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To understand the infectivity by porcine endogenous retrovirus with porcine skin fibroblast cell in vitro and in vivo, porcine skin fibroblast cell established by our laboratory were co-cultured with neo/HEK293 cell for the infection of RERV in vitro, and were subcutaneously transplantated to SCID (severe combined immuno-deficiency) mice for the infection of PERV in vivo, laying the foundation for valuation of biologic safety of xenotrans-plantation. The event of neo/HEK293 cells infected by PERV occurred during co-culture of porcine skin fibroblast cells with neo/HEK293 cells, expanding the rang of the infection of porcine endogenous retrovirus. Afterpig cells transplantated subcutaneously in SCID mice, the microchimerism (78.57%) of pig cells occurred widel, and there was phenomena of integration of PERV provirus (85.71%) in several organs or tissues remote from the injected sites, indicating infection of PERV in SCID mice in vivo. yet, there is no evidence of active viral replication in analysis of PERV env RNA of these tissues or organs.
为了解猪皮肤成纤维细胞PERV在体外和体内的感染性,通过建立猪皮肤成纤维细胞系,将所建细胞系与人胚胎肾293细胞体外共培养,并移植于严重联合免疫缺陷鼠皮下进行猪皮肤成纤维细胞PERV的体外和体内感染性实验,结果表明,猪皮肤成纤维细胞与人胚胎肾细胞共培养过程中,猪内源性逆转录病霉感染人胚胎肾细胞,进一步证实和拓宽了猪细胞PERV感染人细胞的范畴;猪皮肤成纤维细胞移植SCID鼠皮下后,导致SCID鼠发生猪细胞微嵌合(78.57%)和PERV在体内感染(85.71%)并且波及远离移植部位的多种组织或器官,但是并未检测出SCID鼠组织中表达PERV env RNA。
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Cd and Zn mainly occurred in weak acid soluble fraction, reducible fraction and oxidisable fraction, while Pb and Cu mainly occurred in residual fraction.
结果表明,Cd和Zn主要以可提取态存在(即弱酸溶解态、可还原态和可氧化态),而Pb和Cu均以残渣态为主要存在形式。
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Water area and cropland were the main land cover types that reed converted. Lower places in north and east parts of Baiyang Lake were the ones occurred the conversion between reed land and water area, and higher places in northwest and southwest occurred the conversion between reed land and cropland.
研究结果表明,16年间,就整个白洋淀而言,苇地变化经历了&落-起-落&的过程,水域与耕地是苇地变化的两个主要方向,苇地与水域的转变主要发生在地势较低的北部与东部地区,苇地与耕地的转变主要发生在西北与西南的地势较高地区。
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Myzus persicae also occurred periodically as that of the turnip aphid, but appeared several months later and almost constantly occurred in November during the three years.
7黄条叶蚤发生盛期因年而异,但以5-12月之虫数较1-4为高。(8)伪菜蚜每年9月至次年2月为其发生盛期,高举期受8月降雨量多寡之影响,而出现在9、10或次年1月。
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Under conventional N fertilization and irrigation condition, N2O emissions during 2001-2002 and 2002-2003 in winter wheat and summer maize rotation system were 1959 g N hm-2 and 1250 g N hm'2, respectively; and 68%-86% of N2O emission occurred in summer maize growth season, the emissions mainly occurred in 2 weeks after N fertilization and irrigation. The highest flux rates were 5.06 mg m-2 d-1 and 5.60 mg m-2 d-1 in the rotation period of 2001-2002 and 2002-2003, which were happened at the ten-extended leaf stage in summer maize season when top-dressing N fertilizer was applied.
在冬小麦/夏玉米轮作体系农民传统的水肥管理处理中,2001-2002和2002-2003两个轮作周期内测得的N_2O的排放量分别为1959 gN hm~(-2)和1250 gN hm~(-2),N_2O的排放主要集中在每次施肥和灌水之后的两周内,其中夏玉米十展叶期追肥后两周内N_2O的排放速率最高,2002年和2003年分别达到5.06 mg m~(-2)d~(-1)和5.60 mg m~(-2)d~(-1)。
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Hypha and arbuscular colonization have same orders in the two breeds, fastigium occurred in summer and low occurred in late spring and early summer, spore peak produced in summer in the Rhizospere of Puerraria lobata. Spore density had positive correlation with abuscular colonization, the minimum value appears in the winter, and the maximum appears in the summer; consist with the growth prosperous of lobata.
菌丝与丛枝定殖率在两品种间变化规律基本一致,高峰期都出现在冬季,低峰期则出现在春末夏初,夏季为葛藤根区AM 真菌的产孢高峰期,孢子密度与丛枝定殖率呈正相关,最低值出现在冬季,最高值出现在夏季,与葛藤的生长旺季相一致。
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All sinus arrest, atrial arrhythmia and atroventricular block occurred in the course of syncope or the portent of syncope. Ventricular premature beats occurred before syncope or the portent of syncope.
其中复合的窦性停搏、房性心律失常、房室传导阻滞均发生在晕厥或晕厥先兆症状出现时;而室性早搏出现于晕厥或晕厥先兆前;交界区性心律失常出现在晕厥或晕厥先兆当时或以后,平卧位后均逐渐消失。
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Arrhythmias included second degree atrioventricular block in 3 patients, paroxysmal ventricular tachycardia in 3 patients and frequent ventricular premature beat in 3 patients. Cardiac syncope occurred in 2 patients with Ⅱ°AVB and in a patient with VT.Significant coronary stenosis related to ischemia occurred in 5 patients, and coronary artery was normal in 1 patient.
结果:9例患者心绞痛发作时除有典型的心电图ST段抬高的变化外,均伴有严重的心律失常,其中二度房室传导阻滞3例、室性心动过速3例、频发室性早搏3例。2例Ⅱ度AVB及1例VT伴发作性晕厥。1例Ⅱ度AVB伴黑矇。6例行冠脉造影提示缺血相关血管狭窄5例;1例冠脉造影结果正常。
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The results showed that truncations of T-DNA borders, insertions of filler DNA and the formation of inverted and direct repeat occurred during T-DNA integration. From the cleavage site of the T-DNA borders, no truncations occurred for nine left borders, most of the left borders deleted less than 35bp and most of the right borders truncated to 21bp.
分析了233个T-DNA左边界和190个T-DNA右边界与水稻基因组交接位点的序列,结果表明T-DNA在整合过程中不仅伴随着左右边界序列的缺失及&filler DNA&的插入,而且还以单拷贝或多拷贝的形式插入在基因组中的同一个位点。
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Results Various side effects resulting from antituberculous agents were confirmed in 302 cases (32.7%),liver injury,acoustic nerve injury, blood system injury, gastrointestinal response of them were primary (82.1%).Stopped treatment rate was 14.6%.77% in side effects occurred within 2 months after administration, but the blood system injury occurred after 3 months.
结果:抗结核药物副作用302例(32.7%),以肝损害、位听神经损害、血液学异常及胃肠反应为主(82.1%),因副作用致终止治疗率14.6%。77%的副作用出现于给药的2个月内,血液学异常的50%以上在用药的3个月后出现。
- 推荐网络例句
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According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.
曝气设备的动力效率在叶轮转速为120rpm~150rpm时取得最大值,此时氧利用率和充氧能力也具有较高值。
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The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.
稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世,形体几无变化。
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When I was in school, the rabbi explained everythingin the Bible two different ways.
当我上学的时候,老师解释《圣经》用两种不同的方法。