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GV oocytes with normal shape were not obtained in the group which was stored at 20℃ for 24 h and at 25℃ for 14 h; A blastula rates (64%) of all groups in average was gotten in culturing 2-cell in vitro, and there is no significant difference in temperature and time of storage. After transplantating some of the 2-cell embryos which were obtained from GV oocytes stored at 15'C for 8 h, normal infant mice could be obtained.

在20℃下保存24h和25℃下保存14h时，难以获得形态正常的GV期卵；体外受精获得的2细胞期胚经体外培养，总体上有64%的胚胎发育至扩张囊胚，未见有保存温度和保存时间的显著影响，且利用在15℃保存8h后的GV卵获得2细胞期胚的移植获得正常新生小鼠。

The research results indicated that the best base was KOH, the best acid was H3PO4 and their neutralizing products were the necessary nutrients for foliar fertilizer; 12.4 L of foliar fertilizer were obtained in per kg of shrimp offal, chitin was obtained in 13.8% yield, crude protein was obtained in 23.2% yield using this method; This multifuctional foliar fertilizer was an abundant nutritional multifunctional foliar fertilizer which contained 15 kinds of amino acids, shimp cephalin, astacein and many trace elements. The concentration of calcium was 38.9 mg/L, the concentration of magnesium was 55.4 mg/L, the concentration of potassium was 20.0 g/L, the concentration of nitrogen was 4.5 g/L and the concentration of phosphorus was

研究结果表明：(1)处理虾下脚料的最佳碱是KOH，最佳酸是H3PO4，其中和产物是叶面肥必需的营养素；(2)本工艺不仅每Kg虾下脚料可制备虾叶面肥12.4L，而且可回收13.8％甲壳素和23.2％粗蛋白质；(3)经分析测定虾叶面肥中，含钙(Ca2+)38.9 mg/L，含镁(Mg2+)55.4 mg/L，含钾20.0 g/L，含氮4.5 g/L，含磷 78.9 g/L，还含有15种氨基酸及虾脑磷脂、虾红素及多种微量元素等，是一种营养丰富的多功能叶面肥。

Were done and no positive reaction was found.5. The DNA of the young leaves of 132 Bt transgenic plants was extracted to do PCR Five positive inversion plants were obtained and the inversion rate was 3.8%.6. The DNA of fire positive inversion plants obtained in Dl generation was extracted and tested. Two stable positive inversion plants were obtained in D2 generations.7 The way of flourescent mirror flat was used to observe the pollen tube extension circumstance in the flower post after self-pollination.

检测了132株，没有发现阳性反应。5、对转入Bt基因的132株植株的幼叶提DNA，作PCR检测。D1代获得5株阳性转化植株，分别是90035的4株，85—593的1株，转化率为3.8％。6、将D1代获得5株阳性转化植株的种子在温箱中发芽，提取DNA检测，得到D2代稳定遗传的阳性转化植株2株。7、应用荧光镜制片法观察大豆自花授粉后花粉管在花柱中延伸情况。

Romberg first use of the method is integral for integration, Then the results obtained by using the interpolation method were obtained Lagrange polynomial interpolation polynomial interpolation and Newton, re-use of least squares fitting of thinking obtained polynomial, the last of these different types of polynomial, identify their respective strengths and weaknesses.

首先运用Romberg积分方法对给出定积分进行积分，然后对得到的结果用插值方法，分别求出Lagrange插值多项式和Newton插值多项式，再运用最小二乘法的思想求出拟合多项式，最后对这些不同类型多项式进行比较，找出它们各自的优劣。

Weather fresh onion or onionskin dry powder, the yield of polysaccharides obtained by microwave-assisted freeze thawing was the most, and the content of polysaccharicle obtained by utrasonic wave was the most. The yield of polysacchalides obtained by microwave-assisted freeze thaucing in onionskin was 3.58%(the content of polysaccharides was 63.9%); and its yield in fleshy parts was 3.15%(the content of polysaccharides was 62.0%).

不管是新鲜洋葱肉质部分还是洋葱皮干粉，微波辅助冻融法提取的多糖的得率最高，超声波法提取的多糖的含量最高，其中微波辅助冻融法提取洋葱皮中多糖得率为3.58%(含量为63.9%)；肉质部分多糖得率为3.15%（含量为62.0%）。

Combining with the National Natural Science Foundation ofChina(No.50478032), following researches were done in this thesis: 1 The variation regularity of the ultimate anti-bending capacity of therectangular-sectioned beam with different corrosion has been obtained through thequalitative analysis of 48 beams (32 corroded beams and 12 comparing beams) withaccelerated corrosion; 2 The conclusion has been obtained that the strain of corroded steel bar andconcrete no longer fit in with the assumption of plane section through the analysis ofthe strain data, the strain relation of steel bar and concrete in the mid-span section ofvariously corroded beams at ultimate has been obtained. Through the building of newgeometric relation, the expression of the height of compressive area in the corrodedbeam with rectangular section has been deduced, and the formula with corrosion rateas the main variable for the anti-bending capacity of the corroded concrete beam hasbeen suggested and verified by experiment.; 3 The influence of steel bar corrosion and the concrete regression to the flexureductility coefficient of the corroded structure component has been analyzed. Based onthe relation of steel bar and concrete at yield and ultimate, the expression for theflexure at yield and ultimate of corroded reinforced concrete beam, and the model forthe calculation of the flexure ductility has been built.

本文结合国家自然科学基金项目(50478032)"既有钢筋混凝土桥梁时变可靠度研究"，主要进行了以下研究工作： 1通过对48根快速锈蚀钢筋混凝土矩形截面梁(32根锈蚀梁和12根对比试验梁)数据的定性分析，得到了不同锈蚀率下，锈蚀钢筋混凝土矩形截面梁极限抗弯承载力的变化规律； 2通过对应变数据的分析，论证了锈蚀构件钢筋和混凝土的应变不再符合平截面假定的结论，并通过回归分析得出了不同锈蚀率的螺纹钢筋梁和光圆钢筋梁在极限弯矩作用下，跨中截面处钢筋应变和钢筋处混凝土应变的关系，通过构造新的几何关系，得出了锈蚀钢筋混凝土矩形截面梁计算受压区高度的表达式，既而得出了以锈蚀率为主要变量的锈蚀钢筋混凝土矩形截面梁的正截面抗弯计算建议公式，并通过试验数据加以验证； 3分析了钢筋锈蚀和混凝土劣化对锈蚀构件曲率延性系数的影响，结合钢筋屈服和极限弯矩两种状态下钢筋和混凝土的应变关系，分别得到了锈蚀钢筋混凝土矩形截面梁屈服曲率和极限曲率的计算表达式；既而推导出曲率延性系数的计算模型。

Then through optimum thrust force acceleration governing equation obtained by Pontryagin maximum principle and the reasonable simplification, the optimum low thrust under the orbit elements is obtained. Finally, the optimum transfer orbit analytic solution is obtained by integrating the equations.

应用Pontryagin极大值原理建立航天器最优推力加速度的控制方程，对方程进行合理的简化，只考虑它们的一次项，忽略其它的高次项，从状态方程中得出小推力对轨道根数产生的摄动影响，然后分别对它们进行积分，从而得到最优转移轨道的解析解。

It is shown that two-component Wadati-Konno-Ichikawa equation, i.e. a generalization of the wellknown WKI equation is obtained from the motion of space curves in Euclidean geometry, and it is exactly a system for the graph of the curves when the curve motion is governed by the two-component modified Korteweg-de Vries flow. At the same time, a n-component generalization to the WKI equation is obtained. Also, starting from the motion of curves, mKdV and its symmetry recursion operator is exhibited explicitly; two- and n-component mKdV systems are obtained. It is shown that WKI systems are gauge equivalent to mKdV systems. The two-component WKI equation admits an infinity number of conservation laws and a recursion formula for the conserved densities is given by considering an eigenvalue problem together with introducing an appropriate transformation.

在二维和三维欧氏空间上，我们从空间曲线运动出发，推导出了mKdV方程以及它的用以生成高阶对称的递归算子；推导出了多元mKdV方程以及二元和多元WKI方程，并证明了WKI系统和mKdV系统的规范等价性；尔后，通过考虑特征值问题，并引入一个恰当变换，给出了二元WKI方程的用以计算无穷多守恒密度的递归公式，从而证明了二元WKI方程的守恒可积性；系统地分析了两种mKdV方程的Painleve性质，并分别给出了两种不同形式的二元和n元mKdV方程的共振点出现的规律。

The system utilized four laser scanners along lengthwise direction of the log. Data are obtained in every ten degrees when log is turning, in each cross section, 36 data are obtained. Then the data obtained were processed and an outline of the cross section of the log was developed on computer screen. Afterwards, the largest inscribed circles for log cross sections were determined according to the principle that three points can determine a circle. Secondly, the points located on the cross sections were linked up along some log generative in order to simulate the three-dimension log outline. At last the largest right circular cylinder were optimized and the optimum turning center was determined by means of projective theory.

具体作法是：在木段长度方向均匀设置四个激光传感器，当木段旋转时，起动激光传感器，进行数据采集，每隔10~0扫描一个点，在各横断面圆周上共采集36个点的数据，由计算机处理采集的数据，并绘制出原木横断面的几何图形，再根据三点定圆的原理，确定出最大内接圆；其次，模拟原木三维廓形，即将原木相邻横断面上离散点沿着母线方向连线，由计算机分析处理，绘制原木三维廓形；最后，采用投影法，优化出原木最大内接圆柱体，并确定原木回转中心线。

Chapter four studies the chaotic responses in a system consisting of simple pendulum and harmonic oscillator under bounded noise excitation. Firstly, the Melnikov function of the two-degree-of-freedom system under Hamiltonian perturbation is derived. The essential condition of the autonomous system for the probable onset of chaos is obtained, the Poincare maps of the system under small Hamiltonian perturbation and the effect of increasing perturbation on the Poincare maps are studies. Then for the non-autonomous system under damping and harmonic or bounded noise excitation, the largest Lyapunov exponent and Poincare maps are calculated. From the analysis of the largest Lyapunov exponent, the critical criterion for the onset of chaos, and the conclusion that the threshold of bounded noise amplitude for the onset of chaos in the system increases as the intensity parameter of Wiener process increases are obtained. The result from the analysis of Poincare maps is in agreement with that obtained from the Largest Lyapunov exponent. The effect of varying damping coefficient and intensity parameter of Wiener process is also analyzed.

第四章研究了有界噪声激励下的两自由度单摆—谐振子系统的混沌运动，首先推导了该两自由度系统仅在Hamilton扰动下的Melnikov函数，得到该自治系统可能产生混沌的必要条件；研究了该系统在小的Hamilton扰动和增大摄动情形下的Poincare截面；然后对有阻尼、谐和或有界噪声激励下的非自治系统数值计算了其最大Lyapunov指数和Poincare截面；从Lyapunov指数分析得到了这个两自由度系统产生混沌运动的临界条件及产生混沌的临界激励幅值随Wiener过程强度参数值的增大而增大的结论，Poincare截面分析的结果亦符合Lyapunov指数分析的结论；研究了Wiener过程强度参数、阻尼系数变化对Poincare截面的影响。

The circulation, consumption and reproduction of the trilogy have not only testified to its historical significance and literariness, but also invested it with new meanings.

该三部曲的流通、消费与再生产不仅验证了其历史意义及文学性，而切还赋予它以新的意义。

If he thought that he could buy me off, he would be wrong.

如果他认为他可以收买我，那他就大错特错了。