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Methods The clpE-deficient strain was constructed by LFH-PCR and identified by PCR and sequencing. The impact of clpE mutant on the virulence of S. pneumoniae was evaluated in a mouse model. In addition, we also studied the effect of clpE mutant on adherence and invasion of host cells. Real time RT-PCR was used to measure the mRNA expression levels of autolysin A, pneumococcal surface adhesion A, pneumolysin, pneumococcal surface protein A and neuraminidase The clpE gene was replaced completely by erm cassette.

用长臂同源多聚酶链式反应方法失活clpE基因,用PCR、测序鉴定缺失菌株,通过动物实验观察clpE基因缺失株毒力改变情况,同时用细胞实验比较clpE基因缺失株和野生菌对宿主细胞的粘附和侵袭能力,最后用实时荧光定量PCR分析自溶素(major autolysin A,lytA)、表面黏附素A(pneumococcal surface adhesion A,psaA)、溶血素(pneumolysin,ply)、肺炎球菌表面蛋白A(pneumococcal surface protein A, pspA和神经氨酸酶(neuraminidase, nanA)的表达。

Abundance of aluminium atom on the surface of diaspore is 1.7 times larger than that on the surface of kaolinite.Absorbing or ionizing H~+ result in charging on the surface of diaspore; Zata-potential of diaspore is variable with pH in the solution.

高岭石的荷电机理有两种:端面为氢离子的吸附和电离,电位随溶液pH的增大而降低;层面为金属离子的晶格取代,荷负电,与溶液的pH无关。

RESULTS: Both resting B cells and T cells did not express PD-L1 on their surface, however PD-L1 expression was significantly up-regulated on the surface of the activated B cells after 6 h stimulation with LPS or pokeweed mitogen, and the percentages of B cells that expressed PD-L1 reached a plateau at 24 h, which were (46.26±10.71)% with LPS and (43.67±6.14)% with PWM stimulation, respectively. No markedly change of PD-L1 expression on the surface of the activated T cells after stimulation with LPS was observed, but upregulation of PD-L1 expression was observed when stimulation with PWM.

结果: 静息B细胞和T细胞表面不表达PD-L1,LPS和PWM刺激6 h后表达PD-L1的B细胞百分率均显著高于静息B细胞,24 h达到最高,分别为(46.26±10.71)%和(43.67±6.14)%,之后随时间延长而降低;表达PD-L1的T细胞百分率在LPS刺激下无显著变化,但PWM刺激6 h后百分率明显高于静息条件,24 h达到最高,为(25.42±9.23)%,之后下降。

The research on bound rubber and reinforcing effect of mineral filler showed that there are four kind of interactions between filler and rubber matrix in uncured mix The unbounded rubber on the surface of filler will solve in organic solvent The rubber bounded by stronger physical or chemical interaction with filler will dissolve in organic solvent The bound rubber on the surface of filler by different extent acid-base interactions will solve in basic organic solvent The bound rubber on the surface of filler by chemical action (no acid-base type) will dissolve in basic organic solvent

结合橡胶和增强作用的研究表明,在混炼胶中,硅橡胶与矿物填料的作用形式有四种。没有联结在矿物表面的非结合橡胶和仅靠物理吸附联结在填料表面的硅橡胶将溶解于有机溶剂;与填料有较强相互作用的硅橡胶不溶于有机溶剂;通过不同层次的酸碱作用联接在矿物表面的硅橡胶将溶解于碱性有机溶剂;与填料有牢固化学结合的硅橡胶不溶于碱性有机溶剂。

The latest progress of the dynamic phenomenon on solid surfaces is summarized,including decay phenomenon of adatom islands, step fluctuations on the surface of metals,the hopping of special clusters on the surface of semiconductors,non-linear oscillation phenomenon on the surface of metal,etc.

总结了近年来国内外固体表面动态现象研究的最新进展,其中包括金属和半导体表面原子岛的塌落现象、金属和半导体表面台阶波动、半导体表面特异集团的跳跃、金属袁面非线性振荡现象及其它一些动态现象;描述了这些动态现象特征及形成机制,并展望了动态现象的研究方向和应用前景。

At 4 weeks following implantation, cells on the surface of coralline hydroxyapatite were found and connective tissues were seen in the material pores in the experimental group. Cells on the coralline hydroxyapatite were observed only in the control group. At 8 weeks, new bone formation was detected on the surface of coralline hydroxyapatite; bony tissue deposition and a few chondroid tissues were found in the pores or surrounding the pores in the experimental group. A few fibrous connective tissues were observed in the control group. At 12 weeks, abundant mature woven bone was detected on the surface of coralline hydroxyapatite; medullary cavity-like structure and vessels were found in some regions in the experimental group. No new bone or bony tissues were found in the control group.

植入材料后4周,实验组可见珊瑚羟基磷灰石表面有细胞生长,孔隙内有结缔组织长入;对照组仅见珊瑚羟基磷灰石表面有细胞生长。8周时珊瑚羟基磷灰石表面有新生骨形成,孔隙内和孔隙边缘可见骨样组织沉积和少量软骨样组织形成;对照组仅见少量纤维结缔组织长入。12周时珊瑚羟基磷灰石材料表面有较多成熟编织骨形成,部分区域可见髓腔样结构形成,并有血管长入;对照组仍未见新骨及骨样组织形成。

Offsctdruckereien during the paper version of the chaetomium reactor, mainly the fault surface caused by osteofibrous epicare of loose paper, this phenomenon is primarily a business card printing and membership card making poor-quality paper, the fault surface is imprinted on the surface of the ink is painted on the phenomenon of burnedi the Cabinet had turned white, from lead to block, paper surface covered with blankets, serious every business card printing and membership card will need to make 1 000 in the cleaning, washing, plates and rubberand once, eliciting business card printing and membership card making quality problems, delays in the production schedule and time of delivery.

胶印历程洋表现纸不净堆版的障碍,次给是纸弛暗地纤维布局紧密引起的纸弛脱不净变成的,这种本体次给是制卡和会员卡制作质量较不对的纸弛,其障碍暗地为痕迹不朱色暗地为安纸屑似的斑白本体,纸不净零落引起印版、橡皮布暗地沾满纸不净,惨轻时每制卡和会员卡制作1000弛不离必给擦洗印版和橡皮一次,从而引起制卡和会员卡制作质量题纲,耽搁不入产进量和交货工夫。

The paper version offsctdruckereien epicare reactor during the paper version of the chaetomium reactor, mainly the fault surface caused by osteofibrous epicare of loose paper, this phenomenon is primarily a business card printing and membership card making poor-quality paper, the fault surface is imprinted on the surface of the ink is painted on the phenomenon of burnedi the Cabinet had turned white, from lead to block, paper surface covered with blankets, serious every business card printing and membership card will need to make 1 000 in the cleaning, washing, plates and rubberand once, eliciting business card printing and membership card making quality problems, delays in the production schedule and time of delivery.

一、纸弛脱不净堆版胶印历程洋表现纸不净堆版的障碍,次给是纸弛暗地纤维布局紧密引起的纸弛脱不净变成的,这种本体次给是制卡和会员卡制作质量较不对的纸弛,其障碍暗地为痕迹不朱色暗地为安纸屑似的斑白本体,纸不净零落引起印版、橡皮布暗地沾满纸不净,惨轻时每制卡和会员卡制作1000弛不离必给擦洗印版和橡皮一次,从而引起制卡和会员卡制作质量题纲,耽搁不入产进量和交货工夫。

The invention discloses an electrochemical method for preparing a hydroxylapatite particle thin coating on the surface of a metal implant, which comprises the following steps: electrolyte is put into a container equipped with a constant temperature heating system and heated to be 30 DEG C to 95 DEG C, and then the temperature is kept; platinum is taken as the anode and evenly distributed around the container, and the metal implant is taken as the cathode, put in the middle of the container and totally soaked in the electrolyte; DC voltage of 2V to 4V is added between the anode and the cathode, the surface of the metal implant has cathode reduction reaction, the pH value is increased and the supersaturation degree of the hydroxylapatite is also increased, so as to crystalize on the surface of titanium; after 0.5h to 5h of deposition, a layer of hydroxylapatite particle thin coating which can be excellently adhered to the matrix metal is formed on the surface of the implant.

本发明公开了一种在金属植入体表面制备羟基磷灰石颗粒薄涂层的电化学方法,在装备了恒温加热系统的容器中装入电解液,并将电解液加热到30~95℃恒温;将铂作为阳极均匀分布在容器四周,金属植入体作为阴极置于容器中间,完全浸没在电解液中,两极之间加直流电压2~4V,金属植入体表面发生阴极还原反应,pH值升高,羟基磷灰石过饱和度增加,从而结晶在钛金属表面;经过0.5~5小时沉积后,在植入体表面即可形成一层与基体金属结合优良的、薄的羟基磷灰石颗粒薄涂层。

The weight percentage for the components of the hyaline coating is as follows: SiO2 45 to 54 percent, B2O3 15 to 20 percent, one or more of Na2O, K2O and Li2O, 10 to 24 percent, one or more of TiO2, ZrO2 and ZnO, 6 to 18 percent , and the rest is one or more of CaO, MgO and CeO2. Firstly, the glass powder is mixed with the mill additions to be pulped and then plated on the surface of titanium or the titanium alloy. After being dried, the surface of titanium or the titanium alloy is directly used for a thermal hydrogen treatment or smelted at a temperature of 900 to 1, 000 DEG C for 15 to 30 minutes and then a hyaline coating with a thickness of 20 to 70 micrometers is formed on the surface of titanium or the titanium alloy; secondly, titanium or the titanium alloy plated with the coating is conducted a thermal hydrogen treatment. After the treatment technique is finished, the coating flakes off automatically in the process of cooling; and finally, a surface treatment is supplemented to meet the requirement of the workpiece surface.

该玻璃质涂层成分按重量百分比:45-54SiO2,15-20B2O3,10-24Na2O、K2O、Li2O中的一种或多种,6-18TiO2、ZrO2、ZnO中的一种或多种,余量CaO、MgO、CeO2一种或多种,将玻璃粉与磨加物混合制浆并涂覆在钛或钛合金表面,干燥后直接用于热氢处理,或在900-1000℃下熔烧15-30min,在钛或钛合金表面制得20-70微米玻璃质涂层,随后将制有该涂层的钛或钛合金置于氢气气氛下进行热氢处理,处理工艺结束后,涂层在冷却过程中自剥落,最后辅以表面处理即可满足工件表面要求。

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I am accused of being overreligious," she said in her quiet, frank manner,"but that does not prevent me thinking the children very cruel who obstinately commit such suicide.""

客人们在卡罗利娜·埃凯家里,举止就文雅一些,因为卡罗利娜的母亲治家很严厉。

Designed by French fashion house Herm è s, this elegant uniform was manufactured in our home, Hong Kong, and was the first without a hat.

由著名品牌 Herm è s 设计,这件高贵的制服是香港本土制造,是我们第一套不配帽子的制服。

Do not 'inflate' your achievements and/or qualifications or skills .

不要 '夸大' 你的业绩或成果,条件或者技能。