查询词典 od

G od gives every bird its food, but he doesn't throw it into its nest.

上帝给了鸟儿食物，但他没有将食物扔到它们的巢里。

The PCR products were examined by agarose gel electrophoresis. The target gene fragments were purified by gel extraction kit and ligated to cloning vector pMD18-T. The recombinant vectors were transformed into host strain E. coli K802 by lithium chloride method, screened and identified with PCR and restrictive enzymatic digestion. Their sequences were confirmed by DNA sequencing.(2) sTWEAK1 gene was subcloned into expression vector pProEx HTb and transformed into E. coli BL21. sTWEAK2 gene was subcloned into expression vector pMAL-C2x and transformed into E. coli TB1. The recombinant vectors were screened and identified with PCR and restrictive enzymatic digestion. The recombinant fusion proteins were induced to express with IPTG, detected by coomassie brilliant blue-stained SDS-polyacrylamide gel electrophoresis , and confirmed by Western blot analysis.(3) The sTWEAK1 fusion protein was purified with Ni-NTA Spin Kit.(4) The biological activity was assayed on transformed and tumor cells by microplate photometer after crystal violet or sulfur rodamine B staining.(5) The contents of IL-8 in the supernatant of 1990 cell cultures were determined by ELISA.(6) The morphological changes of the sensitive cells were observed by light and transmission electron microscopies.(7) The cell cycle and apoptotic rate were assayed by flow cytometry in 1990 and M85 cells.(8) The effect of fusion proteins on induction of NF-κB in 1990 and LOVO cells was detected with Dual-Luciferase Reporter Assay system.(9) The TWEAK gene was subcloned into Adeno-X Viral DNA with pShuttle vector and transfected into HEK293 cells by lipofectamine method.

(1)本研究用RT-PCR方法，从人组织细胞总RNA中扩增可溶性TWEAK胞外区（sTWEAK1和sTWEAK2）的cDNA序列及全长编码序列，用琼脂糖凝胶电泳分析PCR产物，胶回收目的基因片段，连接到pMD18-T克隆载体中，转化大肠杆菌K802，PCR和酶切筛选阳性克隆，全自动DNA测序验证序列；(2)sTWEAK1和sTWEAK2分别亚克隆到pProEx HTb和pMAL-C2x表达载体中，分别转化大肠杆菌BL21和TB1，PCR筛选和酶切鉴定，阳性克隆用IPTG诱导表达，表达产物用SDS-PAGE分析和Western blot验证融合蛋白；(3)用NTA-Ni Spin试剂盒初步分离纯化sTWEAK1融合蛋白；(4)用体外培养的肿瘤细胞和正常对表达产物进行活性检测，贴壁细胞用结晶紫染色法，悬浮细胞用磺酰罗丹明B染色法，酶标仪检测OD值；(5)敏感细胞用ELISA法检测细胞培养上清中IL-8的含量；(6)用光镜和电镜观察敏感细胞死亡和细胞凋亡情况；(7)用流式细胞仪分析表达产物对敏感细胞凋亡率和细胞周期的影响；(8)用双荧光素酶报告基因检测法，测定表达产物对敏感细胞NF-κB的影响；(9)用pShuttle穿梭质粒将TWEAK重组到腺病毒载体上，用脂质体转染法转染HEK293细胞，PCR鉴定重组质粒。

I'm walking down the line That divides me somewhere in my mind, On the border line od the edge And where i

我在路上走着，这条路在脑海中把我撕裂，在这界眼边缘，我独着

Plus 2 No's diagonal support braces made out of 76.1 mm OD (3.25mm thick –zinc coating as per BS 1387) MS pipe –total length of pipe 3 meters ; along with all fixing accessories like GI short winder, PVC caps , brace band , tension band , rail end cap,double clamp.

加上 2不支持括号对角线作出了七十六点一毫米外径（三点二五毫米厚锌层为每基站1387 ）质谱管道总长度为3米的管道；以及所有安装附件如消化道短络筒机，聚氯乙烯瓶盖，支撑带，张力带，轨端帽，双钳。

Topic The influence of art erial m echanics on ce ntral blo od press ure, cardi ovascular risk a nd

题目 动脉机制对中心血压，心血管危险性及冠状动脉灌注的影响

The device is made up of two sections: a cascaded antialiasing + switched capacitors 8th bandpass filter for precise RDS band selection and a demodulating section that performs the extraction od RDS data stream and clock, to be further pro- cessed by a suitable RDS decoder.

该装置是由两部分组成：一层叠抗锯齿+开关电容器用于精确RDS的波段选择第八带通滤波器和解调部分，它执行外径RDS数据流和时钟提取，将进一步支持处理过的由一个解码器适合铁路发展策略。

Compared with Simvastatin Tablets and the capsule of Xuezhikang,the effects of Wendan decoction on serum triglycerides,serum total cholesterol,serum LDL-C and serum HDL-C in adult rats with chronic hyperlipidemwere observed after30dates.Result:By using pharmacological meth-od,the results revealed that Wendan decoction could effectively reduce serum of TC,TG,LDL-C levels in the rats with chronic hyperlidemia,it has raisen the concents of blood HDL-C and its function was more effective than the capsule of Xu-ezhikang.

除空白对照组喂基础饲料外，其余各组均采用高脂乳剂灌饲方式建立大鼠高脂血症模型，喂养10日后眼球取血，测得受试动物血清中总胆固醇、总甘油三酯、低密度脂蛋白胆固醇显著升高，说明高脂血症模型复制成功。30日后，运用药理学方法，观察温胆汤对高脂血症成年大鼠血清中TC、TG、LDL-C、高密度脂蛋白胆固醇含量的影响，并与西药辛伐他汀、中成药血脂康进行比较。

Yes! it refers to your woman or wife.because in the garden of Eten, Eve was created from the rib od Adam.

应该是指女人，在创世纪中，上帝趁亚当睡着的时候从他身上取下一根肋骨，造了夏娃。

After flocculation the cells of Klebsiella pneumoniae in the floes grew well(OD 13.5)and were used repeatedly.

絮凝细胞再发酵实验表明：絮凝沉淀中的菌体仍然可以重复利用。

But it certainly shouldn't be idealised either, and nor should anyone argue that traditional Muslim theocracy--which protects the other "peoples of the book", the Christians and Jews, but also treats them as second-class citizens--is any basis for a modern state. The od theocracy served a

但也不应该将其理想化，同时人们也不应该争辩穆斯林传统的神权政治（它也保护《可兰经》上记载的其他民族，如天主教徒和犹太教徒，但他们只是二等公民）是这个现代国家的基础。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。