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We optimized reaction system for labelling-antibody in which the optimal amount of the purified anti-Stx2B IgG conjugated with colloidal gold beads was 60μg/mL, the purified antibody for E.coli O157 was 57μg/mL, the optimal pH was 8.2, the size of colloidal gold partical was 20nm, the optimization of stabilizing agent was BSA, the optimization buffer was boracic acid buffer with pH 8.2, the optimization of preserving fluid and eluant was boracic acid buffer with pH 8.2 including 5mM NaCl-1%BSA, confining liquid for NC membrane was 0.01% PBST with 3%BSA, the amount of polyclonal antibody against E.coli O157 and Stx2 conjugated with colloidal gold beads for conjugate pad was 3μg respectively, the amount of anti-Stx monoclonal antibody for test line was 0.1μg and 1μg for E.coli O157, the amount of goat anti rabbit IgG for control line of both GICA were 1μg.

测定了胶体金颗粒的最优化反应体系：胶体金颗粒的大小为20nm；抗体与胶体金溶液结合的最佳pH约为8.2；最佳蛋白结合量分别为抗大肠杆菌O157 IgG为57μg/mL，抗重组Stx2B IgG为60μg/mL；最佳稳定剂为BSA；最佳缓冲液为pH8.2硼酸溶液；最佳金标保存液和洗涤液为5mM NaCl-1%BSA的pH 8.2的硼酸缓冲液；NC膜的封闭液为3%BSA的0.01mol/L PBST；Stx2试纸条和大肠杆菌O157试纸条的质控线上的羊抗兔IgG的多克隆抗体最佳点样量均为1μg，其检测线的抗Stx2的单克隆抗体的点样量为0.1μg、抗大肠杆菌O157的单克隆抗体的点样量为1μg，结合垫上的金标抗大肠杆菌O157和重组Stx2B多克隆抗体点样量均为3μg。

40000 Production bottles / hour bottled beer production line and 9000-18000 cans / hour filling cans production line, product quality and service by the users.

生产24000-40000瓶/小时啤酒瓶装生产线和9000-18000罐/小时易拉罐灌装生产线，产品的质量与服务得到了用户的好评。

Thus, the return here affects not just the bottom-line of a company's operations, but also its top line.

因此，这种回报不仅影响了公司操作的最顶层，同时也影响到了公司的最底层。

boundary line of transaction curve ; parametric equation of coordination ; length of boundary line

本文导出了缓和曲线边线的坐标参数方程及边线长度

On the map, the boundary line is a line winding, but is not the case in the field.

在地图上，边界线确实是一条弯弯曲曲的线条，但是在实地并不是这样。

The boundary line between two provinces is shown by a silver line on the map.

这两个省的省界在地图上由银线标出。

Principle of design changing a line is: Optimize a line model, tortuous path is taken in, escape bourg, improve grade.

改线设计原则是：优化线型，弯道取在，避开村镇，提高等级。

The only exceptions being the Southern branch of line C and the outer sections of line D.

唯一的例外是线C 和线D 的外面部分南部的分支。

A main line to which branch line are connected.

连接有支线的线路。

Used in the CCTV system, the antenna television system branch line, subscriber line and other electronic devices.

本产品适用于闭路电视、共用天线电视系统作分支线和用户线及其它电子装置。

Article 144 The Government of the Hong Kong Special Administrative Region shall maintain the policy previously practised in Hong Kong in respect of subventions for non-governmental organizations in fields such as education, medicine and health, culture, art, recreation, sports, social welfare and social

第一百四十四条香港特别行政区政府保持原在香港实行的对教育、医疗卫生、文化、艺术、康乐、体育、社会福利、社会工作等方面的民间团体机构的资助政策。原在香港各资助机构任职的人员均可根据原有制度继续受聘。

Small wonder, then, that the Chinese spend more in the shop than any other group of foreign visitors do .

这样的小惊喜，使中国顾客比任何国家的人消费得更多。