查询词典 on leave

I'm not letting you leave until you tell me what's going on here.

你告诉我到底发生了什么之前我不会让你走的。

Culture tender leaves in culture medium of MS+2,4-D1.5mg/L+30g/L suger+0.7% agar pH5.8 for 20 days in darkness at 25℃, and then subculture to induced Ⅱ-type calli. Use forceps cutting the tissue to nubble with 2mm2, and put the tissue into Agrodacterium tumefaciens LBA4404 liquid supplemented with AS 100mg/L,then, co-culture 3 days, resume 7 days in MS+2,4-D1.5mg/L+30g/L suger+500mg/L cef, take to MS+2,4-D1.5mg/L+30g/L suger+100mg/L cef+10.0mg/L kanamycin culture 20 days in darkness. After that to make it polarize in MS+30g/L suger+100mg/L cef+10.0mg/L km. The percentage of km resistant callus was reached max after infection for 45 min, the average is 29.66%. Simultaneity, tender leave callus are infected 5 min by A. tumefaciens liquid in different negative pressure, and kept on 15 min in Agrodacterium tumefaciens liquid without negative pressure. Then screen out resistant callus and obtain transgenic plants. When the negative pressure is -0.05MP the percentage of km resistant callus was reached max, the average rate is 37.5%.

将心叶接种于MS+2.4-D1.5mg/L+30g/L 蔗糖，琼脂0.7%，pH5.8 培养基中25℃暗培养20d 后继代一次，诱导产生Ⅱ型胚性愈伤组织，用镊子将其夹碎成2mm2大小的小块，置入添加100mg/L AS 的根癌农杆菌LBA4404 菌液中，侵染时间为45min，共培养3 天后，转入MS+2.4-D1.5mg/L+30g/L 蔗糖+500mg/L Cef 培养基中恢复7天，再转入MS+2.4-D1.5mg/L+30g/L 蔗糖+100mg/L Cef+10.0mg/L Km 中，遮光培养20d后，置入其MS+30g/L 蔗糖+100mg/L Cef+10.0mg/L Km 分化，卡那霉素抗性愈伤组织获得率在侵染45min 时达到最大值平均为29.66%；同时以甘蔗心叶愈伤组织材料，通过循环水式真空泵，产生负压，设定不同的负压值，在农杆菌菌液中感染5min 后，在负压条件下继续侵染15min 后，筛选出抗性愈伤组织并获得转化植株，其中在负压为-0.05 时转化率达到最大值，其卡那霉素抗性愈伤组织获得率平均为37.5%。

Those who disobey will have to put on the ultimate badge of nudist dishonour — clothing — and leave.

违者将被给与裸体主义者的最终耻辱徽章--服装--离开。

WHAM! four nutcrackers followed by two sharp punches that leave him motionless on the ground.

其次是四个钳两个尖锐出拳，这使他一动不动地在地面上。

According to reports in Portugal, the Under-17 international has agreed to leave The Bescot Stadium for Old Trafford on a two-year deal after the two clubs came to a financial agreement.

来自葡萄牙的报道说，在两家俱乐部就转会费达成一致后，这位17岁以下的明星已经同意了曼联方面提出的两年合同。

Many people contract these companies when they are too ill to leave their houses on a daily or weekly basis for treatment.

不少人的合约，这些公司时，他们都过于轻率离开自己的房子，每天或每周治疗。

It'll leave you walking on cloud nine and everyone is going to know about it!

这些让你飘飘欲仙，所有人都明白这点。

If you don't want it, leave it on the edge of the plate.

假如你不喜欢的话，把它放在盘子一边。

Its not right to leave that on the ground.

Larry，你真是个有公德心的人。

I had to leave work on grounds of ill health.

我因病不得不辞去工作。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。