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Methods The rats were given with Huanggui enema by enemata and the paw swelling and pain threshold of rats were observed; the rabbits were given with Huanggui enema by enemata and the fever reaction of rabbits was observed.

用黄桂灌肠剂给大鼠灌肠，观察其对角叉菜胶所致大鼠足跖肿胀、大鼠痛阈值的影响。用黄桂灌肠剂给家兔灌肠，观察其对家兔发热的影响。

The results revealed that no excision was observed in p3E1.2-CBW-2,but two excisions were observed in p3E1.2-CBW-2,though the frequency was very low(0.00025%).

但HaPLE1构建的质粒具有0.00025%的剪切频率，不过观察到的剪切位点均不是piggyBac转座子的经典剪切位点TTAA。

Materials and Methods Thirty SD rats, feeding in clearing grade standard, were classified into five groups at random. Application of transmission electron microscopy, scanning electron microscopy, light microscopy and immunohistological staining meth- ods, normal morphology of outflow pathways was observed and component of extra cel- lular matrix was defined. The effects of 50 IU of TNF-α given intracamerally on the morphology of outflow pathways and other tissues of anterior segments, expression of trabecular stromelysin and the change of components of trabecular extra cellular matrix were observed in 24h, 3days, 7days and 14days after injection.

中文题名TNFα对鼠眼房水外流影响的形态学观察副题名外文题名 The effect of TNFα on morphology of outflow pathways in rat eyes 论文作者王大博导师王竫华教授学科专业眼科学研究领域\研究方向学位级别博士学位授予单位青岛大学学位授予日期2002 论文页码总数90页关键词青光眼房水外流通道肿瘤坏死因子-α馆藏号BSLW /2003 /R775 /1 目的在观察SD大鼠眼房水外流通道形态及确定其房水外流通道细胞外基质成份的基础上，探讨房水中肿瘤坏死因子α浓度的增加是否能诱导小梁细胞基质溶解素的表达和分泌，进而启动小梁细胞外基质的重塑过程。

After bleeding sloped, the hemoglobin amount in films or gauzes were measured to determine the hemostatic effect Using a randomized design, one incision in each animal was treated with C-CM membrane, the influence of C-CM film on the growth of bacteria (Staphylococcus aureus ATCC 27853. Escherichia coli ATCC 25922. Monil lia albicans ATCC 14053)were observed in vitro with solid medium. Rusults: It was observed that C-CM membrane had no toxic effects on surrounding tissue and was slowly degraded after implanted into rat leg muscular porch ; The results of hemostatic experiment showed that C-CM film adhered well to incisions.

结果：C-CM膜植入动物肌肉中，周围组织无明显炎症，未发现组织变性和坏死，可在组织内缓慢降解，具有良好的组织相容性，6周后反应接近外科缝合丝线组；止血实验结果发现：C-CM膜组和纱布组的出血时间分别为83.5±9.333s和117±15.9275s，血红蛋白光度吸收值为0.9204±0.7559和1.9764±0.7205，二组比较差异显著(P＜0.05)，说明C-CM膜有一定的止血作用；促进创面愈合实验结果显示：不同时间段C-CM膜组肉芽组织较对照组厚，其中胶原纤维含量亦较多，C-CM膜组的创面愈合时间早于空白对照组，伤口愈合较快；抑菌实验显示C-CM膜对三种菌产生接触性抑制。

Two different signals, whichwere brown and blue, reacted with the substrate (DAB and NBT/BCIP) separately, could be observed in the WSSV infected haemocyte. The result showed that in the lightly infected tissues infected haemocytes predominated in all infected cells. However in the heavily infected tissues the number of normal haemocyte decreased obviously and infected cell with strong positive intensity increased, and cell debris and tissue necrosis were observed.

应用这种双重免疫组化方法观察了被WSSV感染的血细胞在几种组织中的分布情况，发现在轻度感染组织中被侵染的血细胞在所有被感染细胞中占绝大多数，而在高度感染组中血细胞数目明显减少，细胞变形，出现许多的细胞碎片，同时被病毒感染的细胞数目增多，染色强度增强，组织坏死严重。

Methods: The CD34+ cells and AC133+ cells were gathered by MiniMACS, then the cell factors were divided into 6 groups. Through the ex vivo comparison cultivation under different cell factor combination stimulation, expansion of the CD34+ and AC133+ selection cells was observed and the clone formation of the CD34+ and AC133+ cells was also observed by using Half-solid Methyl cellulose under different cell factor stimulation (in7d, 14d, 21d.). The rate of cell apoptosis was determined.

常规富集人骨髓CD34+及AC133+细胞，应用本研究组设计并已经证实的细胞因子组合方式，对人骨髓CD34+及AC133+富集细胞进行体外对照培养，分别观察CD34+和AC133+富集细胞的扩增情况；应用甲基纤维素半固体培养法，观察不同组别培养7、14、21d CD34+和AC133+富集细胞的集落形成情况及细胞凋亡率。

Methods CD34(superscript +) cells and AC133(superscript +) cells were gathered by MiniMACS, then the cell factors were divided into 6 groups. Through the ex vivo comparison cultivation under different cell factor combination stimulation, expansion of the CD34(superscript +) and AC133(superscript +) selection cells was observed and the clone formation of the CD34(superscript +) and AC133(superscript +) cells was also observed by using Half-solid Methyl cellulose under different cell factor stimulation (during 7d, 14d, 21d.). The rate of cell apoptosis was determined.

常规富集人骨髓CD34及AC133细胞，应用本研究组设计并已经证实的细胞因子组合方式，对人骨髓CD34及AC133富集细胞进行体外对照培养，分别观察CD34和AC133富集细胞的扩增情况；应用甲基纤维素半固体培养法，观察不同组别培养7、14、21d CD34和AC133富集细胞的集落形成情况及细胞凋亡率。

In meiosis of pollen mother cells of the tetraploids and the hexaploids, only bivalents were observed in most of the populations, although in several populations or individuals a few univalents or tetravalents appeared. In the hexaploids, hexavalents were not observed.

四倍体和六倍体的花粉母细胞减数分裂染色体构型在大多数居群都为二价体(四倍体中30个二价体，六倍体中45个二价体)，少数居群或是个体除二价体为主外还出现单价体和四价体，六倍体类型没有出现六价体构型。

The cells full of crystals observed in orient tobacco were verified to be crystal idioblasts. The crystals observed by SEM were multiangle.

研究表明，香料烟株内结晶细胞是一类特化的含晶细胞，细胞内充满晶体，晶体形状似角锥状，质体等被挤到细胞边缘。

I have observed from cities for 30 years," he writes. Using a refractor "with a 5-inch f/5 Jaegers objective, I have observed 350 of 600 deep-sky objects that I know are possible, most of them many times.

我已经在城市里观测天体30年了，&他写道，&使用一台5英寸的折射镜，我已观测了我所知的500个深空天体中的350个，其中大多数都不只一次。

On the other hand, the more important thing is because the urban housing is a kind of heterogeneity products.

另一方面，更重要的是由于城市住房是一种异质性产品。

Climate histogram is the fall that collects place measure calm value, cent serves as cross axle for a few equal interval, the area that the frequency that the value appears according to place is accumulated and becomes will be determined inside each interval, discharge the graph that rise with post, also be called histogram.

气候直方图是将所收集的降水量测定值，分为几个相等的区间作为横轴，并将各区间内所测定值依所出现的次数累积而成的面积，用柱子排起来的图形，也叫做柱状图。