查询词典 nutrient cells

Results of the experimental groups: Comparison with the control groups, in 6h and 8h groups eminences of ependymal cells with cobblestone-like appearance were seen, their microvilli were further richer, and a number of microholes could be found on their base. In body part of SFO cell membrane of some ependymal cells were broken completely, their cytoplasm was no longer seen, and cell nucleus located at one side of the cell, 5-7μm in diameter and dividing lobe-like in shape. Beside those cells some ependymal cells appeared many folds on the top of surface. In 2h and 16h groups the observation results seemed no significant differences from the corresponding control groups.

实验组结果：与对照组相比，在6小时和8小时实验组中，可见成团的鹅卵石状室管细胞隆起于表面，微绒毛更为丰富，微绒毛根部可见到若干微细小孔，在SFO体部的一些室管膜细胞的胞膜完全破裂，胞质几乎荡然无存，仅剩胞核居于细胞的一侧，直径有5.7μm，呈分裂叶状；与这些细胞邻近的室管膜细胞表面最高处多呈现皱襞。2小时与16小时的实验组的观察结果与对照组相比未发现明显差异。

Methods Cells were maintained in DMEM medium with 10% fetal bovine serum. Five days before the beginning of experiments, the cells were seeded in phenol red-free DMEM medium containing 5% charcoal dextran-treated FBS. The cells were harvested, and seeded in 6-well culture plates or in 75ml flacks. After NP, BisA and DBP treatments for 72h, the cells were harvested, and mRNA and protein expression of PCNA, bcl-2 and bax were detected by reverse transcription-polymerase chain reaction and immunohistochemistry, respectively.

方法］T47D细胞在含10%胎牛血清的DMEM培养液中进行常规传代培养，实验前将细胞转移至无酚红DMEM（含5%活性碳葡聚糖苷处理过的FBS）中继续培养5d，收集细胞，PBS洗涤后接种於内置盖玻片的6孔板或75ml培养瓶中，用32×10^(-7)mol/L NP、32×10^(-7)mol/L BisA及32×10^(-6)mol/L DBP对细胞分别处理72h，用半定量RT-PCR技术观察这3种化合物对核增殖抗原PCNA、bcl-2及bax mRNA表达的影响，并用免疫组化方法对结果进行验证。

ES cells were maintained on gelatin-coated dishes in Iscove's modified Dulbecco medium containing 0.1 mmol/L of nonessential amino acids, 2 mmol/L of L-glutamine, 0.1 mmol/L of monothioglycerol, 15% fetal bovine serum, and 1000 U/mL leukemia inhibitory factor. Embryonic bodies were firstly formed by ES cells with hang drip method in IMDM without LIF. Six-day-old EBs were trypsinized and the cells were resuspended at a concentration of （3-4）×109 L-1 cells per 1 mL of a 1:1 mixture of culture medium and Matrigel?

实验方法：将鼠胚胎干细胞株接种于被覆明胶的培养瓶内，加入含0.1 mmol/L非必需氨基酸、2 mmol/L L-谷酰胺、0.1 mmol/L二羟基丙硫醇、15%胎牛血清、1 000 U/mL白血病抑制因子的IMDM培养液。3 d后采用&悬滴法&使细胞在不含白血病抑制因子的上述IMDM培养液中形成胚胎体，6 d后胰酶消化得到胚胎干细胞，浓度调整为（3~4）×109 L-1，与Matrigel？

Methods: The CD34+ cells and AC133+ cells were gathered by MiniMACS, then the cell factors were divided into 6 groups. Through the ex vivo comparison cultivation under different cell factor combination stimulation, expansion of the CD34+ and AC133+ selection cells was observed and the clone formation of the CD34+ and AC133+ cells was also observed by using Half-solid Methyl cellulose under different cell factor stimulation (in7d, 14d, 21d.). The rate of cell apoptosis was determined.

常规富集人骨髓CD34+及AC133+细胞，应用本研究组设计并已经证实的细胞因子组合方式，对人骨髓CD34+及AC133+富集细胞进行体外对照培养，分别观察CD34+和AC133+富集细胞的扩增情况；应用甲基纤维素半固体培养法，观察不同组别培养7、14、21d CD34+和AC133+富集细胞的集落形成情况及细胞凋亡率。

PC84045 human lung carcinoma cells (PC84045 HLCCs) were mixed with the BM cells activated by anti-CD3 McAb and IL-2 and then CD3-ALTO4 bi-specific McAbs was put into the cell suspension. The mixed cells were cultured for 3 days in presence of IL-2 and IL-3. Tumor cell colony formation method in half-solid culture system was used to assess the purging efficiency of the activated T cells to PC84045 HLCCs.

先用抗CD3单抗和IL-2激活肺癌患者骨髓中的T淋巴细胞，然后按不同比例将人肺癌细胞株PC84045细胞掺入到骨髓细胞中，再加入能同时识别CD3抗原和人肺癌细胞表面抗原的双特异抗体CD3-ALTO4，在IL-2和IL-3存在下共同培养3d后测试。

Methods CD34(superscript +) cells and AC133(superscript +) cells were gathered by MiniMACS, then the cell factors were divided into 6 groups. Through the ex vivo comparison cultivation under different cell factor combination stimulation, expansion of the CD34(superscript +) and AC133(superscript +) selection cells was observed and the clone formation of the CD34(superscript +) and AC133(superscript +) cells was also observed by using Half-solid Methyl cellulose under different cell factor stimulation (during 7d, 14d, 21d.). The rate of cell apoptosis was determined.

常规富集人骨髓CD34及AC133细胞，应用本研究组设计并已经证实的细胞因子组合方式，对人骨髓CD34及AC133富集细胞进行体外对照培养，分别观察CD34和AC133富集细胞的扩增情况；应用甲基纤维素半固体培养法，观察不同组别培养7、14、21d CD34和AC133富集细胞的集落形成情况及细胞凋亡率。

There is not a significant difference in these groups by one-way analysis of variance. 2 Among 51 cases with lung cancer, 7 specimens showed malignant cells in bronchial lavages, including 4 cases in central lung cancer and 3 cases in peripheral lung cancer. Concordant results were observed in 20 cases. 6 specimens showed malignant cells on cytological analysis, and NMSP was positive in at least one gene tested. 14 samples did not show malignant cells on cytological analysis, and the NMSP results were correspondingly negative. The results of cytology and NMSP were discordant in 31 samples. 30 samples were cytologically negative, but NMSP positive in one or more genes. In addition, there was one case that the cytology was positive for malignant cells, but the NMSP was negative in all of the three gene tested.

51例肺癌患者支气管灌洗液细胞学检查发现肺癌细胞7例，其中27例中心型肺癌患者4例支气管灌洗液发现肺癌细胞，24例周围型肺癌患者3例支气管灌洗液发现肺癌细胞。20例支气管灌洗液细胞学检查和过甲基化检测得到一致结论。6例支气管灌洗液发现肿瘤细胞，过甲基化分析至少一个基因阳性。14例灌洗液未发现肿瘤细胞，三个基因过甲基化分析均为阴性。31例支气管灌洗液细胞学检查和过甲基化检测得到不一致结论，30例细胞学检查为阴性，但过甲基化分析一个或一个以上基因为阳性。1例细胞学检查阳性但过甲基化分析阴性。

Interleuk in 2 (IL-2) is an important cytokine released from activated T lymphocytes, which activated lymphokine actived killer cells and natural killer cells , and promoted cytokine production of helper T Cells and natural killer cells , and stimulated T cell growth in vitro and so on . It plays a important role in body immune regulator.

白细胞介素2主要是由激活的T淋巴细胞产生的一类重要的细胞因子，具有激活淋巴因子活化的杀伤细胞和自然杀伤细胞，刺激T辅助细胞和自然杀伤细胞产生细胞因子和促进细胞因子的繁殖以及刺激T细胞在体外生长等功能，因此它是在免疫调节中有重要作用。

At the same time, the process of the wound healing was observed histologically, and the regeneration cycle of epidermal cells and the temporal change in inflammatory cells were measured. Results Inflammatory cells infiltrated into wound surface were neutrophils, followed by macrophages and lastly lymphocytes. Epidermal cells proliferated most actively on PBD 3 and the mitoses of them increased significantly on day 7 after burn. The gene expression of PDGF, PDGFR and EGFR reached peaks on PBD 1 and the gene expression of EGF and TGFβ-R2 were highest on PBD 3. In addition, the gene expression of TGFβ-R1 and TGFβ1 increased significantly on PBDs 5 and 7 respectively.

结果 炎性细胞到达创面依次为中性粒细胞、巨噬细胞和淋巴细胞；表皮细胞周期变化为伤后3天细胞增殖活跃，伤后7天细胞分裂明显增多；伤后1，3天PDGF、PDGFR(血小板源性生长因子受体)和EGFR基因表达明显增强，伤后3，5天EGF、TGFβ-R2基因表达增强，伤后5，7天TGFβ-R1(转化生长因子β受体1)基因表达增强，伤后7，10天TGFβ1基因表达增强。

Objective:The aim of the experiment is to search for the regulation that the hepatic stem cells abnormally differentiate into cancer cells. In the experiment,the variating regulation of PCNA,c-kit and c-myc in the formation and development of HCC will be interpreted with immunohistochemistry and RT-PCR. So,the initial mechanism that hepatic stem cells abnormally differentiate into cancer cells from the levels of cell,protein and gene will be clarified. The last,the inhibition to HCC with ulinastain will be studied,from which,a new way of clinical prevention and treatment for HCC will expect to be explored.

二、目的本实验旨在探讨以卵圆细胞为代表的肝干细胞在HCC发生、发展过程中在肝组织内分布规律，并通过免疫组化、RT-PCR等技术阐明PCNA、c-kit、c-myc在HCC发生、发展过程中的动态演变规律，从细胞、蛋白、基因三个水平初步研究卵圆细胞向癌细胞异常分化的机制，同时观察乌司他丁对HCC发生、发展的抑制作用，为肝癌的临床预防和治疗探索新的途径。

Great. What repertoire did you see?

太棒了。你看的是什么剧目？

I prepared not to accept such ridiculousness:"Please," I said to him.

我可不想理会这套荒谬的东西："请你通情达理一点儿。"