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Methods: Adopting pig bile culture medium, the inhibiting or killing effects on pig bladder worm in vitro were observed;by using the methods of xylene-induced ear inflammation in mice and granuloma swelling induced by cotton ball in rats, the effects of anti-inflammation were observed;by using the methods of body twisting and hot-plate in mice, the effects of analgesia were observed. Through the mice auricle microcycle, the rats thrombus law in vitro and bleeding time, clotting time determination to the mice, the function of invigorating the blood were observed;adopting the penicillin abdominal cavity injection method, the mice epilepsy model were prepared, and epilepsy mice brain organization MDA content and SOD, ATPase vigor be measured by using chemical colorimetry.

利用猪胆汁培养基观察该方在体外对猪囊尾蚴的抑制或杀灭作用;采用二甲苯所致小鼠耳肿胀法、大鼠棉球肉芽肿法以观察该方的抗炎作用;采用热板法和化学刺激扭体法观察该方的镇痛效果;通过小鼠耳廓微循环法、大鼠体外血栓法和对小鼠出、凝血时的测定观察该方的活血作用;采用青霉素腹腔注射法制备小鼠癫痫模型,并利用化学比色法检测癫痫小鼠脑组织中MDA的含量及SOD、ATPase的活力。

Growth of cells were observed daily by MTT assay, the status of cell proliferation were observed by Plate colony formation, and the cell cycle of the cells were observed with flow cytometry .

应用MTT比色法绘制细胞生长曲线;克隆形成实验了解细胞增殖情况;流式细胞仪观察细胞周期。

In this research, the SOLO (Structure of the Observed Learning Outcome) Taxonomy presented by Australian scholars Biggs and Collis (1982) was adopted, and students studying the fifth and sixth grades in Taipei area were chosen as research targets.This study was primarily implemented with a quantitative analysis and supported by a qualitative analysis. In order to carry out the research, a questionnaire had been designed with criteria suggested by Jones et al.(2000), including "describing data","organizing and reducing data","representing data" and "analyzing and interpreting data." This questionnaire consisted of open-ended questions, and after analyzed with qualitative methods, the responses were classified and scored based on standards set in SOLO Taxonomy. Then the quantitative method "Partial Credit Analysis" from Rasch model was adopted in examining the reliability, validity and consistency of outcomes from the qualitative analysis.

提出的统计思维架构-「描述资料」、「组织及简化资料」、「呈现资料」、「分析资料」作为评量试题的向度,题型为开放性问答题,在区分学童的答题表现方面,采用质的分析,参照SOLO(Structure of the Observed Learning Outcome)分类法的分类标准,将学童不同的答题反应进行分类,给予不同的分数,最后再采用Rasch model中的部份给分模式进行量化分析,以量化数据佐证质性分析的信度与效度,以及不同给分的吻合度。

According to the LC-P line of each of insecticide in the mixture and the formula of co-toxicity factor, several expected mortality and the region of additive action (expected mortality±20% expected mortality) were calculated, and according to observed mortality of mixture, the 95% confidence interval was calculated, LC-P line of expected mortality with the region of additive action and LC-P line of observed mortality with the 95% confidence interval were drawn, it was found that there was overlap between the region of additive action and 95% confidence interval and two lines crossed each other when co-toxicity coefficient was more than 100 and the co-toxicity factor was less than 20, which meant there was no significant differences between expected mortality and observed mortality, and that there was on or a few overlap between the region of additive action and 95% confidence interval when co-toxicity coefficient was more than 100 and the co-toxicity factor was more than 20 or co-toxicity coefficient was less than 100 and the co-toxicity factor was less than 20, which meant there was significant differences between expected mortality and observed mortality.

根据单剂的LC-P线和共毒因子公式,求出混剂的期望死亡率和"相加作用区间"(期望死亡率±20%期望死亡率),根据混剂的实测死亡率求出95%置信区间,画出期望LC-P线及"相加作用区间"和实测LC-P线及"95%置信区间",发现当共毒系数大于100、共毒因子小于20时,实测LC-P线和期望LC-P线彼此交缠,期望LC-P线的"相加作用区间"和实测LC-P线的"95%置信区间"能高度重叠,表明两条LC-P线之间没有毒力差异;当共毒系数大于100、共毒因子大于20,或者共毒系数小于100、共毒因子小于-20时,实测LC-P线的"95%置信区间"和期望LC-P线的"相加作用区间"只有少量重叠或完全不重叠,体现出了实测LC-P线和期望LC-P线对供试害虫的毒力差异。

The notable proliferation was not observed by eyes in the local of injection. The infiltration of inflammation cells and mild proliferation of fibrocyte around dura mater was observed by HE stained in 4 and 8 weeks after injection. Infiltration and exudation of inflammation cells was observed by HE stained in epidural nerve root. Compared with group A, no changes of group B, C and D were observed under specific stained. Proliferation of type Ⅱ collagen fibers around dura mater was seen under immunohistochemical stained in 4 and 8 weeks after injection. There is no significant demyelination changes under LFB stained. The thickness and shape of the myelin sheath in epidural nerve root was not regular under transmission electronic microscopy in 4 and 8 weeks after injection. Fibroblast was also seen there. In nerve endometrium, macrophage could be seen under TEM, myelinated nerve fiber changed significantly, but nonmyelinated nerve fiber changed mildly. When 8 weeks, the changes of group D is smaller than the group B and C.

给药局部肉眼观察未见明显的纤维组织增生;HE染色可见B、C、D三组给药后四周及八周时硬膜内外均有炎细胞浸润,纤维细胞轻度增生,硬膜外神经根内有炎细胞浸润及炎性渗出;特殊染色B、C、D三组同A组相比未见有脊髓及神经根的改变;免疫组化染色,给药后四周及八周时,硬膜内外均有Ⅱ型胶原纤维增生;固兰染色B、C、D三组未见有明显脱髓鞘改变,与A组相比无明显异常改变;电镜观察B、C、D三组在给药后的四周及八周时,表现为硬膜外神经根内髓鞘厚薄不一,形状不规则,可见成纤维细胞,神经内膜中可见有巨噬细胞;粗大的有髓神经纤维变化明显,无髓神经纤维受累较轻;八周时电镜下D组改变较B、C两组为轻。

This dissertation involves the study of the growth dynamics of colloidal particles under unequilibrium and irreversibility condition. Firstly, it is of interest to investigate whether the ideal diffusion or reaction-limited aggregation universal behavior can be observed for the initially polydisperse practical system of basic yttrium carbonate as well. The fractal structure of basic yttrium carbonate aggregate cluster was observed using transmission eletron microscopy. The aggregation kinetics of basic yttrium carbonate colloids was studed using dynamic 1ight-scattering techniques. At 1.00mol/l NaCl solution the fast diffusion-limited aggregation behavior was observed with an increase in the aggregate size over time described by a power law. The aggregates are characterized by a fractal dimension D=1.82. The disordered and ramified cluster is consisted of primary colloidal particles and tenuous and more open. These are in good agreement with those observed for other colloidal system such as colloidal gold (1.86), silica (1.85) and polystyrene latex (1.82) in diffusion-limited aggregation from the static and dynamic light scattering by Lin. Therefore ideal diffusion-limited aggregation behavior can be observed in practical polydisperse system as well. Smoluchwski and fractal theories were used to obtain the aggregation rate constant for initially polydisperse system for the first time.

本论文研究胶粒的非平衡不可逆聚集动态学,分为两个部分,第一部分是通过实验研究一个新的初始多分散的碱式碳酸钇胶粒实际体系中,聚集标度性质的普适性,即使用透射电子显微技术观测聚集粒子簇的分形结构,使用动态激光光散射研究碱式碳酸钇胶粒的聚集动力学,胶粒带电而产生静电排斥作用使得碱式碳酸钇胶粒处于稳定状态,当加入电解质NaCl溶液时,碱式碳酸钇胶粒间的Debye-Hückel屏蔽距离缩短,从而降低静电排斥能垒,引起胶粒发生不可逆聚集,加入电解质NaCl的浓度为1.00mol/l时,碱式碳酸钇胶粒进行快速聚集,经历扩散控制聚集机理,形成结构疏松、更为开放的分形聚集粒子簇,其分形维数为1.82,碱式碳酸钇胶粒聚集增长动力学遵循幂函数增长规律,与Lin等使用静态激光光散射和动态激光光散射测量硅胶(1.85)、金胶(1.86)和聚苯乙烯胶乳(1.82)在扩散控制聚集状态形成聚集粒子簇的分形维数值以及粒子簇聚集增长的动力学规律相一致,表明胶粒聚集标度性质的普适性质在初始多分散的实际体系中也是存在的,首次由动态激光光散射的实验数据得到初始多分散胶粒分形粒子簇的聚集速率常数,并且其值与Smoluchowski聚集速率理论预期相符。

Methods: Mice were inoculated with tumor cells. Inhibitory effect of transfection with pCH510 on murine tumor origined from different inoculative dose and inhibiting effect of immediate transfection pCH510 after chemotherapy on tumor were observed, respectively. By cell culture technique, the influence of chemotherapeutic drug to activation of marcrophages and lymphocytes after i.p. injection of drug was observed. By cell counting method, the kinetics of the change of number of peripheral blood immunocytes induced by administration of chemotherapeutic drug was observed. The inhibitory effect of transfection with pCH510 five days after chemotherapy on murine tumor was observed.

采用瘤细胞接种建立小鼠肿瘤模型:通过基因转染,观察pCH510对不同接种量所形成的小鼠肿瘤的抑制作用以及小鼠肿瘤化疗后立即进行pCH510转染的抑瘤效果;采用细胞培养技术,观察小鼠体内注射化疗药物后,其对小鼠腹腔巨噬细胞和脾淋巴细胞激活功能的影响;采用细胞计数的方法,观察化疗药物所致小鼠腹腔巨噬细胞和外周血免疫细胞数量变化的动力学;另外小鼠肿瘤化疗后第5天进行pCH510转染,观察抑瘤效果。

Comparing with the result of methyl iodide in intense laser field, some differences are observed:(1) at the same laser field intensity, the highest charged fragment ion of methyl bromide was Br(superscript 3+), lower than I(superscript 6+) of methyl iodide;(2) the dehydrogenation channel was observed in the multiphoton dissociation or Coulomb explosion of methyl bromide, but was not observed in the case of methyl iodide;(3) HBr(superscript +) was observed, but there was no similar channel in the case of methyl iodide;(4) for methyl bromide, the valid charge distance of Coulomb explosion increases with the product of p and q; while in the case of methyl iodide, the distance remained almost the same;(5) the producing channel of CH(superscript + subscript m)(m=0, 1, 2) is different from the ionization-dissociation of methyl iodide in which the stepwise dissociation of CH3(superscript +) was thought to be the main channel, CH(superscript + subscript m)(m=0, 1, 2) of methyl bromide are mainly from the products of the direct dissociation of the dehydrogenated parent ions instead of the stepwise dissociation of CH3(superscript +).

与碘甲烷在强场中的实验结果对比发现:(1)在相同的激光场强下,碘甲烷电离解离的最高价碎片离子为I(上标 6+)而溴甲烷为Br(上标 3+);(2)溴甲烷质谱中存在母体离子的脱氢产物CHBr和CHBr(上标 2+),而对于碘甲烷,没有检测到这些通道,C-I键首先断开;(3)质谱中存在H^79Br和H^81Br,而碘甲烷的电离解离中不存在HI产物;(4)溴甲烷库仑两体爆炸的有效电荷间距随着两碎片电荷乘积的增大而增大,而对于碘甲烷此间距几乎不随电荷乘积变化;(5)CH(m=0, 1, 2)的主要生成通道可能与碘甲烷不同,不是来自CH3的顺序脱氢,而是来自脱氢母体离子的直接解离。

Results: In cellular soluble protein electrophoresis,specific band about 75 KDa was observed in 593 strain.The differences of protein expression at 85,60,50,40KDa between 593 strain and 18 strain were observed.In extracellular soluble protein electrophoresis,specific bands at 75 KDa and 40 KDa were observed in 593 strain;specific band at 50 KDa was observed in 18 strain.The difference of protein expression amount in about 60KDa between 593 strain and 18 strain was observed.

结果:菌体可溶性蛋白电泳图中见593号菌株在75 KDa左右存在特异表达的蛋白条带,且二者在85、60、50、40 KDa左右蛋白表达量上存在差异;菌外可溶性蛋白电泳图中见593号菌株在75、40 KDa左右有特异表达的蛋白条带,而18号菌株在50 KDa左右有特异表达的蛋白条带,二者在60 KDa左右蛋白表达量上存在差异。

The research results show that the spectral variation of visible radiation from the target observed by the synchronous satellite is the same as that of the direct sunlight radiation, the spectral radiant flux of the target observed by the low orbit satellite is three orders higher than that observed by the synchronous satellite in magnitude, and the spectral radiant flux of the target observed by the midorbit satellite is one order higher than that observed by the synchronous satellite in magnitude.

研究结果表明:在可见光波段,目标对同步卫星辐射的光谱变化规律与太阳直射辐射的光谱变化一致,低轨卫星上观测目标的光谱辐射热流比同步卫星上观测的结果大3个数量级,中轨卫星上观测目标的光谱辐射热流比同步卫星上观测的结果大1个数量级。

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The absorption and distribution of chromium were studied in ryeusing nutrient culture technique and pot experiment.

采用不同浓度K2CrO4(0,0.4,0.8和1.2 mmol/L)的Hoagland营养液处理黑麦幼苗,测定铬在黑麦体内的亚细胞分布、铬化学形态及不同部位的积累。

By analyzing theory foundation of mathematical morphology in the digital image processing, researching morphology arithmetic of the binary Image, discussing two basic forms for the least structure element: dilation and erosion.

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Have a good policy environment, real estate, secondary and tertiary markets can develop more rapidly and improved.

有一个良好的政策环境,房地产,二级和三级市场的发展更加迅速改善。