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The monoclonal antibody was produced by the method of inoculating BALB/c mice intraperitoneally with the hybridoma cells, and purified with protein A-Sepharose 4B affinity chromatography.3 Establishing of ELISA protocol to detect hBLySTwo kind of different ELISA protocols were tried to study the experiment condition of detecting hBLyS level. Streptavidin was used to indirect coating in protocal 1, but biotin-avidin system was used to enlarge the final reaction signal in protocal 2. The optimal reaction condition was chosen by the experiments. The sensitivity of protocal 1 was poor, but the linear relation of protocal 2 standard curve was excellent when the concentration of hBLyS was between 3.2~400 ng/mL. So a protocal was established which sensitivity can reach to nanogram.

建立检测hBLyS蛋白含量的ELISA方法选用2种ELISA方案对hBLyS蛋白含量的测定方法进行研究，方案一用链霉亲和素做间接包被，方案二用生物素-亲和素系统做终反应放大；优化选择2种检测方法的反应条件；方法一的敏感度较低：方法二在hBLyS蛋白浓度为3.2～400 ng/mL范围内，标准曲线的线性关系良好；从而建立了一种可检测hBLyS蛋白浓度为ng级水平的ELISA方法。

For tightening and receding of wefts, the conditions under which wefts recede from the back beam and move forward towards the front beam relative to warps are developed by combining equilibrium condition of forces of warp with critical slip condition of weft.

针对纬纱的打紧与回退问题，将经纱的力平衡条件与纬纱的临界打滑条件相结合，建立了纬纱相对于经纱向后梁一侧回退及向胸梁一侧前移的条件。

Evidence suggests that 1 under more stable stratification, the basic flow, if moving faster at low and high levels (particularly in the presence of jets there), allows a resulting meso-β unsteady wave to propagate eastward with respect to basic flow and even at greater velocity compared with it; 2 vertical windspeed shear of basic flow causes instabilities of the TWT perturbation; 3 considering the second derivative of basic-flow wind with respect to z (denoted by zz≠ 0 which is simply given as β* hereafter) the expression for the phase velocity of vortex Rossby wave is obtained, which is unidirectional in propagation with respect to basic flow; 4 VRoW has its physical origin from β*, i.e., from z-varying heterogeneities of y-direction averaged vorticity of the basic flow field; 5 VRoW phase velocity is associated with zonal wave number k, its energy is dispersive and the group velocity exists in the x direction; 6 when windspeed meets the condition of β*, TWT disturbance instability may be that of mixed VRoW and gravity wave; 7 if basic flow is subject to linear shear but does not meet the condition of β*, the TWT instability is that of inertia-gravity wave.

在大气层结比较稳定的情况下，如果基本气流在低层和高层较大(有可能存在低空急流和高空急流)，此时产生的β中尺度不稳定扰动相对于基流向东传播，甚至于快速向东传播。基本气流在垂直方向上的风速切变对于中尺度横波型的扰动起着不稳定的作用。如果考虑基流的二次切变，可以得到涡旋Rossby波的相速度表达式，涡旋Rossby波相对于基本气流是单向传播的。涡旋Rossby波产生的物理根源是基本流场的风速二次切变，亦即基本流场y方向的平均涡度在空间z方向上的不均匀所致。涡旋Rossby波的相速度与纬向波数也有关，它的能量是频散的，其在纬向x方向也存在群速度。在基本流场的风速存在二次切变时，横波型不稳定可能是混合的涡旋Rossby重力波的不稳定；而在基本流场的风速仅仅存在线性切变，不存在二次切变时，横波型扰动的不稳定则是重力惯性波的不稳定。

Pumping station forbay is an important part of pumping station ,and the hydraulic characteristic of the pumping station forbay has a direct influence on the efficiency of a pump. The efficiency of the pump unit can be uprated in the condition of well flow condition.

泵站前池是泵站的一个重要的组成部分，进水的水力特性对水泵性能有着直接的影响，良好的进水条件可以使水泵装置达到良好的运行状态，获得较高的效率。

A any stocks of the Products in saleable condition and in the possession of Distributor, shall be forthwith held to the order of Supplier and any such Products which have been paid for in full by Distributor shall be resold by Distributor to Supplier or such person as Supplier shall specify at , due allowance being made for old stock and any out of condition or unsaleable Products

a 可供销售并由经销商占有的任何库存产品，应由经销商替供应商占有，已由经销商全额付款的经销产品应当由经销商转售给供应商或者供应商所指定之人，对陈旧的存货、保存不当的经销产品或不能销售的经销产品在价格上应予以适当扣除；且

Under the microenvironments of fruit bagging,Alternaria had always been the major fungi in different bags,sites of fruit and weather conditions during the whole growing season. As the secondary major fungi,Penicillium had always been after July and emerged later in inner bag and cortical pore than other sites.In the bag with which the inner bag was red and waxing,Trichoderma was isolated first in pericarp without cortical pore and inner bag as well as later in cortical pore after August.In \'Kobayashi\' bag which inner bag was red and waxing, Aspergillus and Fusarium were isolated in pericarp without cortical pore and cortical pore after bag removed.Alternaria and Penicillium were isolated in unbagging fruit and \'Qianwei\' bag which inner bag was red and unwaxing and the date Penicillium was isolated in CK was 45d later than bagging treatment.Under high temperature weather condition,there were no differences in the fungi population structure on fruit surface among all treatments.And the fungi population structure on fruit surface in bagging apple was more than that of unbagging ones under overcast and rainy condition.

在苹果套袋微域环境下，不同育果袋、果实不同部位、不同时期、不同气象条件下的真菌主要是链格孢霉，且在套袋后的整个生长季均可分离到；青霉为第二大真菌，在7月份后均可分离到，在非皮孔部位的出现要早于内果袋和皮孔部位；8月份后在内袋红色涂蜡的育果袋上分离出了木霉，其先出现在非皮孔处和内果袋，然后出现在皮孔处；曲霉和镰刀菌仅在内袋红色涂蜡的&小林&袋的非皮孔部位和皮孔部位于摘袋后分离出；在内袋为黑色且未涂蜡的育果袋和未套袋果上仅仅分离出链格孢霉和青霉，且青霉分离出的时期要比套袋果晚45d。

Based on the crack control criterion, the deflection control criterion in ultimate state of service and the strength control in the ultimate state of load-carrying capacity and the nominal steel percentage of section of beam, the formulas for the calculation of the control moment capacity of T-beam and bridge under the limiting deflection condition、the limiting cracking condition and the ultimate strength state were given, respectively.

在提出名义配筋率概念的基础上，基于结构正常使用极限状态的变形控制、裂缝控制，承载能力极限状态下的强度控制，推算不同极限状态下被预测梁的控制弯矩，并建立了桥梁结构承载能力预测方程，从而完成桥梁结构实用动力预测全过程。

However, when the switches are working as linear mode, the relations between the wide output electromagnetic pulse and biased dc voltage, trigging condition, and a function between the transforming efficiency and biased dc voltage, trigging condition need father research.

然而，当开关工作于线性模式时，开关的输出电脉冲的宽度与偏置电压和触发条件的关系、开关的转换效率与触发条件及偏置电压确切关系还需进一步研究。

High neurotic testees cognitive flexibility is better under the condition of passive emotion than nagitive emotion condition.

高外倾被试在消极情绪状态下的认知灵活性显著低于在积极情绪状态下的认知灵活性。

Under the condition of passive emotion, testees cognitive flexibility is better than testees under the condition of nagitive emotion.

高神经质被试的认知灵活性显著低于低神经质被试的认知灵活性。

The dissecting of samples in group2 were difficult. The root of pulmonary artery and ascending aorta failed to be unfolded because fibrous tissue was tough, right and left fibrous trigone were too firm to be solved by hand. Cardiac muscle fibers couldn't be stripped along myofibrillar trajectory since they were prone to break because of their friability.

组2的心脏解剖困难，表现为纤维组织坚韧，游离肺动脉非常困难；徒手无法松解左、右纤维三角，肺动脉和主动脉根部的游离非常困难；心肌纤维坚硬、质脆，解剖时容易断离成碎块，无法沿纤维走行方向剥离。

We have battled against the odds in a province that has become increasingly violent.

我们对在一个争夺日益激烈省的可能性。