高零件的抗接触变形能力、改善摩擦、磨损性能、降低工件的磨合时间、提高工件的精度保持性、延长工件的寿命均有利。

Abstract] Objective To Treating the nano-SiO2 particle by silicon coupling reagent, functionalize the SiO2 by making double bond on its surface. Polymerizing on the surface of nano-SiO2 surface by the method called emulsion polymerization, the nano-SiO2 is covered by polymer to get the PMMA-KH-570-nano-SiO2 complex material. Test the surface structure of the surface of nano-SiO2 by FTIR, and test sort of its mechanical performances.Comparing the treated nano-SiO2 which is directly dispended in the PMMA emulsion with the SiO2 without any treatment, we are trying to find an effective method which can not only increase the dispersion of SiO2, but also improve the mechanical performance of the artificial teeth.

目的 通过硅烷偶联剂KH-570处理SiO2纳米粒子,向其表面引入双键使其功能化,采用乳液聚合的方法在SiO2纳米粒子表面进行接枝聚合,实现高分子对SiO2纳米粒子的表面包覆处理,制得PMMA-KH-570-SiO2纳米复合材料,采用FTIR对SiO2纳米粒子表面结构进行了表征研究,并且测定了它的多种机械性能,与表面处理过的SiO2纳米粒子直接分散至PMMA乳液中制得的复合材料、未添加任何增强物质的空白组进行比较,探索一种能够提高SiO2在基体中的分散性,为义齿复合材料的研究和开发提供理论依据和参考。

Three part of research works had been carried out in the dissertation. The first part: a kind of multidentate amine modified silica gel was synthesized through the following steps:(1) introduce the amino groups onto the surface of silica-gel by the treatment of surface silanol groups withγ-aminopropyl trimethoxy silane;(2) michael addition of excess trihydroxymethyl propyl triacrylate to amino groups on the silica gel surface;(3) amidation of the unreacted double bonds of TMPTA on the surface of TMPTA modified silica gel with diethylenetriamine;(4) the multidentate amine modified silica gel was finally obtained by the reaction of amino groups on the surface of DETA modified silica gel with double bond of butyl acrylate.

本论文进行了三部分研究工作,第一部分:通过多步反应对硅胶进行改性合成了一种多齿胺改性硅胶配体,合成过程如下:1通过γ-氨丙基三甲氧基硅烷与硅胶表面的硅羟基反应,使硅胶表面带有氨基;2利用硅胶表面氨基与过量的三羟甲基丙烷三丙烯酸酯发生Michael加成反应;3利用二乙烯三胺与TMPTA改性硅胶表面未反应的双键发生反应使硅胶表面氨基化;4最后用丙烯酸正丁酯与DETA改性硅胶表面氨基充分反应成功合多齿胺改性硅胶配体。

The invention discloses an electrochemical method for preparing a hydroxylapatite particle thin coating on the surface of a metal implant, which comprises the following steps: electrolyte is put into a container equipped with a constant temperature heating system and heated to be 30 DEG C to 95 DEG C, and then the temperature is kept; platinum is taken as the anode and evenly distributed around the container, and the metal implant is taken as the cathode, put in the middle of the container and totally soaked in the electrolyte; DC voltage of 2V to 4V is added between the anode and the cathode, the surface of the metal implant has cathode reduction reaction, the pH value is increased and the supersaturation degree of the hydroxylapatite is also increased, so as to crystalize on the surface of titanium; after 0.5h to 5h of deposition, a layer of hydroxylapatite particle thin coating which can be excellently adhered to the matrix metal is formed on the surface of the implant.

本发明公开了一种在金属植入体表面制备羟基磷灰石颗粒薄涂层的电化学方法,在装备了恒温加热系统的容器中装入电解液,并将电解液加热到30~95℃恒温;将铂作为阳极均匀分布在容器四周,金属植入体作为阴极置于容器中间,完全浸没在电解液中,两极之间加直流电压2~4V,金属植入体表面发生阴极还原反应,pH值升高,羟基磷灰石过饱和度增加,从而结晶在钛金属表面;经过0.5~5小时沉积后,在植入体表面即可形成一层与基体金属结合优良的、薄的羟基磷灰石颗粒薄涂层。

The weight percentage for the components of the hyaline coating is as follows: SiO2 45 to 54 percent, B2O3 15 to 20 percent, one or more of Na2O, K2O and Li2O, 10 to 24 percent, one or more of TiO2, ZrO2 and ZnO, 6 to 18 percent , and the rest is one or more of CaO, MgO and CeO2. Firstly, the glass powder is mixed with the mill additions to be pulped and then plated on the surface of titanium or the titanium alloy. After being dried, the surface of titanium or the titanium alloy is directly used for a thermal hydrogen treatment or smelted at a temperature of 900 to 1, 000 DEG C for 15 to 30 minutes and then a hyaline coating with a thickness of 20 to 70 micrometers is formed on the surface of titanium or the titanium alloy; secondly, titanium or the titanium alloy plated with the coating is conducted a thermal hydrogen treatment. After the treatment technique is finished, the coating flakes off automatically in the process of cooling; and finally, a surface treatment is supplemented to meet the requirement of the workpiece surface.

该玻璃质涂层成分按重量百分比:45-54SiO2,15-20B2O3,10-24Na2O、K2O、Li2O中的一种或多种,6-18TiO2、ZrO2、ZnO中的一种或多种,余量CaO、MgO、CeO2一种或多种,将玻璃粉与磨加物混合制浆并涂覆在钛或钛合金表面,干燥后直接用于热氢处理,或在900-1000℃下熔烧15-30min,在钛或钛合金表面制得20-70微米玻璃质涂层,随后将制有该涂层的钛或钛合金置于氢气气氛下进行热氢处理,处理工艺结束后,涂层在冷却过程中自剥落,最后辅以表面处理即可满足工件表面要求。

The result showed that the surface architecture and ultrastructure of I.punctatus ' gill and secondary gill lamellae were similar to those of other teleosts. The surface of gill arches and gill rakers was covered with pavement cells which were characterized by circular microridges, and secreting-holes were found among cells on the surface of gill arches. The base part of gill rakers were bigger in terms of diameter. The pavement cells' surface of gill filaments' middle part was characterized by microridges with fine lateral protuberances on both sides which were either fused or interruped, while the microridges disappeared on the pavement cells' surface on both top and base parts of gill filaments, forming dense granulated protuberances. The height and interlamellar space of secondary gill lamellae in I.punctatus were relatively higher and bigger, respectively.

结果表明,斑点叉尾鮰鳃的表面结构和微细结构与其他硬骨鱼类基本相似,鳃弓和鳃耙表面被具环形微嵴的扁平上皮细胞所覆盖,鳃弓表面细胞之间有孔洞;鳃耙基部较粗壮;中部鳃丝上皮细胞表面的微嵴两侧有细小的横突,有些微嵴出现融合或间断;端部和基部鳃丝上皮细胞表面的微嵴消失,形成密集颗粒状突起;鳃小片的高度和片间距较大。

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On The Surface
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According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm~150rpm时取得最大值,此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世,形体几无变化。

When I was in school, the rabbi explained everythingin the Bible two different ways.

当我上学的时候,老师解释《圣经》用两种不同的方法。