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The results showed that the transformation efficiency of the leaves precultured for 9 d on preculture medium, infested in OD value equal to 0.7 bacterium medium for 10 min, and then co-culture in dark conditions and co-cultured medium with 100μmolL^(-1) acetosyringone for 3 d respectively; was optimal.

结果表明,预培养9d的悬铃木叶片在OD600值为0.7的根癌农杆菌菌液中浸染10min后,再在含有100μmolL^(-1)的乙酰丁香酮的共培养培养基上黑暗条件下共培养3d叶片的遗传转化效率最高。

MTT assay FAK signaling pathway inhibitor genistein on human corneal epithelial cell cytotoxicity;RT-PCR detection of human corneal epithelial cells adhesion to fungus at different times,extracellular matrix protein including laminin,fibronectin,FN glass,Ⅳcollagen,transmembrane protein integrinαⅤ,integrinβ1(ITGβ1),as well as the FAK signaling pathway FAK1,FAK2 and Paxillin gene expression;Western blot detection of the signal transduction pathway adhesion-associated protein ITGβ1,FAK and PAX expression and the inhibition of genistein. Immunocytochemical method was used to observe the LN,FN and FAK expression in human corneal epithelial cells during interaction with the fungues;Laser scanning confocal microscope had a cell positioning on FN,FAK and PAX,observed the changing of the human corneal epithelial cytoskeleton after stimulated by fungues;Quantitatived by flow cytometry to detect of human corneal epithelial cells with PAX at ITGβ1 fungal expression after adhesion;Optical microscopy quantitied the fungues and human corneal epithelial cell adhesion and recorded to determination the integral optical density afrer adhesion;Scanning and transmitted electron microscope observed the changing of cell ultrastructure after fungues and human corneal epithelial cell adhesion.

第一部分真菌激活人角膜上皮细胞FAK信号转导通路的体外实验研究将三种常见致病真菌(白色念珠菌、烟曲霉菌和茄病镰刀菌)分别与人角膜上皮细胞共孵育,MTT法检测FAK信号通路抑制剂染料木黄酮的对人角膜上皮细胞的细胞毒性作用;RT-PCR检测真菌黏附人角膜上皮细胞后不同时间细胞外基质层连蛋白、纤连蛋白、玻连蛋白、Ⅳ型胶原、跨膜蛋白整合素αV、整合素β1(ITGβ1),以及FAK信号通路中FAK1、FAK2和桩蛋白基因的表达情况;Western blot的方法检测黏附信号转导途径相关蛋白ITGβ1、FAK和PAX的表达,以及染料木黄酮对真菌刺激人角膜上皮细胞FAK信息通路活化的抑制作用;免疫细胞化学方法观察人角膜上皮细胞与真菌相互作用过程中LN、FN和FAK的表达;激光共聚焦显微镜对FN、FAK和PAX进行了细胞定位,并观察真菌刺激后人角膜上皮细胞骨架的变化;流式细胞仪定量检测人角膜上皮细胞ITGβ1与PAX在真菌黏附后表达的改变;光学显微镜观察真菌与人角膜上皮细胞黏附数量,记录并测定了黏附后积分光密度值OD扫描及投射电镜观察了真菌与人角膜上皮细胞黏附后,细胞超微结构的改变。

The leaders of Foreign Languages School responde positively to the call of the Department od Education to carry out the teachers' virtue-building activities in the whole school.

外国语学校积极响应市局号召,在全校范围内开展了轰轰烈烈的师德师风建设活动。

To study the effect of specific electromagnetic wave on the growth of microorganism,this paper uses TDP and TDP without radiation board to irradiate growing vintage saccharomycete and E.

为了研究特定电磁波对微生物生长的影响,用TDP和取下辐射板的TDP照射正在生长中的酿酒酵母菌菌液和大肠杆菌菌液,并检测菌液的OD值。

Migratory songs wing from my heart and seck their nests in your voice od love.

飘泊的歌儿从我心中飞去在你爱的呼声里寻找窠巢。

Methods 100 mg of muscle of rat corpse was preprocessed before trimethylamine was extracted with anhydro-methylbenzene, then trinitrophenol was added into extracts, and yellow compounds emerged. The optical density was detected by spectrophotometer at 410 nm and the concentration of TMA-N was calculated using the standard curve. Results There was good linear correlation (R^2=0.9991, P.001) between OD and the concentration of TMA-N in the range of 1~10 mg/L.

大鼠肌肉组织样本100 mg经预处理后,将三甲胺抽提于无水甲苯中,与苦味酸作用,形成黄色的苦味酸三甲胺盐,用分光光度计在410 nm处检测吸光度,标准曲线法计算TMA-N含量。

The rats in control group were given distilled water, and the rats in experimental groups were given different doses of capsule(4.0、2.0、1.0g/kg), then enhance the capillary permeability of abdominal skin of rats by using xylic acid,and the OD numerical value was detected.

补肾利浊胶囊高、中、低剂量组分别给予相应剂量的补肾利浊胶囊(分别为2.0、1.0、0.5 g/kg),模型对照组及正常对照组予等量蒸馏水。

The rats in control group were given distilled water, and the rats in experimental groups were given different doses of capsule(4.0、2.0、1.0g/kg), then enhance the capillary permeability of abdominal skin of rats by using xylic acid,and the OD numerical value was detected.

(1)抗炎实验:将40只小鼠随机分为补肾利浊胶囊高、中、低剂量组及对照组各10只,补肾利浊胶囊高、中、低剂量组分别给予相应剂量的补肾利浊胶囊(分别为4.0、2.0、1.0 g/kg),对照组给予蒸馏水,采用二甲苯酸致小鼠腹部皮肤毛细血管通透性增高,测定光密度值;(2)镇痛实验:40只小鼠分组及给药同抗炎实验,采用腹腔注射醋酸致小鼠出现扭体反应,纪录扭体次数并计算镇痛百分率;(3)抗前列腺炎实验:将雄性大鼠50只随机分为补肾利浊胶囊高、中、低剂量组及模型对照组及正常对照组,各10只。

Methods ①anti-inflammatory trial: 40 rats were randomly divided into control group, high-dose bushenlizhuo capsule group, middle-dose bushenlizhuo capsule group and low-dose bushenlizhuo capsule group(10 rats in each group).the rats in control group were given distilled water, and the rats in experimental groups were given different doses of capsule(4.0、2.0、1.0g/kg), then enhance the capillary permeability of abdominal skin of rats by using xylic acid,and the od numerical value was detected.

(1)抗炎实验:将40只小鼠随机分为补肾利浊胶囊高、中、低剂量组及对照组各10只,补肾利浊胶囊高、中、低剂量组分别给予相应剂量的补肾利浊胶囊(分别为4.0、2.0、1.0 g/kg),对照组给予蒸馏水,采用二甲苯酸致小鼠腹部皮肤毛细血管通透性增高,测定光密度值;(2)镇痛实验:40只小鼠分组及给药同抗炎实验,采用腹腔注射醋酸致小鼠出现扭体反应,纪录扭体次数并计算镇痛百分率;(3)抗前列腺炎实验:将雄性大鼠50只随机分为补肾利浊胶囊高、中、低剂量组及模型对照组及正常对照组,各10只。

Normally the two timers are in step, and the external cues te nd to regularise the internal clocks to the more convenient 24 hour peri od.

正常情况下两个定时系统是同步的,外部的信号会将体内时钟调节到更方便的24小时周期。

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According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm~150rpm时取得最大值,此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世,形体几无变化。

When I was in school, the rabbi explained everythingin the Bible two different ways.

当我上学的时候,老师解释《圣经》用两种不同的方法。