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But when historical footstep strides 21 centuries really, people discovers surprisingly, weekly working hours is in person of quite a few infinite and outspread, exceeded 72 hours even, and many people were stripped however working right, be washed out flintily by the market and abandoned, and those weekly working hours are in ceaseless and outspread people is ego of promotion of bitter of work energetically of all the more however.

但历史的脚步真的迈入21世纪时，人们却惊奇地发现，相当多的人每周工作时间在无限延伸，甚至超过了72小时，而有不少人却被&剥夺&了工作的权利，被市场无情地淘汰和抛弃了，而那些每周工作时间在不断延伸的人们却是愈加发奋苦苦地&提升&自我。

Methods: hPDLCs of passage four were treated with 0.1% absolute ethyl alcohol, 10^(-10)mol/Lor 10^(-7)mol/L E, in DMEM supplemented with 10% FBS, 10mM β-glycerophosphate and 5μg/ml ascorbic acid for 24 hours, 48 hours and 72 hours.

采用剂量对照设计，评价四代hPDLCs分别在含有0.1%无水乙醇、10^(-10)mol/L E2或10^(-7)mol/L E2的矿化诱导培养基中培养至72h，时间点的观察荧光定量RT-PCR检测RANKL和OPC mRNA的表达水平。

Results In normal thermal group, rCBF was significantly decreased in 2 hours after SAH and the mortality rate is 35.88% in 24 hours. In hypothermal group, rCBF was also significantly decreased in 2 hours but to obviously lower extent compared with normal thermal group and with a mortality rate of 5.17%.

结果 常温组穿刺后2 h内rCBF显著降低，24 h 内死亡率35.88%；亚低温组术后2小时内rCBF显著降低，但降低幅度较SAH显著减少，24 h内死亡率为5.17%。

Methods The samples of hygienic protection beaded paint were chosen to conduct the soak experiment, incontinuous soak of 4 times for 96 hours(exchange the soaking aqua every 24 hours), continuous soak of 4 times for 96 hours were conducted respectively, and then compared these experimental results with that which was obtained with the standard soak method30 d,6 ti...

根据涉及生活饮用水卫生安全产品标准修订和制定协作会议精神，笔者对与饮用水接触的卫生防护涂料的浸泡实验的样品浸泡方法进行了实验研究。1材料与方法1.1原理根据与

The main injury of kidney was located in proximal tubular epithelial cell ,it peaked at 12 hours and 24 hours after reperfusion,the sporadic karyopyknosis and karyorrhexis could be seen,but the basement membrane preserved well.48 hours later,the restoration could be seen.7 days later it restored obviously.

肾脏损害主要以近曲肾小管上皮细胞为主，12h、24h损伤达到高峰，可见核固缩、核碎裂，但基底膜保留完好。48h开始出现修复现象。7d后明显修复。

Vascular endotheliocyte fluorescence staining and leukocyte fluorescence tracing method were applied, in combination with microscopic video system and computer imaging analyzing system, to observe in dynamic and quantitatively the influences of acupuncture on pia matral encephali microvascular morphology, density and blood flowing velocity at 3 hours, 6 hours and 24 hours after cerebral occlusion of meddle cerebral artery.morphology, density and blood flowing state at various time divisions observed directly microscopically and vascular endotheliocyte morphology and the state of brain tissue which near the microvascular after infusion with velocity in infarcted area at various time divisions measured in quantity in each group.microvascular endotheliocytes were colored worse and more fluorescent exudation appeared.

采用血管内皮细胞荧光染色及白细胞荧光示踪法，结合显微录像系统和计算机图像分析系统，动态定量地观察了针刺对大脑中动脉阻塞后3，6，24h软脑膜微血管形态、密度、血流速度的影响。主要观察指标：①从显微镜下直接观察各组大鼠不同时段软脑膜微血管的形态、密度、血流状况，以及注入荧光剂后血管内皮细胞形态和周围脑组织状况。②定量测量各组大鼠不同时段梗死区微血管密度、血流速度。结果：120只大鼠均进入结果分析。

METHODS: Rat bilateral olfactory bulbs and olfactory mucosa at 1/3 nasal septum were obtained, sliced, digested in trypsin, and made into monoplast suspension. At 1×109/L, cells were incubated in uncoated 25 cm2 culture flask. At 18-20 hours, cell suspension was moved into another uncoated 25 cm2 culture flask (the first differential adhesion). At 24 hours, cell suspension was moved into a poly-D-lysine-coated 25 cm2 culture flask or poly-D-lysine-coated 6-well culture plate (the second differential adhesion). At 48 hours, parenchyma cells were removed after a half of medium was changed.

完整取大鼠双侧嗅球及剪取鼻中隔后1/3嗅黏膜，剪碎后胰酶消化，制成单细胞悬液，按1×109 L-1密度接种于未包被的25 cm2玻璃培养瓶中，18~20 h后将细胞悬液转移至另一未包被的25 cm 2玻璃培养瓶中（第1次差速贴壁），24 h后再将细胞悬液转移至经多聚右旋赖氨酸包被的25 cm2塑料培养瓶或经多聚右旋赖氨酸包被的6孔培养板中（第2次差速贴壁），接种培养48 h后半量换液以去除杂细胞。

Cumulus-enclosed oocytes from follicles in prepubertal gilt ovaries from alocal abattoir were cultured in modified TCM199 with and without 10 IU/ml PMSGand hCG for 22 hours respectively.After fixation and dying in 1% acid orcein,itshowed that 77.6% oocytes reached MII stage.Maturated oocytes were denudedwith 1mg/ml hyaluronidase,and fertilized with fresh or thawed sperms in modifiedTBM for 6 hours,then cultured in NCSU23 for 14 hours.The fertilization rates were87.5% for fresh sperms,64.1% for thawed sperms.

抽取从屠宰场获取的猪卵巢中的卵母细胞，在加入和不加hCG和PMSG的mTCMl99培养液中分别培养22小时，地衣红染色发现77.6％的卵母细胞达到MII期；去卵丘细胞后在mTBM中用鲜精或冻精解冻后受精6小时，在NCSU23中培养14小时后固定染色发现受精率分别为87.5％和64.1％；电激活后在NCSU23中培养20小时后固定染色发现原核形成率为94.1％。

RESULTS: After 24 hours of preinduction, cells changed from spindle to polygonal or irregular, with many short rod-shape processes and 2-3 nucleoli. Whirlpool shape growth disappeared, and was disordered. After 1-3 hours of induction, cells gradually became round, with significant cell contraction, strong refraction, bipolar or pluripolar processes connected each other into a net. After 5 hours of induction, grades one and two branches were detected.

结果：预诱导24 h，胞体由原来的长梭形变成多角形或不规则形，伸出多个短棒状突起，可见2~3个核仁，细胞间漩涡状生长趋势消失，排列无规律；诱导1~3 h，细胞逐渐变圆，胞质收缩明显，折光性增强，双级或多极的突起互相连接呈网状；诱导5 h，突起出现一、二级分支。

The bcl-2/bax of Safflor group treated before ICH 24 hours ahead,after ICH 0 hour were lower than that of Safflor group treated after ICH 6,12 or 24 hours. When the treated group were injected Safflor after ICH 6,12,24 hours,The number of caspase-3 positive cell around

在不同时间给予红花注射液治疗脑出血大鼠，均能不同程度地促进脑出血大鼠神经功能的恢复，其中术后6h给药组治疗效果最好，其可能通过促进Bcl-2蛋白的表达，抑制Bax和Caspase-3蛋白的表达，从而抑制血肿周围的细胞凋亡。

In the days of sailing vessels the crew were afraid they would be becalmed on the ocean.

在使用帆船的时代，船员们担心他们会因为无风而无法在海洋上航行。

As long as foreign donors pay the PA's salary bill, few expect a new intifada.

只要外国继续为巴权力机构的薪水买单，希望发动新暴动的人便寥寥无几。