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ABSTRACT It is because that the excellent acceptors for maize transgenic engineering are insufficient in our country, especially in the southwest mountain areas of china and hereditary variation regularity for the two characters such as efficiency of embryonic callus induction and number of regenerating plant (these two characters were abbreviated to the nduction efficiency and number of regenerating in the following of the paper, respectively), which hint the maize culturing capacity, is not very clear. Therefore, aiming at picking out superior acceptors, we had made systematic researches on the two characters with combing traditional quantitative-character genetic analyzing methods such as single-factor genetic mating design, diallel crossing genetic design, genetic effect analyzing method and the modern molecular locating method such as QTLs'. The main results are followed.(1) 50 superior inbred lines and about 30 crosses in our country, especially in the southwest of China were used for identifying and selecting the superior genotypes in the above two investigated characters under the same culturing condition in 2000 and 2001. There was very significant difference among the genotypes in the both characters. But the two characters were not certainly related. Some genotypes such as 18-599 and 18-599 were very good in them. For some ones such as zong 31, induction was higher than 18-599 and 18-599 in the efficiency, but it was only 1/3 to the later in regenerating number. In some genotypes such as S37, R08, R15, P138, A318, induction efficiency was just about 3% and scarcely any regenerating plants were got. On the whole, hybrids acted better than inbreeds in the both characters.(2) Two kind of inbreeds were selected as parents of the Griffing's method 1. 18-599 and 18-599 and the inbred line zong 31 are one kind because they are not only superior in the characters of maize cross breeding, such as CA, resistance to disease and the important agricultural characters, but also excellent in transformation characters as the induction and regeneration.
针对我国、特别是西南山地所需玉米转基因工程育种优良受体极为匮乏和反应玉米幼胚培养能力的2个主要性状,即玉米幼胚胚性愈伤组织诱导率和胚性愈伤组织绿苗发生数的遗传变异规律十分不清楚的实际情况,本研究从筛选玉米转基因工程所需要的优良受体入手,采用单因素遗传交配设计、双列杂交遗传交配设计、世代基因效应等传统数量性状分析方法,以及现代分子标记定位主效QTL分析方法,对玉米幼胚胚性愈伤组织诱导率和胚性愈伤组织绿苗发生数等2个性状进行了较为系统的分析研究,取得以下主要研究结果:(1)于2000年和2001年通过对我国、特别是西南地区近50份优良自交系和近30个杂交组合,在相同培养条件下,对幼胚培养胚性愈伤组织诱导率和胚性愈伤组织绿苗发生数等2个幼胚培养能力性状进行了筛选与鉴定,发现玉米不同基因型具有完全不同的幼胚培养胚性愈伤组织诱导率和胚性愈伤组织绿苗发生数,但幼胚培养胚性愈伤组织诱导率与胚性愈伤组织绿苗发生数并不具有必然的相关关系,有的基因型,如自交系18-599和18-599在胚性愈伤组织诱导率和愈伤组织绿苗发生数等2个性状都表现相当优异;有的基因型,如自交系综31,仅幼胚培养胚性愈伤组织诱导率性状表现高于19-599和18-599,但在胚性愈伤组织绿苗发生数这一性状则与它们有相当大的差距,仅为19-599和18-599的1/3左右;有的基因型,如S37、R08、R15、P138、A318等玉米自交系不仅幼胚培养胚性愈伤组织诱导率很低,平均仅在3%左右,而且胚性愈伤组织绿苗发生数表现也很差,基本上没有分化成苗。
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According to the problems and shortages exited in the first national judicial cxalnination,author suggests that the system sheRid be perfected in the following three aspects:first,on the basis of lower condition for the entrance of the examination and lower matriculating score,We should keep the balance of right and obligation;second,we could deepen the judicial officials and personal system at the turning point of establishing the national judicial examination system;third,We should perfect the existing judicial unitary examination system through the combination of unitary examination and unitary study so that the purpose of practicing the national judicial examination system can be fulfilled much better.
针对首届国家统一司法考试存在的问题及不足,笔者建议从三处着手进行完善:首先,在放宽报名条件和降低录取分数线上应讲求&权义平衡&;其次,以国家司法统一考试制度为契机深化司法官人事制度改革;最后,以&统一考试+统一研修&的结合来完善现行的司法统一考试制度,使国家实行司法统一考试制度的目的能更好地实现。
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Wet-on-wet: The condition of printing on a multi-colour press that the succeeding coloured inks being overprinted before the previous ink has dried.
湿叠湿:在多色印刷机上,油墨尚还未干,就印上另一颜色油墨的情况。
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Wet-on-wet: The condition of printing on a multi-colour press that the succeeding coloured ink s being overprinted before the previous ink has dried.
湿叠湿:在多色印刷机上,油墨尚还未干,就印上另一颜色油墨的情况。
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Wet-on-wet: The condition of printing on a multi-colour press that the succeeding coloured inks being overprinted before the previous ink has dried.
湿叠湿:在多色印刷机上,油墨尚还未干,就印上另一颜色油墨的情况。每段预拼的箱梁安装在小平车上。
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Based on the path-state query technology, the target path-state is prejudged if the target path state fulfills the switching condition before the path-switching is executed; thereby, the success rate of path-switching is improved and the possibility that the path-switching has the influence on the storage performance of the system is reduced.
通过本发明实施例,可以在真正执行倒换前,预先判断是否满足倒换条件,从而提高路径倒换成功率,并降低路径倒换对系统存储性能产生影响的可能性。
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Therefore, baculovirus was the pathogen of this disease. When checking up on site, it was found that there were a lot of bateria in hepatopancreas and hearts. The healthy shrimps injected with homogenated tissue suspension of diseased ones died earlier and quicker than that injected with same suspension but eliminated bacteria. So, if shrimps injected with virus, then reinfected with bacteria, its condition will be worse and died quickly.
同时在活检时发现病虾肝胰腺及心脏压片中有较多细菌,将未除菌病虾组织浆悬液注射健康虾,比注射除菌病虾组织浆悬液的虾死亡早而迅速,所以受病毒感染后再继发细菌感染将加快病情恶化和死亡。
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Compared with Mg2+,the physiological cofactor,Mn2+has a slight activation effect on GTP-hydrolysis activity.The kobs of the exponential phase of the experiment curve of Mn2+is two-times higher than that of Mg2+under saturated condition.And the velocity of the steady state is lower than that of Mg2+.
相对于生理条件下的辅基Mg2+而言,Mn2+对Cdc42Hs酶活力有所激活,表现在饱和浓度时,实验曲线指数项的表观速率常数kobs有2倍左右的提高,而其稳态反应速度要低于Mg2+。
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Now the Condition of the above-written Guarantee is such that if the Contractor shall duly perform and observe all the terms provisions conditions and stipulations of the said Contract on the Contractor's part to be performed and observed in accordance with the true purport intent and meaning thereof or if on default by the Contractor the Guarantor shall satisfy and discharge the damages sustained by the Employer thereby up to the amount of the above-written Guarantee then this obligation shall be null and void but otherwise shall be and remain in full force and effect but no alteration in terms of the said Contractor or in the extent or nature of the Works to be executed, completed and defects in the Works remedied thereunder and no allowance of time by the Employer of the Engineer under the said Contract nor any forbearance or forgiveness in or respect of any matter or thing concerning the said Contract of the part of the Employer or the said Engineer shall in any way release the Guarantor from any liability under the above-written Guarantee.
现在在之上书面保证的条件是这样如果承包商将执行和适当地将观察前述合同的所有期限供应条件和规定在承包商的零件执行和被观察与真实的主旨意向符合和意思因此或如果在缺省由承包商保人将满足和将释放损失由雇主遭受因此由相当数量在之上书面保证决定然后这义务将是除消并作废的但否则将是和将依然是在充分的力量和作用但没有改变根据前述承包商或在工作被执行,被完成和瑕疵的程度或本质在工作由工程师的雇主补救在其下和没有任一件问题或事容限时间根据前述合同亦不任一忍耐或饶恕或尊敬关于雇主或前述工程师的部份的前述合同在任何情况下发行将保人从任一个责任在在之上书面保证之下。
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Owing to the lack of academic research and the perfection of legislation, it is very difficult to judge and handle some specific cases in the judiciary practice. Tracing back to its origin in history, drawing on the experience of other states and standing on the foothold of Chinese condition, the author believes that the official act, as a law term, has meaningful connotation.
但就目前研究现状来看,虽然多数学者根据《人民警察法》中一些对警察职务行为的规定,把职务行为与正当防卫和紧急避险并列为一种法定的排除犯罪性行为,但是,对职务行为的研究远远落后于对后二者的关注和热情,相关的论著和文章也是寥寥可数。
- 推荐网络例句
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This one mode pays close attention to network credence foundation of the businessman very much.
这一模式非常关注商人的网络信用基础。
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Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.
扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。
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There is no differences of cell proliferation vitality between labeled and unlabeled NSCs.
双标记神经干细胞的增殖、分化活力与未标记神经干细胞相比无改变。